TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1244
AB  - INTRODUCTION: Following orthopedic surgery, bacterial infection may lead to significant complications related to
inflammation in the peri-implant region, including the potential for implant loosening. To address this concern, the
surface of metallic implants was modified through the application of bioactive and antibacterial coatings, aiming to
mitigate these issues.
EXPERIMENTAL: HAP/PVA/CS/Gent coatings was single-step electrophoretically deposited from four-component
aqueous suspension containing 1 wt.% hydroxyapatite powder (HAP, particles < 200 nm particle size, Sigma-Aldrich),
0.1 wt.% poly(vinyl alcohol) (PVA, medium molecular weight 89 to 98 kDa, 99 % hydrolysed, Sigma-Aldrich), 0.05 wt%
chitosan powder (CS, medium molecular weight, 190 to 310kDa with 75 to 85 % deacetylation degree, Sigma-Aldrich), and
aqueous gentamicin sulfate solution (Gent, concentration 50 mg/ml, Sigma-Aldrich) on titanium plates (Sigma-Aldrich).
Antibacterial activity of HAP/PVA/CS/Gent coating was evaluated against Staphylococcus aureus TL (culture collection-
FTM, University of Belgrade, Serbia) and Escherichia coli ATCC 25922, while kinetics of antibacterial activity was monitored
according to our previously published data [1]. In vitro cytotoxicity assay was performed towards two fibroblast cell lines
of different origin-mouse origin cell line (L929 (ATCC CRL-6364)) and human lung origin cell line (MRC-5 (ATCC CCL-171)) [2].
To evaluate the statistical significance of the biological assay results (antibacterial activity, cytotoxicity and ALP activity),
one-way analysis of variance (ANOVA) followed by a multiple comparisons posthoc test was used.
RESULTS AND DISCUSSION: HAP/PVA/CS/Gent coating exhibited strong antibacterial effect against both S. aureus
and E. coli, especially pronounced against S. aureus, causing bactericidal effect. Cytotoxic effect of HAP/PVA/CS/Gent
coating was not pronounced in investigated MRC-5 and L929 cell lines. MRC-5 fibroblast cells in contact with
HAP/PVA/CS/Gent doubled alkaline phosphatase levels compared to their contact with the control samples
(HAP/PVA/CS), indicating good osteogenic properties.
CONCLUSIONS: Electrophoretically deposited HAP/PVA/CS/Gent bioceramic coatings on titanium, demonstrated
significant potential as implants in orthopedic practice, functioning as drug carriers. Not only do they possess
antibacterial properties, but they also exhibit no adverse effects on living tissue.
PB  - Beograd : Savez hemijskih inženjera Srbije
C3  - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
T1  - Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application
EP  - 59
IS  - 1s
SP  - 59
VL  - 78
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Đošić, Marija and Janković, Ana and Stevanović, Milena and Grujić, Svetlana and Matić-Bujagić, Ivana and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2024",
abstract = "INTRODUCTION: Following orthopedic surgery, bacterial infection may lead to significant complications related to
inflammation in the peri-implant region, including the potential for implant loosening. To address this concern, the
surface of metallic implants was modified through the application of bioactive and antibacterial coatings, aiming to
mitigate these issues.
EXPERIMENTAL: HAP/PVA/CS/Gent coatings was single-step electrophoretically deposited from four-component
aqueous suspension containing 1 wt.% hydroxyapatite powder (HAP, particles < 200 nm particle size, Sigma-Aldrich),
0.1 wt.% poly(vinyl alcohol) (PVA, medium molecular weight 89 to 98 kDa, 99 % hydrolysed, Sigma-Aldrich), 0.05 wt%
chitosan powder (CS, medium molecular weight, 190 to 310kDa with 75 to 85 % deacetylation degree, Sigma-Aldrich), and
aqueous gentamicin sulfate solution (Gent, concentration 50 mg/ml, Sigma-Aldrich) on titanium plates (Sigma-Aldrich).
Antibacterial activity of HAP/PVA/CS/Gent coating was evaluated against Staphylococcus aureus TL (culture collection-
FTM, University of Belgrade, Serbia) and Escherichia coli ATCC 25922, while kinetics of antibacterial activity was monitored
according to our previously published data [1]. In vitro cytotoxicity assay was performed towards two fibroblast cell lines
of different origin-mouse origin cell line (L929 (ATCC CRL-6364)) and human lung origin cell line (MRC-5 (ATCC CCL-171)) [2].
To evaluate the statistical significance of the biological assay results (antibacterial activity, cytotoxicity and ALP activity),
one-way analysis of variance (ANOVA) followed by a multiple comparisons posthoc test was used.
RESULTS AND DISCUSSION: HAP/PVA/CS/Gent coating exhibited strong antibacterial effect against both S. aureus
and E. coli, especially pronounced against S. aureus, causing bactericidal effect. Cytotoxic effect of HAP/PVA/CS/Gent
coating was not pronounced in investigated MRC-5 and L929 cell lines. MRC-5 fibroblast cells in contact with
HAP/PVA/CS/Gent doubled alkaline phosphatase levels compared to their contact with the control samples
(HAP/PVA/CS), indicating good osteogenic properties.
CONCLUSIONS: Electrophoretically deposited HAP/PVA/CS/Gent bioceramic coatings on titanium, demonstrated
significant potential as implants in orthopedic practice, functioning as drug carriers. Not only do they possess
antibacterial properties, but they also exhibit no adverse effects on living tissue.",
publisher = "Beograd : Savez hemijskih inženjera Srbije",
journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices",
title = "Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application",
pages = "59-59",
number = "1s",
volume = "78"
}

Vukašinović-Sekulić, M., Đošić, M., Janković, A., Stevanović, M., Grujić, S., Matić-Bujagić, I., Kojić, V.,& Mišković-Stanković, V.. (2024). Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
Beograd : Savez hemijskih inženjera Srbije., 78(1s), 59-59.

Vukašinović-Sekulić M, Đošić M, Janković A, Stevanović M, Grujić S, Matić-Bujagić I, Kojić V, Mišković-Stanković V. Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):59-59..

Vukašinović-Sekulić, Maja, Đošić, Marija, Janković, Ana, Stevanović, Milena, Grujić, Svetlana, Matić-Bujagić, Ivana, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):59-59.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Sprecher, Christoph Martin
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1243
AB  - INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.
PB  - Beograd : Savez hemijskih inženjera Srbije
C3  - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
T1  - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
EP  - 58
IS  - 1s
SP  - 58
VL  - 78
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2024",
abstract = "INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.",
publisher = "Beograd : Savez hemijskih inženjera Srbije",
journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices",
title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach",
pages = "58-58",
number = "1s",
volume = "78"
}

Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
Beograd : Savez hemijskih inženjera Srbije., 78(1s), 58-58.

Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):58-58..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):58-58.

TY  - CONF
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Stevanović, Milena
AU  - Vukašinović-Sekulić, Vesna
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1242
AB  - INTRODUCTION: Electrochemical methods were employed for synthesizing composites intended for applications in
medicine as antibacterial coatings on titanium bone implants or as highly efficient antibacterial hydrogels for
accelerated wound healing.
EXPERIMENTAL: Hydroxyapatite-based coatings were single-step electrophoretically deposited from fourcomponent
aqueous suspension containing 1 wt% hydroxyapatite powder (HAP, particles < 200 nm particle size, Sigma-
Aldrich), 0.1 wt% poly(vinyl alcohol) (PVA, medium molecular weight, Sigma-Aldrich), 0.05 wt% chitosan powder (CS,
medium molecular weight, Sigma-Aldrich), and aqueous gentamicin sulfate solution (Gent, concentration 50 mg/ml,
Sigma-Aldrich) on titanium plates (Sigma-Aldrich). Antibacterial activity of HAP/PVA/CS/Gent coating was evaluated
against Staphylococcus aureus TL (culture collection-FTM, University of Belgrade, Serbia) and Escherichia coli ATCC
25922. For poly(vinyl alcohol)/chitosan (PVA/CHI) hydrogel synthesis poly(vinyl alcohol) powder (PVA, Sigma Aldrich,
USA), chitosan powder (CHI, Sigma Aldrich, USA), graphene (Graphene Supermarket, USA), glacial acetic acid (Beta
Hem), silver nitrate (MP Hemija), potassium nitrate (Centrohem, Serbia) were used. For antibacterial properties
evaluation, monobasic and dibasic (Sigma Aldrich, USA) potassium phosphates were used. Cell culture suspensions for
cytotoxicity tests were prepared using MTT tetrazolium salt, EDTA, fetal calf serum and antibiotic-antimycotic solution
(Sigma Aldrich, USA).
RESULTS AND DISCUSSION: Surface modification of titanium with innovative bioactive coatings enhances
biomineralization and reduces post-operation pain and infection risk. Using powerful single-step electrophoretic
deposition (EPD) at a constant voltage we have produced various hydroxyapatite-based composite bioceramic coatings
in combination with polymers (lignin, chitosan), graphene, and antibacterial agents (silver, gentamicin).
Electrochemical methods enable in situ synthesis of AgNPs inside polymer matrices, with the main advantage being
the complete absence of any chemical reducing agents that are often toxic and difficult to remove from the material.
The electrochemical reduction of Ag+ ions is achieved only using electrical current or pure hydrogen gas that is generated
at the cathode in aqueous electrolytes, which allows for obtaining completely green and non-toxic product.
Modifications in the synthesis process also enable the control of AgNPs properties, such as size and concentration.
CONCLUSIONS: Composite antibacterial coatings were obtained on titanium plates using EPD. Strong antibacterial
activity against E. coli and S. aureus was found. Biocompatibility was confirmed using in vitro MTT testing since a noncytotoxic
effect was shown towards healthy human peripheral blood mononuclear cells PBMC, fibroblast cell lines MRC-5
and L929, suggesting high potential for bone tissue engineering and medical applications. Hydrogels composed of physically
cross-linked poly(vinyl alcohol)/chitosan with embedded AgNPs were successfully developed for applications as antibacterial
wound dressings due to their highly favorable properties of silver release, antibacterial activity, and non-toxicity.
PB  - Beograd : Savez hemijskih inženjera Srbije
C3  - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
T1  - Electrochemically synthesized biomaterials
EP  - 57
IS  - 1s
SP  - 57
VL  - 78
ER  - 

