TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Sprecher, Christoph Martin
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1243
AB  - INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.
PB  - Beograd : Savez hemijskih inženjera Srbije
C3  - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
T1  - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
EP  - 58
IS  - 1s
SP  - 58
VL  - 78
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2024",
abstract = "INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.",
publisher = "Beograd : Savez hemijskih inženjera Srbije",
journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices",
title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach",
pages = "58-58",
number = "1s",
volume = "78"
}

Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
Beograd : Savez hemijskih inženjera Srbije., 78(1s), 58-58.

Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):58-58..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):58-58.

TY  - CONF
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/749
AB  - Uvođenje antibiotika u polimer/mineralnu kompozitnu prevlaku poboljšava antibakterijska svojstva prevlaka. Kompozitna prevlaka (hidroksiapatit - HAP, poli(vinilalkohol), hitozan, gentamicin) dobijena je elektroforetskim taloženjem iz četvorokomponentne vodene suspenzije. Primenom termogravimetrijske i rendgenske difrakcione analize pokazano je prisustvo karbonatno-supstituisanog HAP. Antibakterijska aktivnost kompozitne prevlake sa gentamicinom potvrđena je protiv soja S. aureus i E. coli, čineći ovu prevlaku pogodnom za potencijalnu primenu u biomedicini.
AB  - Antibiotic introduction in polymer/mineral coating can improve the antibacterial properties of coatings. Composite coating (hydroxyapatite, poly(vinyl alcohol), chitosan, gentamicin) was fabricated from a four-component aqueous suspension using electrophoretic deposition. Structural and morphological characterization of the coating was investigated by X-ray diffraction and thermogravimetric analysis proving carbonate-substituted HAP. The presence of gentamicin contributed to the antibacterial activity of composite coating against S. aureus and E. coli strains, representing a suitable material for possible application in biomedicine.
PB  - Belgrade : Serbian Chemical Society
C3  - 59. Savetovanje Srpskog hemijskog društva
T1  - Elektroforetski taložene kompozitne prevlake sa gentamicinom
T1  - Electrophoretically deposited gentamicin-loaded composite coating
EP  - 58
SP  - 58
ER  - 

@conference{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Uvođenje antibiotika u polimer/mineralnu kompozitnu prevlaku poboljšava antibakterijska svojstva prevlaka. Kompozitna prevlaka (hidroksiapatit - HAP, poli(vinilalkohol), hitozan, gentamicin) dobijena je elektroforetskim taloženjem iz četvorokomponentne vodene suspenzije. Primenom termogravimetrijske i rendgenske difrakcione analize pokazano je prisustvo karbonatno-supstituisanog HAP. Antibakterijska aktivnost kompozitne prevlake sa gentamicinom potvrđena je protiv soja S. aureus i E. coli, čineći ovu prevlaku pogodnom za potencijalnu primenu u biomedicini., Antibiotic introduction in polymer/mineral coating can improve the antibacterial properties of coatings. Composite coating (hydroxyapatite, poly(vinyl alcohol), chitosan, gentamicin) was fabricated from a four-component aqueous suspension using electrophoretic deposition. Structural and morphological characterization of the coating was investigated by X-ray diffraction and thermogravimetric analysis proving carbonate-substituted HAP. The presence of gentamicin contributed to the antibacterial activity of composite coating against S. aureus and E. coli strains, representing a suitable material for possible application in biomedicine.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59. Savetovanje Srpskog hemijskog društva",
title = "Elektroforetski taložene kompozitne prevlake sa gentamicinom, Electrophoretically deposited gentamicin-loaded composite coating",
pages = "58-58"
}

Đošić, M., Janković, A., Stevanović, M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2023). Elektroforetski taložene kompozitne prevlake sa gentamicinom. in 59. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 58-58.

Đošić M, Janković A, Stevanović M, Vukašinović-Sekulić M, Mišković-Stanković V. Elektroforetski taložene kompozitne prevlake sa gentamicinom. in 59. Savetovanje Srpskog hemijskog društva. 2023;:58-58..

Đošić, Marija, Janković, Ana, Stevanović, Milena, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektroforetski taložene kompozitne prevlake sa gentamicinom" in 59. Savetovanje Srpskog hemijskog društva (2023):58-58.

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/752
AB  - Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers Conference – Materials Science and Engineering
T1  - Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium
EP  - 15
SP  - 15
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers Conference – Materials Science and Engineering",
title = "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium",
pages = "15-15"
}

Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2022). Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 15-15.

Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering. 2022;:15-15..

Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium" in Twentieth Young Researchers Conference – Materials Science and Engineering (2022):15-15.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nesović, Katarina
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/644
PB  - Mary Ann Liebert, Inc, New Rochelle
C3  - Tissue Engineering Part A
T1  - Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations
EP  - S132
SP  - S132
VL  - 28
UR  - conv_946
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nesović, Katarina and Mišković-Stanković, Vesna",
year = "2022",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Tissue Engineering Part A",
title = "Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations",
pages = "S132-S132",
volume = "28",
url = "conv_946"
}

Stevanović, M., Đošić, M., Janković, A., Nesović, K.,& Mišković-Stanković, V.. (2022). Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations. in Tissue Engineering Part A
Mary Ann Liebert, Inc, New Rochelle., 28, S132-S132.
conv_946

Stevanović M, Đošić M, Janković A, Nesović K, Mišković-Stanković V. Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations. in Tissue Engineering Part A. 2022;28:S132-S132.
conv_946 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Nesović, Katarina, Mišković-Stanković, Vesna, "Graphene-loaded bioactive hydroxyapatite coatings on titanium substrate - fundamental in vitro investigations" in Tissue Engineering Part A, 28 (2022):S132-S132,
conv_946 .

Veljović, Đ., Đošić, M., Zvicer, J., Zebić, M., Janaćković, Đ., Janković, A., Matić, T., Miletić, V., Nešović, K., Obradović, B., Osmokrović, A., Petrović, R., Mišković-Stanković, V., Stevanović, M.,& Stojkovska, J.. (2021). New multifunctional biomaterials for medical application. in 35. Međunarodni festival inovacija, znanja i stvaralaštva TESLA FEST 2021
Novi Sad : Savez pronalazača Vojvodine., 5-5.

