TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/572
AB  - This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.
PB  - Elsevier, Amsterdam
T2  - Journal of Materials Research and Technology-Jmr&T
T1  - The chitosan-based bioactive composite coating on titanium
EP  - 4474
SP  - 4461
VL  - 15
DO  - 10.1016/j.jmrt.2021.10.072
UR  - conv_921
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2021",
abstract = "This paper describes a promising electrophoretic deposition (EPD) approach for developing composite coatings based on chitosan with the additional components of hydroxyapatite, graphene, and gentamicin on titanium substrate. Bioactive properties were investigated in vitro by immersing the coatings in simulated body fluid (SBF) at 37 degrees C. The newly formed biomimetic layer on the top of the deposited chitosan-based coatings on Ti was confirmed by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy and electrochemical measurements, while coatings' bioactivity was proved by alkaline phosphatase activity assay in MRC-5 and L929 tested cell lines. The biocompatibility towards MRC-5 and L929 cell lines was investigated by dye exclusion test (DET) implying the non-cytotoxic effect of coatings. Gentamicin release studies were monitored during 21-day by high-performance liquid chromatography coupled with mass spectrometry, indicating rapid release of gentamicin (approximate to 40%) in the first 48 h and more than 60% after 14 days.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Materials Research and Technology-Jmr&T",
title = "The chitosan-based bioactive composite coating on titanium",
pages = "4474-4461",
volume = "15",
doi = "10.1016/j.jmrt.2021.10.072",
url = "conv_921"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2021). The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology-Jmr&T
Elsevier, Amsterdam., 15, 4461-4474.
https://doi.org/10.1016/j.jmrt.2021.10.072
conv_921

Stevanović M, Đošić M, Janković A, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. The chitosan-based bioactive composite coating on titanium. in Journal of Materials Research and Technology-Jmr&T. 2021;15:4461-4474.
doi:10.1016/j.jmrt.2021.10.072
conv_921 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "The chitosan-based bioactive composite coating on titanium" in Journal of Materials Research and Technology-Jmr&T, 15 (2021):4461-4474,
https://doi.org/10.1016/j.jmrt.2021.10.072 .,
conv_921 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/755
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley
T2  - Journal of Biomedical Materials Research
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley",
journal = "Journal of Biomedical Materials Research",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research
Wiley., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Rhee KY, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Nesović, Katarina
AU  - Kojić, Vesna
AU  - Stojanović, Jovica
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/554
AB  - The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate
EP  - 15445
IS  - 25
SP  - 15433
VL  - 5
DO  - 10.1021/acsomega.0c01583
UR  - conv_884
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Nesović, Katarina and Kojić, Vesna and Stojanović, Jovica and Grujić, Svetlana and Matić-Bujagić, Ivana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "The electrophoretic deposition process (EPD) was utilized to produce bioactive hydroxyapatite/chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) coatings on titanium. The bioactivity of newly synthesized composite coatings was investigated in the simulated body fluid (SBF) and examined by X-ray diffraction, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy. The obtained results revealed carbonate-substituted hydroxyapatite after immersion in SBF, emphasizing the similarity of the biomimetically grown HAP with the naturally occurring apatite in the bone. The formation of biomimetic HAP was confirmed by electrochemical impedance spectroscopy and polarization measurements, through the decrease in corrosion current density and coating capacitance values after 28-day immersion in SBF. The osseointegration ability was further validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings (significant increase in ALP levels for both HAP/CS (3.206 U mL(-1)) and HAP/CS/Gent (4.039 U mL(-1)) coatings, compared to the control (0.900 U mL(-1))). Drug-release kinetics was investigated in deionized water at 37 degrees C by high-performance liquid chromatography coupled with mass spectrometry. Release profiles revealed the beneficial "burst-release effect" (similar to 21% of gentamicin released in the first 48 h) as a potentially promising solution against the biofilm formation in the initial period. When tested against human and mice fibroblast cells (MRC-5 and L929), both composite coatings showed a noncytotoxic effect (viability >85%), providing a promising basis for further medical application trials.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate",
pages = "15445-15433",
number = "25",
volume = "5",
doi = "10.1021/acsomega.0c01583",
url = "conv_884"
}

Stevanović, M., Đošić, M., Janković, A., Nesović, K., Kojić, V., Stojanović, J., Grujić, S., Matić-Bujagić, I., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega
Amer Chemical Soc, Washington., 5(25), 15433-15445.
https://doi.org/10.1021/acsomega.0c01583
conv_884