@conference{
author = "Janković, Ana and Đošić, Marija and Stevanović, Milena and Vukašinović-Sekulić, Vesna and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2024",
abstract = "INTRODUCTION: Electrochemical methods were employed for synthesizing composites intended for applications in
medicine as antibacterial coatings on titanium bone implants or as highly efficient antibacterial hydrogels for
accelerated wound healing.
EXPERIMENTAL: Hydroxyapatite-based coatings were single-step electrophoretically deposited from fourcomponent
aqueous suspension containing 1 wt% hydroxyapatite powder (HAP, particles < 200 nm particle size, Sigma-
Aldrich), 0.1 wt% poly(vinyl alcohol) (PVA, medium molecular weight, Sigma-Aldrich), 0.05 wt% chitosan powder (CS,
medium molecular weight, Sigma-Aldrich), and aqueous gentamicin sulfate solution (Gent, concentration 50 mg/ml,
Sigma-Aldrich) on titanium plates (Sigma-Aldrich). Antibacterial activity of HAP/PVA/CS/Gent coating was evaluated
against Staphylococcus aureus TL (culture collection-FTM, University of Belgrade, Serbia) and Escherichia coli ATCC
25922. For poly(vinyl alcohol)/chitosan (PVA/CHI) hydrogel synthesis poly(vinyl alcohol) powder (PVA, Sigma Aldrich,
USA), chitosan powder (CHI, Sigma Aldrich, USA), graphene (Graphene Supermarket, USA), glacial acetic acid (Beta
Hem), silver nitrate (MP Hemija), potassium nitrate (Centrohem, Serbia) were used. For antibacterial properties
evaluation, monobasic and dibasic (Sigma Aldrich, USA) potassium phosphates were used. Cell culture suspensions for
cytotoxicity tests were prepared using MTT tetrazolium salt, EDTA, fetal calf serum and antibiotic-antimycotic solution
(Sigma Aldrich, USA).
RESULTS AND DISCUSSION: Surface modification of titanium with innovative bioactive coatings enhances
biomineralization and reduces post-operation pain and infection risk. Using powerful single-step electrophoretic
deposition (EPD) at a constant voltage we have produced various hydroxyapatite-based composite bioceramic coatings
in combination with polymers (lignin, chitosan), graphene, and antibacterial agents (silver, gentamicin).
Electrochemical methods enable in situ synthesis of AgNPs inside polymer matrices, with the main advantage being
the complete absence of any chemical reducing agents that are often toxic and difficult to remove from the material.
The electrochemical reduction of Ag+ ions is achieved only using electrical current or pure hydrogen gas that is generated
at the cathode in aqueous electrolytes, which allows for obtaining completely green and non-toxic product.
Modifications in the synthesis process also enable the control of AgNPs properties, such as size and concentration.
CONCLUSIONS: Composite antibacterial coatings were obtained on titanium plates using EPD. Strong antibacterial
activity against E. coli and S. aureus was found. Biocompatibility was confirmed using in vitro MTT testing since a noncytotoxic
effect was shown towards healthy human peripheral blood mononuclear cells PBMC, fibroblast cell lines MRC-5
and L929, suggesting high potential for bone tissue engineering and medical applications. Hydrogels composed of physically
cross-linked poly(vinyl alcohol)/chitosan with embedded AgNPs were successfully developed for applications as antibacterial
wound dressings due to their highly favorable properties of silver release, antibacterial activity, and non-toxicity.",
publisher = "Beograd : Savez hemijskih inženjera Srbije",
journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices",
title = "Electrochemically synthesized biomaterials",
pages = "57-57",
number = "1s",
volume = "78"
}

Janković, A., Đošić, M., Stevanović, M., Vukašinović-Sekulić, V., Kojić, V.,& Mišković-Stanković, V.. (2024). Electrochemically synthesized biomaterials. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
Beograd : Savez hemijskih inženjera Srbije., 78(1s), 57-57.

Janković A, Đošić M, Stevanović M, Vukašinović-Sekulić V, Kojić V, Mišković-Stanković V. Electrochemically synthesized biomaterials. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):57-57..

Janković, Ana, Đošić, Marija, Stevanović, Milena, Vukašinović-Sekulić, Vesna, Kojić, Vesna, Mišković-Stanković, Vesna, "Electrochemically synthesized biomaterials" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):57-57.

TY  - JOUR
AU  - Mišković-Stanković, Vesna
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Radojević, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Đošić, Marija
AU  - Atanasković, Teodor
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1229
AB  - This study presents comparison of our recently formulated two compartmental model with General fractional derivative (GFD) and Korsmeyer-Peppas, Makoid-Banakar and Kopcha diffusion models. We have used our GFD
model to study the release of gentamicin in poly (vinylalcohol)/chitosan/gentamicin (PVA/CHI/Gent) hydrogel aimed for wound dressing in medical treatment of deep chronical wounds. The PVA/CHI/Gent hydrogel was prepared by physical cross linking of poly(vinyl alcohol)/chitosan dispersion using freezing-thawing method, and then was swollen for 48 h in gentamicin solution, at 37 °C. Different physico-chemical (FTIR, SEM), mechanical and biological (cytotoxicity, antibacterial activity) properties have been determined. The concentration of released gentamicin was determined
using a high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS). The ratio between concentration of released gentamicin and initial concentration of gentamicin in the hydrogel was monitored for the
prolonged time period in order to obtain gentamicin release profile. It was proven that our novel diffusion GFD model better fitted with experimental data, and enabled the determination of diffusion coefficient precisely for the entire time period.
PB  - Belgrade :  Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel
DO  - 10.2298/JSC231207010M
ER  - 

@article{
author = "Mišković-Stanković, Vesna and Janković, Ana and Grujić, Svetlana and Matić-Bujagić, Ivana and Radojević, Vesna and Vukašinović-Sekulić, Maja and Kojić, Vesna and Đošić, Marija and Atanasković, Teodor",
year = "2024",
abstract = "This study presents comparison of our recently formulated two compartmental model with General fractional derivative (GFD) and Korsmeyer-Peppas, Makoid-Banakar and Kopcha diffusion models. We have used our GFD
model to study the release of gentamicin in poly (vinylalcohol)/chitosan/gentamicin (PVA/CHI/Gent) hydrogel aimed for wound dressing in medical treatment of deep chronical wounds. The PVA/CHI/Gent hydrogel was prepared by physical cross linking of poly(vinyl alcohol)/chitosan dispersion using freezing-thawing method, and then was swollen for 48 h in gentamicin solution, at 37 °C. Different physico-chemical (FTIR, SEM), mechanical and biological (cytotoxicity, antibacterial activity) properties have been determined. The concentration of released gentamicin was determined
using a high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS). The ratio between concentration of released gentamicin and initial concentration of gentamicin in the hydrogel was monitored for the
prolonged time period in order to obtain gentamicin release profile. It was proven that our novel diffusion GFD model better fitted with experimental data, and enabled the determination of diffusion coefficient precisely for the entire time period.",
publisher = "Belgrade :  Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel",
doi = "10.2298/JSC231207010M"
}

Mišković-Stanković, V., Janković, A., Grujić, S., Matić-Bujagić, I., Radojević, V., Vukašinović-Sekulić, M., Kojić, V., Đošić, M.,& Atanasković, T.. (2024). Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel. in Journal of the Serbian Chemical Society
Belgrade :  Serbian Chemical Society..
https://doi.org/10.2298/JSC231207010M

Mišković-Stanković V, Janković A, Grujić S, Matić-Bujagić I, Radojević V, Vukašinović-Sekulić M, Kojić V, Đošić M, Atanasković T. Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel. in Journal of the Serbian Chemical Society. 2024;.
doi:10.2298/JSC231207010M .

Mišković-Stanković, Vesna, Janković, Ana, Grujić, Svetlana, Matić-Bujagić, Ivana, Radojević, Vesna, Vukašinović-Sekulić, Maja, Kojić, Vesna, Đošić, Marija, Atanasković, Teodor, "Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel" in Journal of the Serbian Chemical Society (2024),
https://doi.org/10.2298/JSC231207010M . .