Veljović Đ, Đošić M, Zvicer J, Zebić M, Janaćković Đ, Janković A, Matić T, Miletić V, Nešović K, Obradović B, Osmokrović A, Petrović R, Mišković-Stanković V, Stevanović M, Stojkovska J. New multifunctional biomaterials for medical application. in 35. Međunarodni festival inovacija, znanja i stvaralaštva TESLA FEST 2021. 2021;:5-5..

Veljović, Đorđe, Đošić, Marija, Zvicer, Jovana, Zebić, Maja, Janaćković, Đorđe, Janković, Ana, Matić, Tamara, Miletić, Vesna, Nešović, Katarina, Obradović, Bojana, Osmokrović, Andrea, Petrović, Rada, Mišković-Stanković, Vesna, Stevanović, Milena, Stojkovska, Jasmina, "New multifunctional biomaterials for medical application" in 35. Međunarodni festival inovacija, znanja i stvaralaštva TESLA FEST 2021 (2021):5-5.

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/572
AB  - This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.
PB  - Elsevier, Amsterdam
T2  - Journal of Materials Research and Technology-Jmr&T
T1  - The chitosan-based bioactive composite coating on titanium
EP  - 4474
SP  - 4461
VL  - 15
DO  - 10.1016/j.jmrt.2021.10.072
UR  - conv_921
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2021",
abstract = "This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Materials Research and Technology-Jmr&T",
title = "The chitosan-based bioactive composite coating on titanium",
pages = "4474-4461",
volume = "15",
doi = "10.1016/j.jmrt.2021.10.072",
url = "conv_921"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2021). The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology-Jmr&T
Elsevier, Amsterdam., 15, 4461-4474.
https://doi.org/10.1016/j.jmrt.2021.10.072
conv_921

Stevanović M, Đošić M, Janković A, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology-Jmr&T. 2021;15:4461-4474.
doi:10.1016/j.jmrt.2021.10.072
conv_921 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "The chitosan-based bioactive composite coating on titanium" in Journal of Materials Research and Technology-Jmr&T, 15 (2021):4461-4474,
https://doi.org/10.1016/j.jmrt.2021.10.072 .,
conv_921 .

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - vuk, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://www.ise-online.org/ise-conferences/annmeet/folder/70th_Annual_meeting-BoA.pdf
UR  - https://ritnms.itnms.ac.rs/handle/123456789/806
AB  - Bone implant materials are increasingly attracting attention in the biomaterials field. Among them, hydroxyapatite (HAP) has stood out as a prospective biomaterial due to excellent osseointegration ability, owing to its similarity with natural bone. However, due to the lack of adhesive and antibacterial properties, it is usually combined with polymers and antibacterial agents. Natural polymer chitosan (CS) was proven as an effective component of HAP-based composites, improving the adhesion and serving as a drug carrier.1 Inclusion of antibiotics into composite biomaterials has gained a lot of attention, as it is thus possible to achieve the desirable antibacterial activity. Gentamicin (Gent) was shown to be one of the most effective antibiotics in treating bone infections. In this work, bioceramic composite HAP/CS and HAP/CS/Gent coatings were successfully assembled on Ti plates by electrophoretic deposition technique (EPD).2 Deposition was carried out at a constant voltage of 5 V and for 12 min in a single step process from three-component aqueous suspension (Figure 1). Bioactive properties of deposited coatings were investigated in vitro, by immersion in simulated body fluid solution (SBF). After 7-day immersion in SBF, field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) were employed to investigate the coatings’ bioactivity, whereas electrochemical impedance spectroscopy (EIS) and polarization measurements were carried out during 28-day immersion in SBF in order to obtain valuable information about osseopromotive coatings’ nature. Biological testing included monitoring of alkaline phosphatase activity (ALP) that is associated with the coatings’ ability to promote new bone growth. The high increase in ALP activity (50 %) relative to the control pointed to the strong coatings’ aptitude for the osseointegration process and new bone growth. FTIR and FE-SEM analysis gave evidence of carbonate-substituted HAP, whose presence is desirable in bone implant applications. The formation of the new apatite layer was also confirmed by electrochemical measurements pointing to the high biomineralization potential of the coatings. Antibacterial activity kinetics in suspension against Staphylococcus aureus and Escherichia coli was also monitored to examine the potential coatings’ application. As expected, the gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gent coating, and the effect was more pronounced against S. aureus.
All the obtained results potentially indicated a strong osseointegration ability of the HAP/CS/Gent coating classifying it as a promising material for bone tissue engineering.
C3  - 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online
T1  - Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and vuk, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Bone implant materials are increasingly attracting attention in the biomaterials field. Among them, hydroxyapatite (HAP) has stood out as a prospective biomaterial due to excellent osseointegration ability, owing to its similarity with natural bone. However, due to the lack of adhesive and antibacterial properties, it is usually combined with polymers and antibacterial agents. Natural polymer chitosan (CS) was proven as an effective component of HAP-based composites, improving the adhesion and serving as a drug carrier.1 Inclusion of antibiotics into composite biomaterials has gained a lot of attention, as it is thus possible to achieve the desirable antibacterial activity. Gentamicin (Gent) was shown to be one of the most effective antibiotics in treating bone infections. In this work, bioceramic composite HAP/CS and HAP/CS/Gent coatings were successfully assembled on Ti plates by electrophoretic deposition technique (EPD).2 Deposition was carried out at a constant voltage of 5 V and for 12 min in a single step process from three-component aqueous suspension (Figure 1). Bioactive properties of deposited coatings were investigated in vitro, by immersion in simulated body fluid solution (SBF). After 7-day immersion in SBF, field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR) were employed to investigate the coatings’ bioactivity, whereas electrochemical impedance spectroscopy (EIS) and polarization measurements were carried out during 28-day immersion in SBF in order to obtain valuable information about osseopromotive coatings’ nature. Biological testing included monitoring of alkaline phosphatase activity (ALP) that is associated with the coatings’ ability to promote new bone growth. The high increase in ALP activity (50 %) relative to the control pointed to the strong coatings’ aptitude for the osseointegration process and new bone growth. FTIR and FE-SEM analysis gave evidence of carbonate-substituted HAP, whose presence is desirable in bone implant applications. The formation of the new apatite layer was also confirmed by electrochemical measurements pointing to the high biomineralization potential of the coatings. Antibacterial activity kinetics in suspension against Staphylococcus aureus and Escherichia coli was also monitored to examine the potential coatings’ application. As expected, the gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gent coating, and the effect was more pronounced against S. aureus.
All the obtained results potentially indicated a strong osseointegration ability of the HAP/CS/Gent coating classifying it as a promising material for bone tissue engineering.",
journal = "71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online",
title = "Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use"
}

Stevanović, M., Đošić, M., Janković, A., vuk, M., Kojić, V.,& Mišković-Stanković, V.. (2020). Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use. in 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online.