Stevanović M, Đošić M, Janković A, Nesović K, Kojić V, Stojanović J, Grujić S, Matić-Bujagić I, Rhee KY, Mišković-Stanković V. Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate. in ACS Omega. 2020;5(25):15433-15445.
doi:10.1021/acsomega.0c01583
conv_884 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Nesović, Katarina, Kojić, Vesna, Stojanović, Jovica, Grujić, Svetlana, Matić-Bujagić, Ivana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Assessing the Bioactivity of Gentamicin-Preloaded Hydroxyapatite/Chitosan Composite Coating on Titanium Substrate" in ACS Omega, 5, no. 25 (2020):15433-15445,
https://doi.org/10.1021/acsomega.0c01583 .,
conv_884 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/502
AB  - Increasing need for improved, compatible bone tissue implants led to the intensive research of novel biomaterials, especially hydroxyapatite (HAP)-based composite materials on titanium and titanium alloy surfaces. Owing to its excellent biocompatibility and osteoinductivity properties, hydroxyapatite is often used as part of composite biomaterials aimed for orthopedic implant applications. In order to overcome persistent problems of bacterial infection, various antimicrobial agents and materials and their incorporation in such medical devices were investigated. This paper represents a comprehensive review of single-step electrodeposition on titanium of hydroxyapatite/chitosan/graphene composite coatings loaded with silver and antibiotic gentamicin as antibacterial agents. The improvement of mechanical and adhesive properties of deposited composite coatings was achieved by graphene and chitosan addition, while desirable antibacterial properties were introduced by including antibiotic gentamicin and silver. The biocompatibility of electrodeposited HAP and HAP-based composite coatings was evaluated by MTT testing, indicating a non-cytotoxic effect and high potential for future medical use as orthopedic implant coating.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents
EP  - 1304
IS  - 11
SP  - 1287
VL  - 84
DO  - 10.2298/JSC190821092S
UR  - conv_868
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Increasing need for improved, compatible bone tissue implants led to the intensive research of novel biomaterials, especially hydroxyapatite (HAP)-based composite materials on titanium and titanium alloy surfaces. Owing to its excellent biocompatibility and osteoinductivity properties, hydroxyapatite is often used as part of composite biomaterials aimed for orthopedic implant applications. In order to overcome persistent problems of bacterial infection, various antimicrobial agents and materials and their incorporation in such medical devices were investigated. This paper represents a comprehensive review of single-step electrodeposition on titanium of hydroxyapatite/chitosan/graphene composite coatings loaded with silver and antibiotic gentamicin as antibacterial agents. The improvement of mechanical and adhesive properties of deposited composite coatings was achieved by graphene and chitosan addition, while desirable antibacterial properties were introduced by including antibiotic gentamicin and silver. The biocompatibility of electrodeposited HAP and HAP-based composite coatings was evaluated by MTT testing, indicating a non-cytotoxic effect and high potential for future medical use as orthopedic implant coating.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents",
pages = "1304-1287",
number = "11",
volume = "84",
doi = "10.2298/JSC190821092S",
url = "conv_868"
}

Stevanović, M., Đošić, M., Janković, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2019). Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(11), 1287-1304.
https://doi.org/10.2298/JSC190821092S
conv_868

Stevanović M, Đošić M, Janković A, Rhee KY, Mišković-Stanković V. Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents. in Journal of the Serbian Chemical Society. 2019;84(11):1287-1304.
doi:10.2298/JSC190821092S
conv_868 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Electrophoretically deposited hydroxyapatite-based composite coatings loaded with silver and gentamicin as antibacterial agents" in Journal of the Serbian Chemical Society, 84, no. 11 (2019):1287-1304,
https://doi.org/10.2298/JSC190821092S .,
conv_868 .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/484
AB  - Brzi razvoj istraživanja nanomaterijala koji sadrže grafen otkrio je da se njihova izuzetna svojstva mogu koristiti za biomedicinske primene, posebno u inženjerstvu tkiva i kao nosači lekova. U ovom radu su prikazani rezultati istraživanja biokompozitnih prevlaka na bazi hidroksiapatita koje su dobijene elektroforetskim taloženjem na titanskom supstratu i ojačane nanočestičnim grafenom, kao potencijalnih kandidata za implante koštanog tkiva.
AB  - Fast ongoing research of graphene-based nanomaterials reveals that their exceptional properties could be utized for biomedical applications, especially in tissue engineering and drug delivery. Here, we summarized the results obtained on hydroxyapatite based biocomposite coatings produced by electrophoretic deposition on titanium substrate and reinforced by nanosized graphene, as potential candidates for hard tissue implants.
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu
T1  - Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium
EP  - 306
IS  - 2
SP  - 293
VL  - 59
DO  - 10.5937/ZasMat1802293D
UR  - conv_156
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Brzi razvoj istraživanja nanomaterijala koji sadrže grafen otkrio je da se njihova izuzetna svojstva mogu koristiti za biomedicinske primene, posebno u inženjerstvu tkiva i kao nosači lekova. U ovom radu su prikazani rezultati istraživanja biokompozitnih prevlaka na bazi hidroksiapatita koje su dobijene elektroforetskim taloženjem na titanskom supstratu i ojačane nanočestičnim grafenom, kao potencijalnih kandidata za implante koštanog tkiva., Fast ongoing research of graphene-based nanomaterials reveals that their exceptional properties could be utized for biomedical applications, especially in tissue engineering and drug delivery. Here, we summarized the results obtained on hydroxyapatite based biocomposite coatings produced by electrophoretic deposition on titanium substrate and reinforced by nanosized graphene, as potential candidates for hard tissue implants.",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu, Graphene reinforced hydroxyapatite biocomposite coatings obtained by electrophoretic deposition on titanium",
pages = "306-293",
number = "2",
volume = "59",
doi = "10.5937/ZasMat1802293D",
url = "conv_156"
}

Đošić, M., Janković, A., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu. in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 59(2), 293-306.
https://doi.org/10.5937/ZasMat1802293D
conv_156

Đošić M, Janković A, Rhee KY, Mišković-Stanković V. Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu. in Zaštita materijala. 2018;59(2):293-306.
doi:10.5937/ZasMat1802293D
conv_156 .