TY  - JOUR
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1228
AB  - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using  the  constant voltage  method. Characterization of  the  deposited coatings  was performed by scanning electron  micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion  (XRD)  after  immersion  in  simulated  body  fluid  (SBF)  at  37°C.  The  concentration  of  the  released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
PB  - Skopje : Society of Chemists and Technologists of Macedonia
PB  - Skopje : Ss. Cyril and Methodius University in Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications
EP  - 262
IS  - 2
SP  - 249
VL  - 42
DO  - 10.20450/mjcce.2023.2775
ER  - 

@article{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić-Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using  the  constant voltage  method. Characterization of  the  deposited coatings  was performed by scanning electron  micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion  (XRD)  after  immersion  in  simulated  body  fluid  (SBF)  at  37°C.  The  concentration  of  the  released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.",
publisher = "Skopje : Society of Chemists and Technologists of Macedonia, Skopje : Ss. Cyril and Methodius University in Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications",
pages = "262-249",
number = "2",
volume = "42",
doi = "10.20450/mjcce.2023.2775"
}

Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić-Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering
Skopje : Society of Chemists and Technologists of Macedonia., 42(2), 249-262.
https://doi.org/10.20450/mjcce.2023.2775

Jaćimović N, Đošić M, Janković A, Grujić S, Matić-Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262.
doi:10.20450/mjcce.2023.2775 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić-Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262,
https://doi.org/10.20450/mjcce.2023.2775 . .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/666
AB  - Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants.
Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin
(Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral
composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with
improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration
of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent
coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric
analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP.
The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating
against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5
and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent)
expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was
investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent
to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents,
along with antibiotic gentamicin, improving the antibacterial coatings’ properties.
PB  - Elsevier
T2  - Materials Chemistry and Physics
T1  - Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants
IS  - 127766
VL  - 303
DO  - 10.1016/j.matchemphys.2023.127766
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants.
Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin
(Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral
composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with
improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration
of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent
coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric
analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP.
The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating
against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5
and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent)
expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was
investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent
to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents,
along with antibiotic gentamicin, improving the antibacterial coatings’ properties.",
publisher = "Elsevier",
journal = "Materials Chemistry and Physics",
title = "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants",
number = "127766",
volume = "303",
doi = "10.1016/j.matchemphys.2023.127766"
}

Đošić, M., Janković, A., Stevanović, M., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics
Elsevier., 303(127766).
https://doi.org/10.1016/j.matchemphys.2023.127766

Đošić M, Janković A, Stevanović M, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics. 2023;303(127766).
doi:10.1016/j.matchemphys.2023.127766 .

Đošić, Marija, Janković, Ana, Stevanović, Milena, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants" in Materials Chemistry and Physics, 303, no. 127766 (2023),
https://doi.org/10.1016/j.matchemphys.2023.127766 . .

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/750
AB  - Kompozitna prevlaka hidroksiapatita (HAP) sa prirodnim polimerima hitozanom (CS) i polivinil-alkoholom (PVA), kao i sa antibiotikom gentamicinom (Gent) je elektroforetski taložena na titanu, pri konstantnom naponu. Formiranje nove kompozitne HAP/PVA/CS/Gent prevlake je potvrđeno infracrvenom spektroskopijom sa Furijeovom transformacijom. Necitotoksični efekat istaložene HAP/PVA/CS/Gent prevlake je dokazan MTT testom prema dve ćelijske linije (MRC-5 i L929). Sposobnost HAP/PVA/CS/Gent prevlake da indukuje i promoviše osteointegraciju je pokazana ALP testom.
AB  - Composite coating of hydroxyapatite (HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol) (PVA) with the antibiotic gentamicin (Gent) was electrophoretically deposited on titanium from an aqueous suspension, at a constant voltage. Formation of a new composite HAP/PVA/CS/Gent coating was confirmed by Fourier transform infrared spectroscopy. Non-cytotoxicity of deposited HAP/PVA/CS/Gent coating was demonstrated by MTT assay towards two types of cell lines (MRC-5 and L929). HAP/PVA/CS/Gent coating ability to induce and promote the osseointegration process was proved by the ALP assay.
PB  - Belgrade : Serbian Chemical Society
C3  - 59. Savetovanje Srpskog hemijskog društva
T1  - Bioaktivne kompozitne prevlake na bazi hidroksiapatita na titanu za primene u ortopediji / Bioactive hydroxyapatite-based composite coatings on titanium for orthopedic applications
EP  - 64
SP  - 64
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Kompozitna prevlaka hidroksiapatita (HAP) sa prirodnim polimerima hitozanom (CS) i polivinil-alkoholom (PVA), kao i sa antibiotikom gentamicinom (Gent) je elektroforetski taložena na titanu, pri konstantnom naponu. Formiranje nove kompozitne HAP/PVA/CS/Gent prevlake je potvrđeno infracrvenom spektroskopijom sa Furijeovom transformacijom. Necitotoksični efekat istaložene HAP/PVA/CS/Gent prevlake je dokazan MTT testom prema dve ćelijske linije (MRC-5 i L929). Sposobnost HAP/PVA/CS/Gent prevlake da indukuje i promoviše osteointegraciju je pokazana ALP testom., Composite coating of hydroxyapatite (HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol) (PVA) with the antibiotic gentamicin (Gent) was electrophoretically deposited on titanium from an aqueous suspension, at a constant voltage. Formation of a new composite HAP/PVA/CS/Gent coating was confirmed by Fourier transform infrared spectroscopy. Non-cytotoxicity of deposited HAP/PVA/CS/Gent coating was demonstrated by MTT assay towards two types of cell lines (MRC-5 and L929). HAP/PVA/CS/Gent coating ability to induce and promote the osseointegration process was proved by the ALP assay.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59. Savetovanje Srpskog hemijskog društva",
title = "Bioaktivne kompozitne prevlake na bazi hidroksiapatita na titanu za primene u ortopediji / Bioactive hydroxyapatite-based composite coatings on titanium for orthopedic applications",
pages = "64-64"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V.,& Mišković-Stanković, V.. (2023). Bioaktivne kompozitne prevlake na bazi hidroksiapatita na titanu za primene u ortopediji / Bioactive hydroxyapatite-based composite coatings on titanium for orthopedic applications. in 59. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 64-64.

Stevanović M, Đošić M, Janković A, Kojić V, Mišković-Stanković V. Bioaktivne kompozitne prevlake na bazi hidroksiapatita na titanu za primene u ortopediji / Bioactive hydroxyapatite-based composite coatings on titanium for orthopedic applications. in 59. Savetovanje Srpskog hemijskog društva. 2023;:64-64..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioaktivne kompozitne prevlake na bazi hidroksiapatita na titanu za primene u ortopediji / Bioactive hydroxyapatite-based composite coatings on titanium for orthopedic applications" in 59. Savetovanje Srpskog hemijskog društva (2023):64-64.

TY  - CONF
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/749
AB  - Uvođenje antibiotika u polimer/mineralnu kompozitnu prevlaku poboljšava antibakterijska svojstva prevlaka. Kompozitna prevlaka (hidroksiapatit - HAP, poli(vinilalkohol), hitozan, gentamicin) dobijena je elektroforetskim taloženjem iz četvorokomponentne vodene suspenzije. Primenom termogravimetrijske i rendgenske difrakcione analize pokazano je prisustvo karbonatno-supstituisanog HAP. Antibakterijska aktivnost kompozitne prevlake sa gentamicinom potvrđena je protiv soja S. aureus i E. coli, čineći ovu prevlaku pogodnom za potencijalnu primenu u biomedicini.
AB  - Antibiotic introduction in polymer/mineral coating can improve the antibacterial properties of coatings. Composite coating (hydroxyapatite, poly(vinyl alcohol), chitosan, gentamicin) was fabricated from a four-component aqueous suspension using electrophoretic deposition. Structural and morphological characterization of the coating was investigated by X-ray diffraction and thermogravimetric analysis proving carbonate-substituted HAP. The presence of gentamicin contributed to the antibacterial activity of composite coating against S. aureus and E. coli strains, representing a suitable material for possible application in biomedicine.
PB  - Belgrade : Serbian Chemical Society
C3  - 59. Savetovanje Srpskog hemijskog društva
T1  - Elektroforetski taložene kompozitne prevlake sa gentamicinom
T1  - Electrophoretically deposited gentamicin-loaded composite coating
EP  - 58
SP  - 58
ER  - 

@conference{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Uvođenje antibiotika u polimer/mineralnu kompozitnu prevlaku poboljšava antibakterijska svojstva prevlaka. Kompozitna prevlaka (hidroksiapatit - HAP, poli(vinilalkohol), hitozan, gentamicin) dobijena je elektroforetskim taloženjem iz četvorokomponentne vodene suspenzije. Primenom termogravimetrijske i rendgenske difrakcione analize pokazano je prisustvo karbonatno-supstituisanog HAP. Antibakterijska aktivnost kompozitne prevlake sa gentamicinom potvrđena je protiv soja S. aureus i E. coli, čineći ovu prevlaku pogodnom za potencijalnu primenu u biomedicini., Antibiotic introduction in polymer/mineral coating can improve the antibacterial properties of coatings. Composite coating (hydroxyapatite, poly(vinyl alcohol), chitosan, gentamicin) was fabricated from a four-component aqueous suspension using electrophoretic deposition. Structural and morphological characterization of the coating was investigated by X-ray diffraction and thermogravimetric analysis proving carbonate-substituted HAP. The presence of gentamicin contributed to the antibacterial activity of composite coating against S. aureus and E. coli strains, representing a suitable material for possible application in biomedicine.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59. Savetovanje Srpskog hemijskog društva",
title = "Elektroforetski taložene kompozitne prevlake sa gentamicinom, Electrophoretically deposited gentamicin-loaded composite coating",
pages = "58-58"
}

Đošić, M., Janković, A., Stevanović, M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2023). Elektroforetski taložene kompozitne prevlake sa gentamicinom. in 59. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 58-58.

Đošić M, Janković A, Stevanović M, Vukašinović-Sekulić M, Mišković-Stanković V. Elektroforetski taložene kompozitne prevlake sa gentamicinom. in 59. Savetovanje Srpskog hemijskog društva. 2023;:58-58..

Đošić, Marija, Janković, Ana, Stevanović, Milena, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektroforetski taložene kompozitne prevlake sa gentamicinom" in 59. Savetovanje Srpskog hemijskog društva (2023):58-58.