Stevanović M, Đošić M, Janković A, vuk M, Kojić V, Mišković-Stanković V. Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use. in 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online. 2020;..

Stevanović, Milena, Đošić, Marija, Janković, Ana, vuk, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Electrophoretically Deposited Bioceramic   Composite Coatings on Ti Substrate Intended for Medical Use" in 71st Annual Meeting of the International Society of Electrochemistry-Belgrade Online (2020).

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nesović, Katarina
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/554
AB  - The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate
EP  - 15445
IS  - 25
SP  - 15433
VL  - 5
DO  - 10.1021/acsomega.0c01583
UR  - conv_884
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nesović, Katarina and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate",
pages = "15445-15433",
number = "25",
volume = "5",
doi = "10.1021/acsomega.0c01583",
url = "conv_884"
}

Stevanović, M., Đošić, M., Janković, A., Nesović, K., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega
Amer Chemical Soc, Washington., 5(25), 15433-15445.
https://doi.org/10.1021/acsomega.0c01583
conv_884

Stevanović M, Đošić M, Janković A, Nesović K, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega. 2020;5(25):15433-15445.
doi:10.1021/acsomega.0c01583
conv_884 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Nesović, Katarina, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate" in ACS Omega, 5, no. 25 (2020):15433-15445,
https://doi.org/10.1021/acsomega.0c01583 .,
conv_884 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukasinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakac, Milkica
AU  - Yop, Rhee Kyong
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/544
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction >3 logarithmic units) compared toEscherichia coli(bacteriostatic, LT 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
UR  - conv_882
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukasinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakac, Milkica and Yop, Rhee Kyong and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect againstStaphylococcus aureus(bactericidal, viable cells number reduction >3 logarithmic units) compared toEscherichia coli(bacteriostatic, LT 3 logarithmic units).MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974",
url = "conv_882"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukasinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakac, M., Yop, R. K.,& Mišković-Stanković, V.. (2020). Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974
conv_882

Stevanović M, Đošić M, Janković A, Kojić V, Vukasinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakac M, Yop RK, Mišković-Stanković V. Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974
conv_882 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukasinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakac, Milkica, Yop, Rhee Kyong, Mišković-Stanković, Vesna, "Antibacterialgraphene-basedhydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 .,
conv_882 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/777
AB  - Electrophoretic deposition (EPD) process was successfully used for
obtaining composite antibacterial coating of antibiotic gentamicin (Gent), natural polymer
chitosan (CS), and hydroxyapatite (HAP). EPD was carried out on pure titanium (Ti) plates in
a single step from three-component aqueous suspension. The gentamicin influence was
examined through detailed physico-chemical and biological characterization. The formation
of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface was confirmed by
X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier
transform infrared spectroscopy (FTIR) and X-ray photoelectron analysis (XPS). Composite
HAP/CS and HAP/CS/Gent coatings exhibited homogeneous, porous fracture-free surface,
suggesting strong interfacial interactions between HAP and CS. Biological assays pointed to
high potential of HAP/CS/Gent coating for medical applications. Test in suspension and agar
diffusion method indicated that the gentamicin addition strongly influenced the antibacterial
activity of HAP/CS/Gent against Staphylococcus aureus and Escherichia coli.
Biocompatibility assays were performed through MTT and DET tests, indicating low
cytotoxicity against MRC-5 and L929 tested cell lines. The coating ability to induce and
promote osteoconduction was proved by ALP assay suggesting that HAP/CS/Gent coating is
an excellent material for bone tissue engineering.
PB  - Targoviste : Valahia University Press
T2  - 9th International Conference of the Chemical Societies of the South-East European Countries
T1  - Gentamicin-loaded bioceramic composite coating aimed for biomedical use
EP  - S4_P_09
SP  - S4_P_09
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Electrophoretic deposition (EPD) process was successfully used for
obtaining composite antibacterial coating of antibiotic gentamicin (Gent), natural polymer
chitosan (CS), and hydroxyapatite (HAP). EPD was carried out on pure titanium (Ti) plates in
a single step from three-component aqueous suspension. The gentamicin influence was
examined through detailed physico-chemical and biological characterization. The formation
of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface was confirmed by
X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier
transform infrared spectroscopy (FTIR) and X-ray photoelectron analysis (XPS). Composite
HAP/CS and HAP/CS/Gent coatings exhibited homogeneous, porous fracture-free surface,
suggesting strong interfacial interactions between HAP and CS. Biological assays pointed to
high potential of HAP/CS/Gent coating for medical applications. Test in suspension and agar
diffusion method indicated that the gentamicin addition strongly influenced the antibacterial
activity of HAP/CS/Gent against Staphylococcus aureus and Escherichia coli.
Biocompatibility assays were performed through MTT and DET tests, indicating low
cytotoxicity against MRC-5 and L929 tested cell lines. The coating ability to induce and
promote osteoconduction was proved by ALP assay suggesting that HAP/CS/Gent coating is
an excellent material for bone tissue engineering.",
publisher = "Targoviste : Valahia University Press",
journal = "9th International Conference of the Chemical Societies of the South-East European Countries",
title = "Gentamicin-loaded bioceramic composite coating aimed for biomedical use",
pages = "S4_P_09-S4_P_09"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2019). Gentamicin-loaded bioceramic composite coating aimed for biomedical use. in 9th International Conference of the Chemical Societies of the South-East European Countries
Targoviste : Valahia University Press., S4_P_09-S4_P_09.

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Mišković-Stanković V. Gentamicin-loaded bioceramic composite coating aimed for biomedical use. in 9th International Conference of the Chemical Societies of the South-East European Countries. 2019;:S4_P_09-S4_P_09..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Gentamicin-loaded bioceramic composite coating aimed for biomedical use" in 9th International Conference of the Chemical Societies of the South-East European Countries (2019):S4_P_09-S4_P_09.