TY  - CONF
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/753
AB  - Multifunkcionalni i višekomponentni biomaterijali se cesto primenjuju u rekonstruktivnoj
hirurgiji zbog sposobnosti da pospešuju osteointegraciju, istovremeno omogucavajuci
primenu antibakterijskih agenasa direktno na mestu intervencije. Primenom
elektroforetskog taloženja, iz cetvorokomponentne vodene suspenzije (hidroksiapatit,
poli(vinilalkohol), hitozan i gentamicin) dobijene su, u jednom koraku, bioaktivne i
antibakterijske kompozitne prevlake na titanu. Karakterizacija prevlaka je izvršena
primenom rendgenske difrakcione analize (XRD), infracrvene spektroskopije sa
Furijeovom transformacijom (FTIR) i skenirajuce elektronske mikroskopije (SEM).
Pracenjem kinetike antibakterijske aktivnosti u suspenziji potvr ena je antibakterijska
efikasnost prevlake sa gentamicinom. Prema dobijenim rezultatima moguce je zakljuciti da
dobijene kompozitne prevlake sa gentamicinom predstavljaju uspešan biomaterijal za
potencijalnu primenu u biomedicini.
AB  - Multifunctional and multicomponent biomaterials are very often the material of choice in
reconstructive surgery, having the ability to promote osteointegration, allowing the
antibacterial agents application directly at the site of intervention. Single-step
electrophoretic deposition was employed to assemble bioactive and antibacterial composite
coatings on titanium, from a four-component aqueous suspension (hydroxyapatite,
poly(vinyl alcohol), chitosan and gentamicin). Physico-chemical characterization of the
coatings was performed using X-ray diffraction (XRD), Fourier transform infrared
spectroscopy (FTIR) and scanning electron microscopy (SEM). Antibacterial activity of
gentamicin loaded coating was confirmed by antibacterial activity kinetics in suspension.
According to the obtained results, it can be concluded that composite coating loaded with
gentamicin represents a successful biomaterial for possible application in biomedicine.
PB  - Belgrade : Serbian Chemical Society
C3  - 58. Savetovanje Srpskog hemijskog društva
T1  - Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium
EP  - 71
SP  - 71
ER  - 

@conference{
author = "Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Multifunkcionalni i višekomponentni biomaterijali se cesto primenjuju u rekonstruktivnoj
hirurgiji zbog sposobnosti da pospešuju osteointegraciju, istovremeno omogucavajuci
primenu antibakterijskih agenasa direktno na mestu intervencije. Primenom
elektroforetskog taloženja, iz cetvorokomponentne vodene suspenzije (hidroksiapatit,
poli(vinilalkohol), hitozan i gentamicin) dobijene su, u jednom koraku, bioaktivne i
antibakterijske kompozitne prevlake na titanu. Karakterizacija prevlaka je izvršena
primenom rendgenske difrakcione analize (XRD), infracrvene spektroskopije sa
Furijeovom transformacijom (FTIR) i skenirajuce elektronske mikroskopije (SEM).
Pracenjem kinetike antibakterijske aktivnosti u suspenziji potvr ena je antibakterijska
efikasnost prevlake sa gentamicinom. Prema dobijenim rezultatima moguce je zakljuciti da
dobijene kompozitne prevlake sa gentamicinom predstavljaju uspešan biomaterijal za
potencijalnu primenu u biomedicini., Multifunctional and multicomponent biomaterials are very often the material of choice in
reconstructive surgery, having the ability to promote osteointegration, allowing the
antibacterial agents application directly at the site of intervention. Single-step
electrophoretic deposition was employed to assemble bioactive and antibacterial composite
coatings on titanium, from a four-component aqueous suspension (hydroxyapatite,
poly(vinyl alcohol), chitosan and gentamicin). Physico-chemical characterization of the
coatings was performed using X-ray diffraction (XRD), Fourier transform infrared
spectroscopy (FTIR) and scanning electron microscopy (SEM). Antibacterial activity of
gentamicin loaded coating was confirmed by antibacterial activity kinetics in suspension.
According to the obtained results, it can be concluded that composite coating loaded with
gentamicin represents a successful biomaterial for possible application in biomedicine.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "58. Savetovanje Srpskog hemijskog društva",
title = "Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium",
pages = "71-71"
}

Đošić, M., Janković, A., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2022). Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium. in 58. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 71-71.

Đošić M, Janković A, Vukašinović-Sekulić M, Mišković-Stanković V. Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium. in 58. Savetovanje Srpskog hemijskog društva. 2022;:71-71..

Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium" in 58. Savetovanje Srpskog hemijskog društva (2022):71-71.

TY  - CONF
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/751
AB  - Titanium surface was modified by applying new composite, biocompatible, and bioactive hydroxyapatite/chitosan coatings with and without gentamicin using the electrophoretic deposition technique (HAP/CS, HAP/CS/Gent, HAP/CS/Gr, and HAP/CS/Gr/Gent). HAP/CS/Gent and HAP/CS/Gr/Gent coatings presented strong activity against both bacterial strains tested and even noticeably more against S. aureus, while their counterparts without the antibiotic did not exhibit antibacterial properties. The rapid and strong antibacterial effect monitored during the first 24 h coincides well with the initial burst effect of gentamicin observed on the cumulative release profiles. Presented results are a solid confirmation that bioceramic HAP/CS/Gent and HAP/CS/Gr/Gent composite coatings electrodeposited on Ti are promising biomaterials for further investigation and potential biomedical application as orthopedic and dental implants
PB  - Roma : Consiglio Nazionale delle Ricerche Istituto di Struttura della Materia
C3  - 3rd  BIENNIAL  CONFERENCE BIOMATERIALS AND NOVEL TECHNOLOGIES FOR HEALTHCARE - BIOMAH 2022
T1  - Bioactive antibiotic-eluting hydroxyapatite-based coatings obtained by electrophoretic deposition on titanium
EP  - 218
SP  - 216
ER  - 

@conference{
author = "Janković, Ana and Đošić, Marija and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Titanium surface was modified by applying new composite, biocompatible, and bioactive hydroxyapatite/chitosan coatings with and without gentamicin using the electrophoretic deposition technique (HAP/CS, HAP/CS/Gent, HAP/CS/Gr, and HAP/CS/Gr/Gent). HAP/CS/Gent and HAP/CS/Gr/Gent coatings presented strong activity against both bacterial strains tested and even noticeably more against S. aureus, while their counterparts without the antibiotic did not exhibit antibacterial properties. The rapid and strong antibacterial effect monitored during the first 24 h coincides well with the initial burst effect of gentamicin observed on the cumulative release profiles. Presented results are a solid confirmation that bioceramic HAP/CS/Gent and HAP/CS/Gr/Gent composite coatings electrodeposited on Ti are promising biomaterials for further investigation and potential biomedical application as orthopedic and dental implants",
publisher = "Roma : Consiglio Nazionale delle Ricerche Istituto di Struttura della Materia",
journal = "3rd  BIENNIAL  CONFERENCE BIOMATERIALS AND NOVEL TECHNOLOGIES FOR HEALTHCARE - BIOMAH 2022",
title = "Bioactive antibiotic-eluting hydroxyapatite-based coatings obtained by electrophoretic deposition on titanium",
pages = "218-216"
}

Janković, A., Đošić, M.,& Mišković-Stanković, V.. (2022). Bioactive antibiotic-eluting hydroxyapatite-based coatings obtained by electrophoretic deposition on titanium. in 3rd  BIENNIAL  CONFERENCE BIOMATERIALS AND NOVEL TECHNOLOGIES FOR HEALTHCARE - BIOMAH 2022
Roma : Consiglio Nazionale delle Ricerche Istituto di Struttura della Materia., 216-218.

Janković A, Đošić M, Mišković-Stanković V. Bioactive antibiotic-eluting hydroxyapatite-based coatings obtained by electrophoretic deposition on titanium. in 3rd  BIENNIAL  CONFERENCE BIOMATERIALS AND NOVEL TECHNOLOGIES FOR HEALTHCARE - BIOMAH 2022. 2022;:216-218..

Janković, Ana, Đošić, Marija, Mišković-Stanković, Vesna, "Bioactive antibiotic-eluting hydroxyapatite-based coatings obtained by electrophoretic deposition on titanium" in 3rd  BIENNIAL  CONFERENCE BIOMATERIALS AND NOVEL TECHNOLOGIES FOR HEALTHCARE - BIOMAH 2022 (2022):216-218.

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/752
AB  - Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers Conference – Materials Science and Engineering
T1  - Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium
EP  - 15
SP  - 15
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers Conference – Materials Science and Engineering",
title = "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium",
pages = "15-15"
}

Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2022). Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 15-15.

Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering. 2022;:15-15..

Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium" in Twentieth Young Researchers Conference – Materials Science and Engineering (2022):15-15.

TY  - CONF
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Jaćimović, Nevena
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/754
AB  - Metoda elektroforetskog taloženja (EPD)se koristi za sintezu biokompatibilnih
kompozitnih materijala za medicinsku primenu kao antibakterijske prevlake za koštane
implantate. Taloženjem pri konstantnom naponu je uspešno dobijena kompozitna prevlaka
hidroksiapatit/hitozan/poli(vinilalkohol) (HAP/CS/PVA) koja sadrži gentamicin (Gent).
Bioaktivnost prevlake je dokazana formiranjem novonastalog apatitnog sloja u simuliranoj
telesnoj tecnosti i karakterisana primenom skenirajuce elektronske mikroskopije (SEM) I
infracrvene spektroskopije sa Furijeovom transformacijom (FTIR). Sposobnost
oseointegracije dalje je potvr ena merenjem aktivnosti alkalne fosfataze (ALP), što
ukazuje na povoljna oseopromotivna svojstva taloženih prevlaka. Kada su testirane
humane i mišje fibroblastne celijske linije (MRC-5 i L929), kompozitne prevlake nisu
pokazale citotoksicni efekat
AB  - The electrophoretic deposition (EPD) method isused for the synthesis of biocompatible
composite materials for medical use as antibacterial coatings for bone implants. Deposition
at the constant voltage yielded composite coating hydroxyapatite/chitosan/poly(vinyl
alcohol)(HAP/CS/PVA) loaded with gentamicin (Gent). Bioactivity of the coating was
proved by a newly formed apatite layer in simulated body fluid and characterized
usingscanning electron microscopy (SEM) and Fourier transform infrared spectroscopy
(FTIR). The osseointegration ability was validated by measuring the alkaline phosphatase
activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite
coatings did not exhibit cytotoxic effect.
PB  - Belgrade : Serbian Chemical Society
C3  - 58. Savetovanje Srpskog hemijskog društva
T1  - Elektroforetski taložene kompozitne prevlake kao nosaci lekova / Electrophoretically deposited composite coatings as drug carriers
EP  - 72
SP  - 72
ER  - 

@conference{
author = "Janković, Ana and Đošić, Marija and Jaćimović, Nevena and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Metoda elektroforetskog taloženja (EPD)se koristi za sintezu biokompatibilnih
kompozitnih materijala za medicinsku primenu kao antibakterijske prevlake za koštane
implantate. Taloženjem pri konstantnom naponu je uspešno dobijena kompozitna prevlaka
hidroksiapatit/hitozan/poli(vinilalkohol) (HAP/CS/PVA) koja sadrži gentamicin (Gent).
Bioaktivnost prevlake je dokazana formiranjem novonastalog apatitnog sloja u simuliranoj
telesnoj tecnosti i karakterisana primenom skenirajuce elektronske mikroskopije (SEM) I
infracrvene spektroskopije sa Furijeovom transformacijom (FTIR). Sposobnost
oseointegracije dalje je potvr ena merenjem aktivnosti alkalne fosfataze (ALP), što
ukazuje na povoljna oseopromotivna svojstva taloženih prevlaka. Kada su testirane
humane i mišje fibroblastne celijske linije (MRC-5 i L929), kompozitne prevlake nisu
pokazale citotoksicni efekat, The electrophoretic deposition (EPD) method isused for the synthesis of biocompatible
composite materials for medical use as antibacterial coatings for bone implants. Deposition
at the constant voltage yielded composite coating hydroxyapatite/chitosan/poly(vinyl
alcohol)(HAP/CS/PVA) loaded with gentamicin (Gent). Bioactivity of the coating was
proved by a newly formed apatite layer in simulated body fluid and characterized
usingscanning electron microscopy (SEM) and Fourier transform infrared spectroscopy
(FTIR). The osseointegration ability was validated by measuring the alkaline phosphatase
activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite
coatings did not exhibit cytotoxic effect.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "58. Savetovanje Srpskog hemijskog društva",
title = "Elektroforetski taložene kompozitne prevlake kao nosaci lekova / Electrophoretically deposited composite coatings as drug carriers",
pages = "72-72"
}

Janković, A., Đošić, M., Jaćimović, N., Kojić, V.,& Mišković-Stanković, V.. (2022). Elektroforetski taložene kompozitne prevlake kao nosaci lekova / Electrophoretically deposited composite coatings as drug carriers. in 58. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 72-72.

Janković A, Đošić M, Jaćimović N, Kojić V, Mišković-Stanković V. Elektroforetski taložene kompozitne prevlake kao nosaci lekova / Electrophoretically deposited composite coatings as drug carriers. in 58. Savetovanje Srpskog hemijskog društva. 2022;:72-72..

Janković, Ana, Đošić, Marija, Jaćimović, Nevena, Kojić, Vesna, Mišković-Stanković, Vesna, "Elektroforetski taložene kompozitne prevlake kao nosaci lekova / Electrophoretically deposited composite coatings as drug carriers" in 58. Savetovanje Srpskog hemijskog društva (2022):72-72.

TY  - JOUR
AU  - Prokić, B.B.
AU  - Božinovski, T.L.
AU  - Gajdov, V.
AU  - Milošević, I.
AU  - Todorović, V.
AU  - Đošić, Marija
AU  - Mišković-Stanković, Vesna
AU  - Marković, D.
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/624
AB  - The ethical dilemmas of using animals as in vivo models in preclinical and clinical examinations have been increasingly present in recent decades. Small laboratory animals (rats, rabbits) will continue to be used because they are cost-effective and permit the formation of statistically testable cohort groups; a task that, for financial, maintenance and care reasons, is almost prohibitive for larger animals. Technological advances in the production of new biomaterials for clinical use are enormous, but screening tests and methods used to assess biocompatibility lag behind these advances. The assessment of biological responses is slow and based on millennial recovery mechanisms in eukaryotic organisms. Therefore, the goal of researchers in this field is to re-evaluate old methods of biocompatibility assessment and introduce new methods of evaluation, especially for in vivo testing. In that sense, a revision of the ISO standards was planned and conducted in 2017, which insisted on cytotoxicity testing in cell lines and produced concrete proposals on how biocompatibility should be quantified. In vivo biocompatibility evaluation of biomaterials used for soft tissue recovery commonly utilises rats. Rabbits are recommended for implants used for hard tissues, because of the rabbit’s size, the possibility of implanting the biomaterials on a larger bone surface, and because of the peculiarities of rabbit bone tissue that favours rapid recovery after bone defects and enables easy reading of the results
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Animal models in bicompatibility assessments of implants in soft and hard tissues
IS  - 1
VL  - 76
DO  - 10.2298/VETGL210322005P
UR  - conv_1002
ER  - 

@article{
author = "Prokić, B.B. and Božinovski, T.L. and Gajdov, V. and Milošević, I. and Todorović, V. and Đošić, Marija and Mišković-Stanković, Vesna and Marković, D.",
year = "2022",
abstract = "The ethical dilemmas of using animals as in vivo models in preclinical and clinical examinations have been increasingly present in recent decades. Small laboratory animals (rats, rabbits) will continue to be used because they are cost-effective and permit the formation of statistically testable cohort groups; a task that, for financial, maintenance and care reasons, is almost prohibitive for larger animals. Technological advances in the production of new biomaterials for clinical use are enormous, but screening tests and methods used to assess biocompatibility lag behind these advances. The assessment of biological responses is slow and based on millennial recovery mechanisms in eukaryotic organisms. Therefore, the goal of researchers in this field is to re-evaluate old methods of biocompatibility assessment and introduce new methods of evaluation, especially for in vivo testing. In that sense, a revision of the ISO standards was planned and conducted in 2017, which insisted on cytotoxicity testing in cell lines and produced concrete proposals on how biocompatibility should be quantified. In vivo biocompatibility evaluation of biomaterials used for soft tissue recovery commonly utilises rats. Rabbits are recommended for implants used for hard tissues, because of the rabbit’s size, the possibility of implanting the biomaterials on a larger bone surface, and because of the peculiarities of rabbit bone tissue that favours rapid recovery after bone defects and enables easy reading of the results",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Animal models in bicompatibility assessments of implants in soft and hard tissues",
number = "1",
volume = "76",
doi = "10.2298/VETGL210322005P",
url = "conv_1002"
}

Prokić, B.B., Božinovski, T.L., Gajdov, V., Milošević, I., Todorović, V., Đošić, M., Mišković-Stanković, V.,& Marković, D.. (2022). Animal models in bicompatibility assessments of implants in soft and hard tissues. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 76(1).
https://doi.org/10.2298/VETGL210322005P
conv_1002

Prokić B, Božinovski T, Gajdov V, Milošević I, Todorović V, Đošić M, Mišković-Stanković V, Marković D. Animal models in bicompatibility assessments of implants in soft and hard tissues. in Veterinarski glasnik. 2022;76(1).
doi:10.2298/VETGL210322005P
conv_1002 .

Prokić, B.B., Božinovski, T.L., Gajdov, V., Milošević, I., Todorović, V., Đošić, Marija, Mišković-Stanković, Vesna, Marković, D., "Animal models in bicompatibility assessments of implants in soft and hard tissues" in Veterinarski glasnik, 76, no. 1 (2022),
https://doi.org/10.2298/VETGL210322005P .,
conv_1002 .

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nesović, Katarina
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/644
PB  - Mary Ann Liebert, Inc, New Rochelle
C3  - Tissue Engineering Part A
T1  - Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations
EP  - S132
SP  - S132
VL  - 28
UR  - conv_946
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nesović, Katarina and Mišković-Stanković, Vesna",
year = "2022",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Tissue Engineering Part A",
title = "Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations",
pages = "S132-S132",
volume = "28",
url = "conv_946"
}

Stevanović, M., Đošić, M., Janković, A., Nesović, K.,& Mišković-Stanković, V.. (2022). Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations. in Tissue Engineering Part A
Mary Ann Liebert, Inc, New Rochelle., 28, S132-S132.
conv_946

Stevanović M, Đošić M, Janković A, Nesović K, Mišković-Stanković V. Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations. in Tissue Engineering Part A. 2022;28:S132-S132.
conv_946 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Nesović, Katarina, Mišković-Stanković, Vesna, "Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations" in Tissue Engineering Part A, 28 (2022):S132-S132,
conv_946 .

Veljović, Đ., Đošić, M., Zvicer, J., Zebić, M., Janaćković, Đ., Janković, A., Matić, T., Miletić, V., Nešović, K., Obradović, B., Osmokrović, A., Petrović, R., Mišković-Stanković, V., Stevanović, M.,& Stojkovska, J.. (2021). New multifunctional biomaterials for medical application. in 35. Međunarodni festival inovacija, znanja i stvaralaštva TESLA FEST 2021
Novi Sad : Savez pronalazača Vojvodine., 5-5.

Veljović Đ, Đošić M, Zvicer J, Zebić M, Janaćković Đ, Janković A, Matić T, Miletić V, Nešović K, Obradović B, Osmokrović A, Petrović R, Mišković-Stanković V, Stevanović M, Stojkovska J. New multifunctional biomaterials for medical application. in 35. Međunarodni festival inovacija, znanja i stvaralaštva TESLA FEST 2021. 2021;:5-5..