TY  - CONF
AU  - Janković, Ana
AU  - Nešović, Katarina
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/775
AB  - Elektrohemijske metode su korišćene za sintezu različitih kompozita namenjenih za medicinsku primenu kao antibakterijske prevlake za koštane titanske implantate ili kao visoko efikasni antibakterijski hidrogelovi za ubrzano zarastanje rana. Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene suspenzije na titanu. Sadržaj gentamicina je kvantifikovan pomoću tečne hromatografije sa ultravioletnom (UV) detekcijom. Prevlaka HAP/CS/Gent je pokazala dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene. Biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs) dobijen je in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema S.aureus i E.coli je potvrđena testom u suspenziji i agar-difuzionom metodom.
AB  - Electrochemical methods were used for the synthesis of various composites intended for medical use as antibacterial coatings for bone titanium implants or as highly effective antibacterial hydrogels for accelerated wound healing. Electrophoretic deposition (EPD) in a single step at the constant voltage was successfully used to obtain composite coating based on hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium. Gentamicin content was quantified by liquid chromatography with ultraviolet (UV) detection. HAP/CS/Gent coating exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications. Biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs) was produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against S.aureus and E.coli was confirmed by in suspension testing and by disc-diffusion method.
PB  - Belgrade : Serbian Chemical Society
C3  - 56. savetovanje Srpskog hemijskog društva
T1  - Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima
EP  - 28
SP  - 28
ER  - 

@conference{
author = "Janković, Ana and Nešović, Katarina and Stevanović, Milena and Đošić, Marija and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Elektrohemijske metode su korišćene za sintezu različitih kompozita namenjenih za medicinsku primenu kao antibakterijske prevlake za koštane titanske implantate ili kao visoko efikasni antibakterijski hidrogelovi za ubrzano zarastanje rana. Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene suspenzije na titanu. Sadržaj gentamicina je kvantifikovan pomoću tečne hromatografije sa ultravioletnom (UV) detekcijom. Prevlaka HAP/CS/Gent je pokazala dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene. Biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs) dobijen je in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema S.aureus i E.coli je potvrđena testom u suspenziji i agar-difuzionom metodom., Electrochemical methods were used for the synthesis of various composites intended for medical use as antibacterial coatings for bone titanium implants or as highly effective antibacterial hydrogels for accelerated wound healing. Electrophoretic deposition (EPD) in a single step at the constant voltage was successfully used to obtain composite coating based on hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium. Gentamicin content was quantified by liquid chromatography with ultraviolet (UV) detection. HAP/CS/Gent coating exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications. Biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs) was produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against S.aureus and E.coli was confirmed by in suspension testing and by disc-diffusion method.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "56. savetovanje Srpskog hemijskog društva",
title = "Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima",
pages = "28-28"
}

Janković, A., Nešović, K., Stevanović, M., Đošić, M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2019). Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima. in 56. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 28-28.

Janković A, Nešović K, Stevanović M, Đošić M, Vukašinović-Sekulić M, Mišković-Stanković V. Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima. in 56. savetovanje Srpskog hemijskog društva. 2019;:28-28..

Janković, Ana, Nešović, Katarina, Stevanović, Milena, Đošić, Marija, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima" in 56. savetovanje Srpskog hemijskog društva (2019):28-28.

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/773
AB  - Bone diseases and especially prevalent surgical procedures of total joint replacements in
aging population, cause the need for swift development of new biomaterials for bone tissue
implants. Nowadays, Ti implants are by far most commonly used in orthopedics, due to their
excellent mechanical properties and biocompatibility. Having in mind that biomaterials
should stimulate bone tissue regeneration while providing strong support, the Ti implant
surface requires modifications to facilitate improved osteointegration. In this work,
modification of the titanium surface was carried out by depositing novel composite
hydroxyapatite/chitosan/graphene (HAP/CS/Gr) and
hydroxyapatite/chitosan/graphene/gentamicin (HAP/CS/Gr/Gent) coatings. Deposition was
performed by single-step electrophoretic deposition (EPD) technique from aqueous
suspension at constant voltage on pure titanium plates. X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR) and field-emission scanning electron microscopy
(FE-SEM) were used to characterize obtained composites and to explore the graphene
influence on the composites properties. The results of all performed analyses confirmed the
formation of new composite HAP/CS/Gr and HAP/CS/Gr /Gent coatings on Ti. Antibacterial
activity kinetics against Staphylococcus aureus and Escherichia coli was performed in order
to evaluate the antibacterial properties of obtained composites. HAP/CS/Gr/Gent coating
exhibited excellent antibacterial properties, justifying the gentamicin addition to composites.
Encouraging results for potential applications were obtained for cytotoxicity testing towards
MRC-5 and L929 cell lines. Good biocompatibility of composites was proven towards both
cell lines, with slightly more pronounced effect for human MRC-5 cell line. Summarizing the
obtained results it could be safe to state that HAP/CS/Gr/Gent composite represents a
prospective material for bone implant coating.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Eighteenth Young Researchers Conference – Materials Science and Engineering
T1  - Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating
EP  - 18
SP  - 18
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Bone diseases and especially prevalent surgical procedures of total joint replacements in
aging population, cause the need for swift development of new biomaterials for bone tissue
implants. Nowadays, Ti implants are by far most commonly used in orthopedics, due to their
excellent mechanical properties and biocompatibility. Having in mind that biomaterials
should stimulate bone tissue regeneration while providing strong support, the Ti implant
surface requires modifications to facilitate improved osteointegration. In this work,
modification of the titanium surface was carried out by depositing novel composite
hydroxyapatite/chitosan/graphene (HAP/CS/Gr) and
hydroxyapatite/chitosan/graphene/gentamicin (HAP/CS/Gr/Gent) coatings. Deposition was
performed by single-step electrophoretic deposition (EPD) technique from aqueous
suspension at constant voltage on pure titanium plates. X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR) and field-emission scanning electron microscopy
(FE-SEM) were used to characterize obtained composites and to explore the graphene
influence on the composites properties. The results of all performed analyses confirmed the
formation of new composite HAP/CS/Gr and HAP/CS/Gr /Gent coatings on Ti. Antibacterial
activity kinetics against Staphylococcus aureus and Escherichia coli was performed in order
to evaluate the antibacterial properties of obtained composites. HAP/CS/Gr/Gent coating
exhibited excellent antibacterial properties, justifying the gentamicin addition to composites.
Encouraging results for potential applications were obtained for cytotoxicity testing towards
MRC-5 and L929 cell lines. Good biocompatibility of composites was proven towards both
cell lines, with slightly more pronounced effect for human MRC-5 cell line. Summarizing the
obtained results it could be safe to state that HAP/CS/Gr/Gent composite represents a
prospective material for bone implant coating.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Eighteenth Young Researchers Conference – Materials Science and Engineering",
title = "Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating",
pages = "18-18"
}

Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2019). Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating. in Eighteenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 18-18.

Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating. in Eighteenth Young Researchers Conference – Materials Science and Engineering. 2019;:18-18..

Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating" in Eighteenth Young Researchers Conference – Materials Science and Engineering (2019):18-18.

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/502
AB  - Increasing need for improved, compatible bone tissue implants led to the intensive research of novel biomaterials, especially hydroxyapatite (HAP)-based composite materials on titanium and titanium alloy surfaces. Owing to its excellent biocompatibility and osteoinductivity properties, hydroxyapatite is often used as part of composite biomaterials aimed for orthopedic implant applications. In order to overcome persistent problems of bacterial infection, various antimicrobial agents and materials and their incorporation in such medical devices were investigated. This paper represents a comprehensive review of single-step electrodeposition on titanium of hydroxyapatite/chitosan/graphene composite coatings loaded with silver and antibiotic gentamicin as antibacterial agents. The improvement of mechanical and adhesive properties of deposited composite coatings was achieved by graphene and chitosan addition, while desirable antibacterial properties were introduced by including antibiotic gentamicin and silver. The biocompatibility of electrodeposited HAP and HAP-based composite coatings was evaluated by MTT testing, indicating a non-cytotoxic effect and high potential for future medical use as orthopedic implant coating.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents
EP  - 1304
IS  - 11
SP  - 1287
VL  - 84
DO  - 10.2298/JSC190821092S
UR  - conv_868
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Increasing need for improved, compatible bone tissue implants led to the intensive research of novel biomaterials, especially hydroxyapatite (HAP)-based composite materials on titanium and titanium alloy surfaces. Owing to its excellent biocompatibility and osteoinductivity properties, hydroxyapatite is often used as part of composite biomaterials aimed for orthopedic implant applications. In order to overcome persistent problems of bacterial infection, various antimicrobial agents and materials and their incorporation in such medical devices were investigated. This paper represents a comprehensive review of single-step electrodeposition on titanium of hydroxyapatite/chitosan/graphene composite coatings loaded with silver and antibiotic gentamicin as antibacterial agents. The improvement of mechanical and adhesive properties of deposited composite coatings was achieved by graphene and chitosan addition, while desirable antibacterial properties were introduced by including antibiotic gentamicin and silver. The biocompatibility of electrodeposited HAP and HAP-based composite coatings was evaluated by MTT testing, indicating a non-cytotoxic effect and high potential for future medical use as orthopedic implant coating.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents",
pages = "1304-1287",
number = "11",
volume = "84",
doi = "10.2298/JSC190821092S",
url = "conv_868"
}

Stevanović, M., Đošić, M., Janković, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(11), 1287-1304.
https://doi.org/10.2298/JSC190821092S
conv_868

Stevanović M, Đošić M, Janković A, Rhee KY, Mišković-Stanković V. Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents. in Journal of the Serbian Chemical Society. 2019;84(11):1287-1304.
doi:10.2298/JSC190821092S
conv_868 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents" in Journal of the Serbian Chemical Society, 84, no. 11 (2019):1287-1304,
https://doi.org/10.2298/JSC190821092S .,
conv_868 .

Mišković-Stanković, V., Đošić, M., Janković, A.,& Stevanović, M.. (2018). Biokompatibilne kompozitne prevlake hidroksiapatit/hitozan sa gentamicinom na titanu taložene iz vodene suspenzije. in 35. Medunarodna izložba pronalazaka, novih tehnologija, industrijskog dizajna i IV Kup nacija mladih inovatora
Beograd : Savez pronalazača Beograd., 77-78.

Mišković-Stanković V, Đošić M, Janković A, Stevanović M. Biokompatibilne kompozitne prevlake hidroksiapatit/hitozan sa gentamicinom na titanu taložene iz vodene suspenzije. in 35. Medunarodna izložba pronalazaka, novih tehnologija, industrijskog dizajna i IV Kup nacija mladih inovatora. 2018;:77-78..

Mišković-Stanković, Vesna, Đošić, Marija, Janković, Ana, Stevanović, Milena, "Biokompatibilne kompozitne prevlake hidroksiapatit/hitozan sa gentamicinom na titanu taložene iz vodene suspenzije" in 35. Medunarodna izložba pronalazaka, novih tehnologija, industrijskog dizajna i IV Kup nacija mladih inovatora (2018):77-78.