Veljović, Đorđe, Đošić, Marija, Zvicer, Jovana, Zebić, Maja, Janaćković, Đorđe, Janković, Ana, Matić, Tamara, Miletić, Vesna, Nešović, Katarina, Obradović, Bojana, Osmokrović, Andrea, Petrović, Rada, Mišković-Stanković, Vesna, Stevanović, Milena, Stojkovska, Jasmina, "New multifunctional biomaterials for medical application" in 35. Međunarodni festival inovacija, znanja i stvaralaštva TESLA FEST 2021 (2021):5-5.

TY  - CONF
AU  - Jaćimović, Nevena
AU  - Mišković-Stanković, Vesna
AU  - Đošić, Marija
AU  - Janković, Ana
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/757
AB  - Uvod: Regenerativna medicina poslednjih decenija postaje jedna od najbrže rastućih oblasti
zdravstva, sa posebnim fokusom na razvoj biomaterijala namenjenih primeni u ortopediji.
Cilj: Optimizacija uslova formiranja biokeramičkih kompozitnih prevlaka iz suspenzije na
bazi hidroksiapatita (HAP), hitozana (CS) i polivinil-alkohola (PVA) sa dodatkom antibiotika
gentamicina (Gent), na titanskom supstratu metodom elekroforetskog taloženja (EPD). Ispitivanje
bioaktivnosti i procena antibakerijskih svojstava prevlake HAP/CS/PVA/Gent.
Materijal i metode: Hidroksiapatit (HAP), hitozan (CS), polivinil-alkohol (PVA) i gentamicin
(Gent) korišćeni su za sintezu suspenzije iz koje su taložene biokeramičke prevlake na titanskim
pločicama (Ti). Za EDP je korišćen instrument Electrophoresis Power Supply EV231 (Peqlab,
Erlangen, Germany). Biokeramičke prevlake na Ti supstratu dobijene su metodom elektroforetskog
taloženja pri konstantnom naponu od 7 V. Pri ovom postupku Ti pločica kao radna katoda postavljena
je između dve platinske (Pt) pločice-anode i elektrode su uronjene u suspenziju HAP/CS/PVA/
Gent (vršena je optimizacija vremena taloženja u rasponu od 5 do 12 min). Radi karakterizacije
dobijenih prevlaka nakon potapanja u simuliranoj telesnoj tečnosti (SBF) na 37°C, nakon perioda
od 7 i 14 dana, korišćena je metoda rendgenske difrakcije (XRD), infracrvena spektroskopija sa
Furijeovom transformacijom (FTIR) i skenirajuća elektronska mikroskopija (SEM). Procena antibakterijske
aktivnosti prema dva bakterijska soja Echerichia coli i Staphylococcus aureus kvalitativno
je određena agar difuzionom metodom.
Rezultati: Dokazana je pretpostavljena bioaktivnost prevlake HAP/CS/PVA/Gent metodama
karakterizacije koje su pokazale rast nove hidroksiapatitne faze, dobru umreženost polimera i
homogenost sintetisane prevlake. Merenjem inhibicionih zona dobijenih agar difuzionom metodom
pokazana je jaka antibakterijska aktivnost prevlake prema oba bakterijska soja.
Zaključak: Biokeramičke prevlake dobijene elektroforetskim taloženjem, iz suspenzije HAP/
CS/PVA/Gent, pokazuju dobru mogućnost primene u regenerativnoj ortopediji.
AB  - Introduction: In recent decades, regenerative medicine has become one of the fastest growing
areas of health care, with an emphasis on developing biomaterials to be used in orthopedics.
Aim: Optimization of deposition conditions of bioceramic composite coatings from suspension
based on hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) with the addition of
gentamicin (Gent), on titanium substrate by electrophoretic deposition process (EPD). Investigation
of coating bioactivity and antibacterial activity.
Material and Methods: Hydroxyapatite (HAP), chitosan (CS), polyvinyl alcohol (PVA) and
gentamicin (Gent) were used to synthesize a suspension from which bioceramic coatings were
deposited on titanium plates (Ti). Electrophoretic deposition was performed using Electrophoresis
Power Supply EV231 (Peqlab, Erlangen, Germany). The bioceramic coatings on the Ti substrate
were obtained by electrophoretic deposition at the constant voltage of 7 V. During this process, Ti
plate was used as a cathode placed between two platinum (Pt) plates – anodes and the electrodes
were immersed in a HAP/CS/PVA/Gent suspension (the deposition time was optimized in the
range from 5 to 12 min). X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR)
and scanning electron microscopy (SEM) methods were employed for the characterization of the
obtained coatings after 7 and 14 days of immersion in a simulated body fluid (SBF) at 37°C. The
antibacterial activity against two bacterial strains Escherichia coli and Staphylococcus aureus was
evaluated using the agar diffusion method.
Results: The assumed bioactivity of the HAP/CS/PVA/Gent coating has been proven by characterization
methods that showed the growth of a new hydroxyapatite phase, good crosslinking
of the polymer and homogeneity of the synthesized coating. Measurement of inhibition zones
obtained by the agar diffusion method showed strong antibacterial activity of the coating against
both bacterial strains.
Conclusion: Bioceramic coatings obtained by electrophoretic deposition from a HAP/CS/PVA/
Gent suspension can be considered for application in regenerative orthopedics.
PB  - Banja Luka : University in Banja Luka
C3  - 14th scientific conference - Students encountering science – StES 2021
T1  - Bioceramic coatings synthesized using electrophoretic deposition
EP  - 60
SP  - 60
ER  - 

@conference{
author = "Jaćimović, Nevena and Mišković-Stanković, Vesna and Đošić, Marija and Janković, Ana",
year = "2021",
abstract = "Uvod: Regenerativna medicina poslednjih decenija postaje jedna od najbrže rastućih oblasti
zdravstva, sa posebnim fokusom na razvoj biomaterijala namenjenih primeni u ortopediji.
Cilj: Optimizacija uslova formiranja biokeramičkih kompozitnih prevlaka iz suspenzije na
bazi hidroksiapatita (HAP), hitozana (CS) i polivinil-alkohola (PVA) sa dodatkom antibiotika
gentamicina (Gent), na titanskom supstratu metodom elekroforetskog taloženja (EPD). Ispitivanje
bioaktivnosti i procena antibakerijskih svojstava prevlake HAP/CS/PVA/Gent.
Materijal i metode: Hidroksiapatit (HAP), hitozan (CS), polivinil-alkohol (PVA) i gentamicin
(Gent) korišćeni su za sintezu suspenzije iz koje su taložene biokeramičke prevlake na titanskim
pločicama (Ti). Za EDP je korišćen instrument Electrophoresis Power Supply EV231 (Peqlab,
Erlangen, Germany). Biokeramičke prevlake na Ti supstratu dobijene su metodom elektroforetskog
taloženja pri konstantnom naponu od 7 V. Pri ovom postupku Ti pločica kao radna katoda postavljena
je između dve platinske (Pt) pločice-anode i elektrode su uronjene u suspenziju HAP/CS/PVA/
Gent (vršena je optimizacija vremena taloženja u rasponu od 5 do 12 min). Radi karakterizacije
dobijenih prevlaka nakon potapanja u simuliranoj telesnoj tečnosti (SBF) na 37°C, nakon perioda
od 7 i 14 dana, korišćena je metoda rendgenske difrakcije (XRD), infracrvena spektroskopija sa
Furijeovom transformacijom (FTIR) i skenirajuća elektronska mikroskopija (SEM). Procena antibakterijske
aktivnosti prema dva bakterijska soja Echerichia coli i Staphylococcus aureus kvalitativno
je određena agar difuzionom metodom.
Rezultati: Dokazana je pretpostavljena bioaktivnost prevlake HAP/CS/PVA/Gent metodama
karakterizacije koje su pokazale rast nove hidroksiapatitne faze, dobru umreženost polimera i
homogenost sintetisane prevlake. Merenjem inhibicionih zona dobijenih agar difuzionom metodom
pokazana je jaka antibakterijska aktivnost prevlake prema oba bakterijska soja.
Zaključak: Biokeramičke prevlake dobijene elektroforetskim taloženjem, iz suspenzije HAP/
CS/PVA/Gent, pokazuju dobru mogućnost primene u regenerativnoj ortopediji., Introduction: In recent decades, regenerative medicine has become one of the fastest growing
areas of health care, with an emphasis on developing biomaterials to be used in orthopedics.
Aim: Optimization of deposition conditions of bioceramic composite coatings from suspension
based on hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) with the addition of
gentamicin (Gent), on titanium substrate by electrophoretic deposition process (EPD). Investigation
of coating bioactivity and antibacterial activity.
Material and Methods: Hydroxyapatite (HAP), chitosan (CS), polyvinyl alcohol (PVA) and
gentamicin (Gent) were used to synthesize a suspension from which bioceramic coatings were
deposited on titanium plates (Ti). Electrophoretic deposition was performed using Electrophoresis
Power Supply EV231 (Peqlab, Erlangen, Germany). The bioceramic coatings on the Ti substrate
were obtained by electrophoretic deposition at the constant voltage of 7 V. During this process, Ti
plate was used as a cathode placed between two platinum (Pt) plates – anodes and the electrodes
were immersed in a HAP/CS/PVA/Gent suspension (the deposition time was optimized in the
range from 5 to 12 min). X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR)
and scanning electron microscopy (SEM) methods were employed for the characterization of the
obtained coatings after 7 and 14 days of immersion in a simulated body fluid (SBF) at 37°C. The
antibacterial activity against two bacterial strains Escherichia coli and Staphylococcus aureus was
evaluated using the agar diffusion method.
Results: The assumed bioactivity of the HAP/CS/PVA/Gent coating has been proven by characterization
methods that showed the growth of a new hydroxyapatite phase, good crosslinking
of the polymer and homogeneity of the synthesized coating. Measurement of inhibition zones
obtained by the agar diffusion method showed strong antibacterial activity of the coating against
both bacterial strains.
Conclusion: Bioceramic coatings obtained by electrophoretic deposition from a HAP/CS/PVA/
Gent suspension can be considered for application in regenerative orthopedics.",
publisher = "Banja Luka : University in Banja Luka",
journal = "14th scientific conference - Students encountering science – StES 2021",
title = "Bioceramic coatings synthesized using electrophoretic deposition",
pages = "60-60"
}

Jaćimović, N., Mišković-Stanković, V., Đošić, M.,& Janković, A.. (2021). Bioceramic coatings synthesized using electrophoretic deposition. in 14th scientific conference - Students encountering science – StES 2021
Banja Luka : University in Banja Luka., 60-60.