TY  - CONF
AU  - Janković, Ana
AU  - Nešović, Katarina
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/769
AB  - Elektrohemijske metode su koris ćene za sintezu razlic itih kompozita namenjenih za aplikacije u medicini kao antibakterijske prevlake na koštanim titanskim implantatima ili kao visoko efikasni antimikrobni hidrogelovi za ubrzano zarastanje rana.
Prvi sintetisani materijal je kompozitna prevlaka hidroksiapatita (HAP) sa prirodnim polimerom hitozanom (CS) koja uključuje antibiotik gentamicin, dobijena metodom elektroforetskog taloz enja (EPD). EPD se izvodi pri konstantnom naponu na ploc icama c istog titana iz vodene suspenzije. Na ovaj nac in dobijena porozna homogena prevlaka ima poboljs anu mehanic ku c vrstoću i povoljne antibakterijske osobine. Prevlaka HAP/CS/Gent okarakterisana je difrakcijom X-zraka (XRD), infracrvenom spektroskopijom sa Furijeovom transformacijom (FT-IR) i skenirajućom elektronskom mikroskopijom (FE-SEM). Dobijeni rezultati potvrdili su formiranje nove kompozitne prevlake HAP/CS/Gent. Citotoksičnost prema dvema vrstama ćelijskih linija ispitana je koris ćenjem testa iskljuc ivanja tripan plave boje (DET) i MTT testa za procenu ćelijske metaboličke aktivnosti. Prevlaka sa antibiotikom (HAP/CS/Gent) pokazala je dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, dok je istovremeno zadržala nisku citotoksičnost, što ukazuje na visok potencijal za biomedicinske primene.
Drugi sintetisani materijal je biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs). AgNPs su dobijene in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Korišćene su dve koncentracije CHI (0,1 mas.% and 0,5 mas.%), u cilju ispitivanja uticaja sadržaja CHI na svojstva nankompozitnih hidrogelova. Nanokompoziti Ag/PVA/CHI/Gr su dalje karakterisani metodom skenirajuće elektronske mikroskopije (SEM), Raman spektroskopije i rendgenske fotoelektronske spektroskopije (XPS). Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema Staphylococcus aureus i Escherichia coli je potvrđena agar-difuzionim testom, dok je citotoksičnost ispitana direktnim testom isključenja sa bojom tripan-plavo (DET).
AB  - Electrochemical methods were employed for synthesis of various composites intended for applications in medicine as antibacterial coatings on titanium bone implants or as highly efficient antimicrobial hydrogels for accelerated wound healing.
The first synthesized material is a composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS) which includes an antibiotic gentamicin, obtained by the method of electrophoretic deposition (EPD). EPD was carried out at the constant voltage on pure titanium plates from an aqueous suspension. Thus assembled porous homogenous coating would gain improved mechanical strength and favorable antibacterial properties. HAP/CS/Gent coating was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). Obtained results confirmed the formation of a new composite coating HAP/CS/Gent. Cytotoxicity against two types of cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The antibiotic loaded coating (HAP/CS/Gent) exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating the high potential for biomedical applications.
The second synthesized material is biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs). AgNPs were produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Two concentrations of CHI (0.1 wt% and 0.5 wt%) were used in order to investigate the influence of CHI content on the properties of nanocomposite hydrogels. The Ag/PVA/CHI/Gr nanocomposites were further characterized by scanning electron microscopy (SEM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against Staphylococcus aureus and Escherichia coli was confirmed by disc-diffusion tests, while low cytotoxicity was confirmed using trypan blue dye-exclusion test (DET).
PB  - Beograd : UDRUŽENJE MIKROBIOLOGA SRBIJE
C3  - XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO
T1  - Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene
EP  - 163
SP  - 162
ER  - 

@conference{
author = "Janković, Ana and Nešović, Katarina and Stevanović, Milena and Đošić, Marija and Kojić, Vesna and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Elektrohemijske metode su koris ćene za sintezu razlic itih kompozita namenjenih za aplikacije u medicini kao antibakterijske prevlake na koštanim titanskim implantatima ili kao visoko efikasni antimikrobni hidrogelovi za ubrzano zarastanje rana.
Prvi sintetisani materijal je kompozitna prevlaka hidroksiapatita (HAP) sa prirodnim polimerom hitozanom (CS) koja uključuje antibiotik gentamicin, dobijena metodom elektroforetskog taloz enja (EPD). EPD se izvodi pri konstantnom naponu na ploc icama c istog titana iz vodene suspenzije. Na ovaj nac in dobijena porozna homogena prevlaka ima poboljs anu mehanic ku c vrstoću i povoljne antibakterijske osobine. Prevlaka HAP/CS/Gent okarakterisana je difrakcijom X-zraka (XRD), infracrvenom spektroskopijom sa Furijeovom transformacijom (FT-IR) i skenirajućom elektronskom mikroskopijom (FE-SEM). Dobijeni rezultati potvrdili su formiranje nove kompozitne prevlake HAP/CS/Gent. Citotoksičnost prema dvema vrstama ćelijskih linija ispitana je koris ćenjem testa iskljuc ivanja tripan plave boje (DET) i MTT testa za procenu ćelijske metaboličke aktivnosti. Prevlaka sa antibiotikom (HAP/CS/Gent) pokazala je dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, dok je istovremeno zadržala nisku citotoksičnost, što ukazuje na visok potencijal za biomedicinske primene.
Drugi sintetisani materijal je biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs). AgNPs su dobijene in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Korišćene su dve koncentracije CHI (0,1 mas.% and 0,5 mas.%), u cilju ispitivanja uticaja sadržaja CHI na svojstva nankompozitnih hidrogelova. Nanokompoziti Ag/PVA/CHI/Gr su dalje karakterisani metodom skenirajuće elektronske mikroskopije (SEM), Raman spektroskopije i rendgenske fotoelektronske spektroskopije (XPS). Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema Staphylococcus aureus i Escherichia coli je potvrđena agar-difuzionim testom, dok je citotoksičnost ispitana direktnim testom isključenja sa bojom tripan-plavo (DET)., Electrochemical methods were employed for synthesis of various composites intended for applications in medicine as antibacterial coatings on titanium bone implants or as highly efficient antimicrobial hydrogels for accelerated wound healing.
The first synthesized material is a composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS) which includes an antibiotic gentamicin, obtained by the method of electrophoretic deposition (EPD). EPD was carried out at the constant voltage on pure titanium plates from an aqueous suspension. Thus assembled porous homogenous coating would gain improved mechanical strength and favorable antibacterial properties. HAP/CS/Gent coating was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). Obtained results confirmed the formation of a new composite coating HAP/CS/Gent. Cytotoxicity against two types of cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The antibiotic loaded coating (HAP/CS/Gent) exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating the high potential for biomedical applications.
The second synthesized material is biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs). AgNPs were produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Two concentrations of CHI (0.1 wt% and 0.5 wt%) were used in order to investigate the influence of CHI content on the properties of nanocomposite hydrogels. The Ag/PVA/CHI/Gr nanocomposites were further characterized by scanning electron microscopy (SEM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against Staphylococcus aureus and Escherichia coli was confirmed by disc-diffusion tests, while low cytotoxicity was confirmed using trypan blue dye-exclusion test (DET).",
publisher = "Beograd : UDRUŽENJE MIKROBIOLOGA SRBIJE",
journal = "XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO",
title = "Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene",
pages = "163-162"
}

Janković, A., Nešović, K., Stevanović, M., Đošić, M., Kojić, V., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2018). Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO
Beograd : UDRUŽENJE MIKROBIOLOGA SRBIJE., 162-163.