Jaćimović N, Mišković-Stanković V, Đošić M, Janković A. Bioceramic coatings synthesized using electrophoretic deposition. in 14th scientific conference - Students encountering science – StES 2021. 2021;:60-60..

Jaćimović, Nevena, Mišković-Stanković, Vesna, Đošić, Marija, Janković, Ana, "Bioceramic coatings synthesized using electrophoretic deposition" in 14th scientific conference - Students encountering science – StES 2021 (2021):60-60.

TY  - CONF
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/759
AB  - Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene.
AB  - Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.
PB  - Belgrade : Serbian Chemical Society
C3  - 57. savetovanje Srpskog hemijskog društva
T1  - Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium
EP  - 46
SP  - 46
ER  - 

@conference{
author = "Janković, Ana and Stevanović, Milena and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Grujić, Svetlana and Matić-Bujagić, Ivana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene., Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "57. savetovanje Srpskog hemijskog društva",
title = "Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium",
pages = "46-46"
}

Janković, A., Stevanović, M., Đošić, M., Vukašinović-Sekulić, M., Kojić, V., Grujić, S., Matić-Bujagić, I.,& Mišković-Stanković, V.. (2021). Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium. in 57. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 46-46.

Janković A, Stevanović M, Đošić M, Vukašinović-Sekulić M, Kojić V, Grujić S, Matić-Bujagić I, Mišković-Stanković V. Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium. in 57. savetovanje Srpskog hemijskog društva. 2021;:46-46..

Janković, Ana, Stevanović, Milena, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Grujić, Svetlana, Matić-Bujagić, Ivana, Mišković-Stanković, Vesna, "Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium" in 57. savetovanje Srpskog hemijskog društva (2021):46-46.

TY  - CONF
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/756
AB  - Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), Xray
diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue."















"Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), Xray
diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Nineteenth Young Researchers’ Conference - Materials Science and Engineering
T1  - Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin
EP  - 7
SP  - 7
ER  - 

@conference{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), Xray
diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue."















"Composite bioceramic coatings have been attracting increasing attention of the scientific
public in recent decades due to the good potential of their use for medical purposes. The
main goal of this scientific research is to show the characterization of the obtained
hydroxyapatite (HAP), chitosan (CS) and polyvinyl alcohol (PVA) bioceramic composite
coatings loaded with gentamicin (Gent), on titanium (Ti) substrate using the single-step
electrophoretic deposition process. Titanium, traditionally used for the purpose of replacing
parts of damaged bone tissue, has shown good mechanical properties and biocompatibility
through many years of orthopedic practice. Hydroxyapatite provided the HAP/CS/PVA/Gent
composite coating with osteoinductivity, while the presence of chitosan and polyvinyl
alcohol contributed to its adhesive and mild analgesic properties. The antibacterial agent
gentamicin was added to the HAP/CS/PVA composite, which ensured its direct antibacterial
effect at the implantation site. The coatings were deposited using the cathodic electrophoretic
deposition process, with the Ti plate as the cathode, at a constant voltage of 7V, in the range
of the deposition time from 5 to 12 min. To demonstrate the presumed bioactivity,
HAP/CS/PVA/Gent coatings were immersed in simulated body fluid (SBF) at 37⁰C for
periods of 7 and 14 days. Characterization of HAP/CS/PVA/Gent coatings after immersion
was performed through employment of Fourier transform infrared spectroscopy (FTIR), Xray
diffraction (XRD), and field emission scanning electron microscopy (SEM) techniques.
Antibacterial activity was tested by agar diffusion method on two bacterial strains,
Echerichia coli and Staphylococcus aureus with a positive response to both bacterial strains.
The final conclusions of this research suggest a good potential for utilization of
HAP/CS/PVA/Gent bioceramic composite coating, applied through electrophoretic
deposition process on Ti substrate, for medical purposes in the replacement and healing of
damaged bone tissue.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Nineteenth Young Researchers’ Conference - Materials Science and Engineering",
title = "Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin",
pages = "7-7"
}

Jaćimović, N., Đošić, M., Janković, A.,& Mišković-Stanković, V.. (2021). Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 7-7.

Jaćimović N, Đošić M, Janković A, Mišković-Stanković V. Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin. in Nineteenth Young Researchers’ Conference - Materials Science and Engineering. 2021;:7-7..

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Electrochemical composite bioceramic coatings based on hydroxyapatite, chitosan and polyvinyl alcohol loaded with gentamicin" in Nineteenth Young Researchers’ Conference - Materials Science and Engineering (2021):7-7.

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/572
AB  - This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.
PB  - Elsevier, Amsterdam
T2  - Journal of Materials Research and Technology-Jmr&T
T1  - The chitosan-based bioactive composite coating on titanium
EP  - 4474
SP  - 4461
VL  - 15
DO  - 10.1016/j.jmrt.2021.10.072
UR  - conv_921
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2021",
abstract = "This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Materials Research and Technology-Jmr&T",
title = "The chitosan-based bioactive composite coating on titanium",
pages = "4474-4461",
volume = "15",
doi = "10.1016/j.jmrt.2021.10.072",
url = "conv_921"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2021). The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology-Jmr&T
Elsevier, Amsterdam., 15, 4461-4474.
https://doi.org/10.1016/j.jmrt.2021.10.072
conv_921

Stevanović M, Đošić M, Janković A, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology-Jmr&T. 2021;15:4461-4474.
doi:10.1016/j.jmrt.2021.10.072
conv_921 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "The chitosan-based bioactive composite coating on titanium" in Journal of Materials Research and Technology-Jmr&T, 15 (2021):4461-4474,
https://doi.org/10.1016/j.jmrt.2021.10.072 .,
conv_921 .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/567
AB  - Current trends in biomaterials science address the issue of integrating artificial materials as orthopedic or dental implants with biological materials, e.g., patients' bone tissue. Problems arise due to the simple fact that any surface that promotes biointegration and facilitates osteointegration may also provide a good platform for the rapid growth of bacterial colonies. Infected implant surfaces easily lead to biofilm formation that poses a major healthcare concern since it could have destructive effects and ultimately endanger the patients' life. As of late, research has centered on designing coatings that would eliminate possible infection but neglected to aid bone mineralization. Other strategies yielded surfaces that could promote osseointegration but failed to prevent microbial susceptibility. Needless to say, in order to assure prolonged implant functionality, both coating functions are indispensable and should be addressed simultaneously. This review summarizes progress in designing multifunctional implant coatings that serve as carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the implant-tissue interface, while still promoting osseointegration.
PB  - MDPI, Basel
T2  - Materials
T1  - Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium
IS  - 18
VL  - 14
DO  - 10.3390/ma14185391
UR  - conv_916
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Current trends in biomaterials science address the issue of integrating artificial materials as orthopedic or dental implants with biological materials, e.g., patients' bone tissue. Problems arise due to the simple fact that any surface that promotes biointegration and facilitates osteointegration may also provide a good platform for the rapid growth of bacterial colonies. Infected implant surfaces easily lead to biofilm formation that poses a major healthcare concern since it could have destructive effects and ultimately endanger the patients' life. As of late, research has centered on designing coatings that would eliminate possible infection but neglected to aid bone mineralization. Other strategies yielded surfaces that could promote osseointegration but failed to prevent microbial susceptibility. Needless to say, in order to assure prolonged implant functionality, both coating functions are indispensable and should be addressed simultaneously. This review summarizes progress in designing multifunctional implant coatings that serve as carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the implant-tissue interface, while still promoting osseointegration.",
publisher = "MDPI, Basel",
journal = "Materials",
title = "Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium",
number = "18",
volume = "14",
doi = "10.3390/ma14185391",
url = "conv_916"
}

Đošić, M., Janković, A.,& Mišković-Stanković, V.. (2021). Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium. in Materials
MDPI, Basel., 14(18).
https://doi.org/10.3390/ma14185391
conv_916

Đošić M, Janković A, Mišković-Stanković V. Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium. in Materials. 2021;14(18).
doi:10.3390/ma14185391
conv_916 .

Đošić, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Electrophoretic Deposition of Biocompatible and Bioactive Hydroxyapatite-Based Coatings on Titanium" in Materials, 14, no. 18 (2021),
https://doi.org/10.3390/ma14185391 .,
conv_916 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/755
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley
T2  - Journal of Biomedical Materials Research
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley",
journal = "Journal of Biomedical Materials Research",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research
Wiley., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Rhee KY, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - vuk, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://www.ise-online.org/ise-conferences/annmeet/folder/70th_Annual_meeting-BoA.pdf
UR  - https://ritnms.itnms.ac.rs/handle/123456789/806
AB  - Bone implant materials are increasingly attracting attention in the biomaterials field. Among them, hydroxyapatite (HAP) has stood out as a prospective biomaterial due to excellent osseointegration ability, owing to its similarity with natural bone. However, due to the lack of adhesive and antibacterial properties, it is usually combined with polymers and antibacterial agents. Natural polymer chitosan (CS) was proven as an effective component of HAP-based composites, improving the adhesion and serving as a drug carrier.1 Inclusion of antibiotics into composite biomaterials has gained a lot of attention, as it is thus possible to achieve the desirable antibacterial activity. Gentamicin (Gent) was shown to be one of the most effective antibiotics in treating bone infections. In this work, bioceramic composite HAP/CS and HAP/CS/Gent coatings were successfully assembled on Ti plates by electrophoretic deposition technique (EPD).2 Deposition was carried out at a constant voltage of 5 V and for 12 min in a single step process from three-component aqueous suspension (Figure 1). Bioactive properties of deposited coatings were investigated in vitro, by immersion in simulated body fluid solution (SBF). After 7-day immersion in SBF, field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) were employed to investigate the coatings’ bioactivity, whereas electrochemical impedance spectroscopy (EIS) and polarization measurements were carried out during 28-day immersion in SBF in order to obtain valuable information about osseopromotive coatings’ nature. Biological testing included monitoring of alkaline phosphatase activity (ALP) that is associated with the coatings’ ability to promote new bone growth. The high increase in ALP activity (50 %) relative to the control pointed to the strong coatings’ aptitude for the osseointegration process and new bone growth. FTIR and FE-SEM analysis gave evidence of carbonate-substituted HAP, whose presence is desirable in bone implant applications. The formation of the new apatite layer was also confirmed by electrochemical measurements pointing to the high biomineralization potential of the coatings. Antibacterial activity kinetics in suspension against Staphylococcus aureus and Escherichia coli was also monitored to examine the potential coatings’ application. As expected, the gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gent coating, and the effect was more pronounced against S. aureus.
All the obtained results potentially indicated a strong osseointegration ability of the HAP/CS/Gent coating classifying it as a promising material for bone tissue engineering.
C3  - 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online
T1  - Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and vuk, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Bone implant materials are increasingly attracting attention in the biomaterials field. Among them, hydroxyapatite (HAP) has stood out as a prospective biomaterial due to excellent osseointegration ability, owing to its similarity with natural bone. However, due to the lack of adhesive and antibacterial properties, it is usually combined with polymers and antibacterial agents. Natural polymer chitosan (CS) was proven as an effective component of HAP-based composites, improving the adhesion and serving as a drug carrier.1 Inclusion of antibiotics into composite biomaterials has gained a lot of attention, as it is thus possible to achieve the desirable antibacterial activity. Gentamicin (Gent) was shown to be one of the most effective antibiotics in treating bone infections. In this work, bioceramic composite HAP/CS and HAP/CS/Gent coatings were successfully assembled on Ti plates by electrophoretic deposition technique (EPD).2 Deposition was carried out at a constant voltage of 5 V and for 12 min in a single step process from three-component aqueous suspension (Figure 1). Bioactive properties of deposited coatings were investigated in vitro, by immersion in simulated body fluid solution (SBF). After 7-day immersion in SBF, field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) were employed to investigate the coatings’ bioactivity, whereas electrochemical impedance spectroscopy (EIS) and polarization measurements were carried out during 28-day immersion in SBF in order to obtain valuable information about osseopromotive coatings’ nature. Biological testing included monitoring of alkaline phosphatase activity (ALP) that is associated with the coatings’ ability to promote new bone growth. The high increase in ALP activity (50 %) relative to the control pointed to the strong coatings’ aptitude for the osseointegration process and new bone growth. FTIR and FE-SEM analysis gave evidence of carbonate-substituted HAP, whose presence is desirable in bone implant applications. The formation of the new apatite layer was also confirmed by electrochemical measurements pointing to the high biomineralization potential of the coatings. Antibacterial activity kinetics in suspension against Staphylococcus aureus and Escherichia coli was also monitored to examine the potential coatings’ application. As expected, the gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gent coating, and the effect was more pronounced against S. aureus.
All the obtained results potentially indicated a strong osseointegration ability of the HAP/CS/Gent coating classifying it as a promising material for bone tissue engineering.",
journal = "71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online",
title = "Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use"
}

Stevanović, M., Đošić, M., Janković, A., vuk, M., Kojić, V.,& Mišković-Stanković, V.. (2020). Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use. in 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online.

Stevanović M, Đošić M, Janković A, vuk M, Kojić V, Mišković-Stanković V. Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use. in 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online. 2020;..

Stevanović, Milena, Đošić, Marija, Janković, Ana, vuk, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use" in 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online (2020).

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nesović, Katarina
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/554
AB  - The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate
EP  - 15445
IS  - 25
SP  - 15433
VL  - 5
DO  - 10.1021/acsomega.0c01583
UR  - conv_884
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nesović, Katarina and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate",
pages = "15445-15433",
number = "25",
volume = "5",
doi = "10.1021/acsomega.0c01583",
url = "conv_884"
}

Stevanović, M., Đošić, M., Janković, A., Nesović, K., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega
Amer Chemical Soc, Washington., 5(25), 15433-15445.
https://doi.org/10.1021/acsomega.0c01583
conv_884

Stevanović M, Đošić M, Janković A, Nesović K, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega. 2020;5(25):15433-15445.
doi:10.1021/acsomega.0c01583
conv_884 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Nesović, Katarina, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate" in ACS Omega, 5, no. 25 (2020):15433-15445,
https://doi.org/10.1021/acsomega.0c01583 .,
conv_884 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukasinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakac, Milkica
AU  - Yop, Rhee Kyong
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/544
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction >3 logarithmic units) compared toEscherichia coli(bacteriostatic, LT 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
UR  - conv_882
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukasinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakac, Milkica and Yop, Rhee Kyong and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction >3 logarithmic units) compared toEscherichia coli(bacteriostatic, LT 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974",
url = "conv_882"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukasinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakac, M., Yop, R. K.,& Mišković-Stanković, V.. (2020). Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974
conv_882

Stevanović M, Đošić M, Janković A, Kojić V, Vukasinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakac M, Yop RK, Mišković-Stanković V. Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974
conv_882 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukasinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakac, Milkica, Yop, Rhee Kyong, Mišković-Stanković, Vesna, "Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 .,
conv_882 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/777
AB  - Electrophoretic deposition (EPD) process was successfully used for
obtaining composite antibacterial coating of antibiotic gentamicin (Gent), natural polymer
chitosan (CS), and hydroxyapatite (HAP). EPD was carried out on pure titanium (Ti) plates in
a single step from three-component aqueous suspension. The gentamicin influence was
examined through detailed physico-chemical and biological characterization. The formation
of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface was confirmed by
X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier
transform infrared spectroscopy (FTIR) and X-ray photoelectron analysis (XPS). Composite
HAP/CS and HAP/CS/Gent coatings exhibited homogeneous, porous fracture-free surface,
suggesting strong interfacial interactions between HAP and CS. Biological assays pointed to
high potential of HAP/CS/Gent coating for medical applications. Test in suspension and agar
diffusion method indicated that the gentamicin addition strongly influenced the antibacterial
activity of HAP/CS/Gent against Staphylococcus aureus and Escherichia coli.
Biocompatibility assays were performed through MTT and DET tests, indicating low
cytotoxicity against MRC-5 and L929 tested cell lines. The coating ability to induce and
promote osteoconduction was proved by ALP assay suggesting that HAP/CS/Gent coating is
an excellent material for bone tissue engineering.
PB  - Targoviste : Valahia University Press
T2  - 9th International Conference of the Chemical Societies of the South-East European Countries
T1  - Gentamicin-loaded bioceramic composite coating aimed for biomedical use
EP  - S4_P_09
SP  - S4_P_09
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Electrophoretic deposition (EPD) process was successfully used for
obtaining composite antibacterial coating of antibiotic gentamicin (Gent), natural polymer
chitosan (CS), and hydroxyapatite (HAP). EPD was carried out on pure titanium (Ti) plates in
a single step from three-component aqueous suspension. The gentamicin influence was
examined through detailed physico-chemical and biological characterization. The formation
of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface was confirmed by
X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier
transform infrared spectroscopy (FTIR) and X-ray photoelectron analysis (XPS). Composite
HAP/CS and HAP/CS/Gent coatings exhibited homogeneous, porous fracture-free surface,
suggesting strong interfacial interactions between HAP and CS. Biological assays pointed to
high potential of HAP/CS/Gent coating for medical applications. Test in suspension and agar
diffusion method indicated that the gentamicin addition strongly influenced the antibacterial
activity of HAP/CS/Gent against Staphylococcus aureus and Escherichia coli.
Biocompatibility assays were performed through MTT and DET tests, indicating low
cytotoxicity against MRC-5 and L929 tested cell lines. The coating ability to induce and
promote osteoconduction was proved by ALP assay suggesting that HAP/CS/Gent coating is
an excellent material for bone tissue engineering.",
publisher = "Targoviste : Valahia University Press",
journal = "9th International Conference of the Chemical Societies of the South-East European Countries",
title = "Gentamicin-loaded bioceramic composite coating aimed for biomedical use",
pages = "S4_P_09-S4_P_09"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2019). Gentamicin-loaded bioceramic composite coating aimed for biomedical use. in 9th International Conference of the Chemical Societies of the South-East European Countries
Targoviste : Valahia University Press., S4_P_09-S4_P_09.

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Mišković-Stanković V. Gentamicin-loaded bioceramic composite coating aimed for biomedical use. in 9th International Conference of the Chemical Societies of the South-East European Countries. 2019;:S4_P_09-S4_P_09..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Gentamicin-loaded bioceramic composite coating aimed for biomedical use" in 9th International Conference of the Chemical Societies of the South-East European Countries (2019):S4_P_09-S4_P_09.