Janković A, Nešović K, Stevanović M, Đošić M, Kojić V, Vukašinović-Sekulić M, Mišković-Stanković V. Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO. 2018;:162-163..

Janković, Ana, Nešović, Katarina, Stevanović, Milena, Đošić, Marija, Kojić, Vesna, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene" in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO (2018):162-163.

TY  - CONF
AU  - Đošić, Marija
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/765
AB  - Elektroforetsko taloženje je efikasna elektrohemijska metoda za dobijanje višekomponentnih
biokeramičkih prevlaka na metalnom supstratu. Za formiranje biomaterijala za
koštane implantate, najbolji rezultati su dobijeni kod prevlaka koje predstavljaju kombinaciju
mineralne faze i polimera. U cilju dobijanja bioaktivnih prevlaka na titanu primenom
elektroforetskog taloženja, urađena je optimizacija uslova taloženja iz trokomponentne
vodene suspenzije hidroksiapatita (HAP), hitozana (CS) i antibiotika gentamicina (Gent), pri
različitim konstantnim vrednostima napona i vremena taloženja. Karakterizacija prevlaka je
izvršena primenom rendgenske difrakcione analize (XRD) i rendgenske fotoelektronske
spektroskopije (XPS). Citotoksičnost dobijenih prevlaka je ispitana MTT testom, dok je
antibakterijska aktivnost prevlake sa gentamicinom potvrđena primenom testa u suspenziji.
Na osnovu dobijenih rezultata, potvrđeno je da trokomponentna biokompatibilna prevlaka
HAP/CS/Gent ima veliki potencijal za upotrebu u biomedicini.
AB  - Electrophoretic deposition is a suitable method for obtaining multicomponent bioceramic
coatings on titanium (Ti). For hard tissue implants, the best results are achieved for the
coating composed of mineral phase and a polymer. First step in producing bioactive
coatings on Ti using electrophoretic deposition process was the optimization of deposition
parameters for three-component aqueous suspension, containing hydroxyapatite (HAP),
chitosan (CS) and gentamicin (Gent), at constant values of applied voltages and different
deposition times. The coatings obtained were characterized by X-ray diffraction (XRD) and
X-ray photoelectron spectroscopy (XPS). Cytotoxicity of the coatings was evaluated by MTT
assay, and the antibacterial activity of gentamicin loaded coating was verified using test in
suspension. Based on the experimental results, the formation of a new HAP/CS/Gent
composite coating with future prospect for biomedical use was confirmed.
PB  - Belgrade : Serbian Chemical Society
C3  - 55. savetovanje Srpskog hemijskog društva
T1  - Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu
EP  - 22
SP  - 22
ER  - 

@conference{
author = "Đošić, Marija and Stevanović, Milena and Janković, Ana and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Elektroforetsko taloženje je efikasna elektrohemijska metoda za dobijanje višekomponentnih
biokeramičkih prevlaka na metalnom supstratu. Za formiranje biomaterijala za
koštane implantate, najbolji rezultati su dobijeni kod prevlaka koje predstavljaju kombinaciju
mineralne faze i polimera. U cilju dobijanja bioaktivnih prevlaka na titanu primenom
elektroforetskog taloženja, urađena je optimizacija uslova taloženja iz trokomponentne
vodene suspenzije hidroksiapatita (HAP), hitozana (CS) i antibiotika gentamicina (Gent), pri
različitim konstantnim vrednostima napona i vremena taloženja. Karakterizacija prevlaka je
izvršena primenom rendgenske difrakcione analize (XRD) i rendgenske fotoelektronske
spektroskopije (XPS). Citotoksičnost dobijenih prevlaka je ispitana MTT testom, dok je
antibakterijska aktivnost prevlake sa gentamicinom potvrđena primenom testa u suspenziji.
Na osnovu dobijenih rezultata, potvrđeno je da trokomponentna biokompatibilna prevlaka
HAP/CS/Gent ima veliki potencijal za upotrebu u biomedicini., Electrophoretic deposition is a suitable method for obtaining multicomponent bioceramic
coatings on titanium (Ti). For hard tissue implants, the best results are achieved for the
coating composed of mineral phase and a polymer. First step in producing bioactive
coatings on Ti using electrophoretic deposition process was the optimization of deposition
parameters for three-component aqueous suspension, containing hydroxyapatite (HAP),
chitosan (CS) and gentamicin (Gent), at constant values of applied voltages and different
deposition times. The coatings obtained were characterized by X-ray diffraction (XRD) and
X-ray photoelectron spectroscopy (XPS). Cytotoxicity of the coatings was evaluated by MTT
assay, and the antibacterial activity of gentamicin loaded coating was verified using test in
suspension. Based on the experimental results, the formation of a new HAP/CS/Gent
composite coating with future prospect for biomedical use was confirmed.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "55. savetovanje Srpskog hemijskog društva",
title = "Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu",
pages = "22-22"
}

Đošić, M., Stevanović, M., Janković, A., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2018). Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu. in 55. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 22-22.

Đošić M, Stevanović M, Janković A, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu. in 55. savetovanje Srpskog hemijskog društva. 2018;:22-22..

Đošić, Marija, Stevanović, Milena, Janković, Ana, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu" in 55. savetovanje Srpskog hemijskog društva (2018):22-22.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/763
AB  - Novel composite coating based on hydroxyapatite, chitosan and gentamicin
(HAP/CS/Gent) was successfully obtained by single-step electrophoretic deposition
process from aqueous suspension. In order to investigate the potential application of
composite HAP/CS/Gent coating, biological properties were examined. The
antibacterial activity of composite coating was tested using an agar diffusion method
and test in suspension against two bacteria strains- Staphylococcus aureus and
Escherichia coli. Biocompatibility was evaluated by cytotoxicity testing, towards MRC-
5 and L929 cell lines using MTT and DET tests. The ability of biomineralization was
tested by ALP assay towards MRC-5 and L929 cell lines as model cells. Excellent
antibacterial properties were observed for HAP/CS/Gent coating, especially in case of
S. aureus. DET and MTT assays indicated low cytotoxicity against MRC-5 and L929
tested cell lines, indicating good biocompatibility. High ALP level in the cell extract
implicates the good bioactivity of HAP/CS/Gent coating. Due to the compatibility of
the cells, MRC-5 cell line responded better in ALP assay.
Due to good antibacterial and anti-biofilm efficacy, good biocompatibility and ability to
promote biomineralization, composite HAP/CS/Gent coating can be can be considered
for future medical application as hard tissue implant.
PB  - Belgrade : Serbian Chemical Society
C3  - Šesta konferencija mladih hemičara Srbije
T1  - Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating
EP  - 102
SP  - 102
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Novel composite coating based on hydroxyapatite, chitosan and gentamicin
(HAP/CS/Gent) was successfully obtained by single-step electrophoretic deposition
process from aqueous suspension. In order to investigate the potential application of
composite HAP/CS/Gent coating, biological properties were examined. The
antibacterial activity of composite coating was tested using an agar diffusion method
and test in suspension against two bacteria strains- Staphylococcus aureus and
Escherichia coli. Biocompatibility was evaluated by cytotoxicity testing, towards MRC-
5 and L929 cell lines using MTT and DET tests. The ability of biomineralization was
tested by ALP assay towards MRC-5 and L929 cell lines as model cells. Excellent
antibacterial properties were observed for HAP/CS/Gent coating, especially in case of
S. aureus. DET and MTT assays indicated low cytotoxicity against MRC-5 and L929
tested cell lines, indicating good biocompatibility. High ALP level in the cell extract
implicates the good bioactivity of HAP/CS/Gent coating. Due to the compatibility of
the cells, MRC-5 cell line responded better in ALP assay.
Due to good antibacterial and anti-biofilm efficacy, good biocompatibility and ability to
promote biomineralization, composite HAP/CS/Gent coating can be can be considered
for future medical application as hard tissue implant.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Šesta konferencija mladih hemičara Srbije",
title = "Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating",
pages = "102-102"
}

Stevanović, M., Đošić, M., Janković, A.,& Mišković-Stanković, V.. (2018). Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating. in Šesta konferencija mladih hemičara Srbije
Belgrade : Serbian Chemical Society., 102-102.

Stevanović M, Đošić M, Janković A, Mišković-Stanković V. Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating. in Šesta konferencija mladih hemičara Srbije. 2018;:102-102..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating" in Šesta konferencija mladih hemičara Srbije (2018):102-102.

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukasinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakac, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/468
AB  - Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
PB  - Amer Chemical Soc, Washington
T2  - ACS Biomaterials Science & Engineering
T1  - Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
EP  - 4007
IS  - 12
SP  - 3994
VL  - 4
DO  - 10.1021/acsbiomaterials.8b00859
UR  - conv_839
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukasinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakac, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Biomaterials Science & Engineering",
title = "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium",
pages = "4007-3994",
number = "12",
volume = "4",
doi = "10.1021/acsbiomaterials.8b00859",
url = "conv_839"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukasinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakac, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering
Amer Chemical Soc, Washington., 4(12), 3994-4007.
https://doi.org/10.1021/acsbiomaterials.8b00859
conv_839

Stevanović M, Đošić M, Janković A, Kojić V, Vukasinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakac M, Rhee KY, Mišković-Stanković V. Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering. 2018;4(12):3994-4007.
doi:10.1021/acsbiomaterials.8b00859
conv_839 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukasinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakac, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium" in ACS Biomaterials Science & Engineering, 4, no. 12 (2018):3994-4007,
https://doi.org/10.1021/acsbiomaterials.8b00859 .,
conv_839 .

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2017
UR  - https://ritnms.itnms.ac.rs/handle/123456789/760
AB  - A broad spectrum antibiotic-gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymer chitosan (CS) using electrophoretic deposition (EPD) technique.
EPD was carried out at the constant voltage on pure titanium plates from an aqueous
suspension. The aim was to assemble porous homogenous coating with improved mechanical
strength and favourable antibacterial properties. HAP/CS/Gent coating was characterized by
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR),
thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM).
Obtained results confirmed the formation of a new composite coating HAP/CS/Gent.
In the XRD spectrum of HAP/CS/Gent only characteristic crystal planes of HAP were
detected. FTIR analysis confirmed the formation of intermolecular and intramolecular
hydrogen bonds in the composite coating. TGA analysis was employed to investigate the
thermal stability of HAP/CS/Gent. The bioactivity of HAP/CS/Gent composite coating was
proved by SEM analysis. The obtained SEM micrographs indicate the biomimetic growth of
a new apatite layer after immersion in SBF. The antibiotic loaded coating (HAP/CS/Gent)
exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli,
indicating the high potential for biomedical applications. Additionally, the results have
shown that the antibacterial effect of HAP/CS/Gent was more pronounced against S. aureus
compared to E. coli.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Sixteenth Young Researchers Conference – Materials Science and Engineering
T1  - Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin
EP  - 14
SP  - 14
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2017",
abstract = "A broad spectrum antibiotic-gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymer chitosan (CS) using electrophoretic deposition (EPD) technique.
EPD was carried out at the constant voltage on pure titanium plates from an aqueous
suspension. The aim was to assemble porous homogenous coating with improved mechanical
strength and favourable antibacterial properties. HAP/CS/Gent coating was characterized by
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR),
thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM).
Obtained results confirmed the formation of a new composite coating HAP/CS/Gent.
In the XRD spectrum of HAP/CS/Gent only characteristic crystal planes of HAP were
detected. FTIR analysis confirmed the formation of intermolecular and intramolecular
hydrogen bonds in the composite coating. TGA analysis was employed to investigate the
thermal stability of HAP/CS/Gent. The bioactivity of HAP/CS/Gent composite coating was
proved by SEM analysis. The obtained SEM micrographs indicate the biomimetic growth of
a new apatite layer after immersion in SBF. The antibiotic loaded coating (HAP/CS/Gent)
exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli,
indicating the high potential for biomedical applications. Additionally, the results have
shown that the antibacterial effect of HAP/CS/Gent was more pronounced against S. aureus
compared to E. coli.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Sixteenth Young Researchers Conference – Materials Science and Engineering",
title = "Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin",
pages = "14-14"
}

Stevanović, M., Đošić, M., Janković, A., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2017). Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin. in Sixteenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 14-14.

Stevanović M, Đošić M, Janković A, Vukašinović-Sekulić M, Mišković-Stanković V. Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin. in Sixteenth Young Researchers Conference – Materials Science and Engineering. 2017;:14-14..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin" in Sixteenth Young Researchers Conference – Materials Science and Engineering (2017):14-14.