Đošić, Marija, Janković, Ana, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Biokompozitne prevlake hidroksiapatita ojačane grafenom, dobijene elektroforetskim taloženjem na titanu" in Zaštita materijala, 59, no. 2 (2018):293-306,
https://doi.org/10.5937/ZasMat1802293D .,
conv_156 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukasinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakac, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/468
AB  - Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
PB  - Amer Chemical Soc, Washington
T2  - ACS Biomaterials Science & Engineering
T1  - Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
EP  - 4007
IS  - 12
SP  - 3994
VL  - 4
DO  - 10.1021/acsbiomaterials.8b00859
UR  - conv_839
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukasinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakac, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Biomaterials Science & Engineering",
title = "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium",
pages = "4007-3994",
number = "12",
volume = "4",
doi = "10.1021/acsbiomaterials.8b00859",
url = "conv_839"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukasinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakac, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering
Amer Chemical Soc, Washington., 4(12), 3994-4007.
https://doi.org/10.1021/acsbiomaterials.8b00859
conv_839

Stevanović M, Đošić M, Janković A, Kojić V, Vukasinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakac M, Rhee KY, Mišković-Stanković V. Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering. 2018;4(12):3994-4007.
doi:10.1021/acsbiomaterials.8b00859
conv_839 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukasinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakac, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium" in ACS Biomaterials Science & Engineering, 4, no. 12 (2018):3994-4007,
https://doi.org/10.1021/acsbiomaterials.8b00859 .,
conv_839 .

TY  - JOUR
AU  - Đošić, Marija
AU  - Eraković, Sanja
AU  - Janković, Ana
AU  - Vukasinović-Sekulić, Maja
AU  - Matić, Ivana Z.
AU  - Stojanović, Jovica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
AU  - Park, Soo-Jin
PY  - 2017
UR  - https://ritnms.itnms.ac.rs/handle/123456789/428
AB  - Graphene (Gr) and natural polymer chitosan (CS) were introduced to hydroxyapatite (HAP) to produce a three-component composite coating, which was fabricated by cathodic electrophoretic deposition on Ti substrates in an ethanol suspension. These HAP/CS/Gr coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements and found that the graphene into HAP/CS composites significantly improves their morphology, thermal stability, and bioactivity. Both HAP/CS and HAP/CS/Gr composite coatings are classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC), while antibacterial activity against Staphylococcus aureus and Escherichia coil could not be verified.
PB  - Elsevier Science Inc, New York
T2  - Journal of Industrial and Engineering Chemistry
T1  - In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene
EP  - 347
SP  - 336
VL  - 47
DO  - 10.1016/j.jiec.2016.12.004
UR  - conv_787
ER  - 

@article{
author = "Đošić, Marija and Eraković, Sanja and Janković, Ana and Vukasinović-Sekulić, Maja and Matić, Ivana Z. and Stojanović, Jovica and Rhee, Kyong Yop and Mišković-Stanković, Vesna and Park, Soo-Jin",
year = "2017",
abstract = "Graphene (Gr) and natural polymer chitosan (CS) were introduced to hydroxyapatite (HAP) to produce a three-component composite coating, which was fabricated by cathodic electrophoretic deposition on Ti substrates in an ethanol suspension. These HAP/CS/Gr coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements and found that the graphene into HAP/CS composites significantly improves their morphology, thermal stability, and bioactivity. Both HAP/CS and HAP/CS/Gr composite coatings are classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC), while antibacterial activity against Staphylococcus aureus and Escherichia coil could not be verified.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Industrial and Engineering Chemistry",
title = "In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene",
pages = "347-336",
volume = "47",
doi = "10.1016/j.jiec.2016.12.004",
url = "conv_787"
}

Đošić, M., Eraković, S., Janković, A., Vukasinović-Sekulić, M., Matić, I. Z., Stojanović, J., Rhee, K. Y., Mišković-Stanković, V.,& Park, S.. (2017). In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene. in Journal of Industrial and Engineering Chemistry
Elsevier Science Inc, New York., 47, 336-347.
https://doi.org/10.1016/j.jiec.2016.12.004
conv_787

Đošić M, Eraković S, Janković A, Vukasinović-Sekulić M, Matić IZ, Stojanović J, Rhee KY, Mišković-Stanković V, Park S. In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene. in Journal of Industrial and Engineering Chemistry. 2017;47:336-347.
doi:10.1016/j.jiec.2016.12.004
conv_787 .

Đošić, Marija, Eraković, Sanja, Janković, Ana, Vukasinović-Sekulić, Maja, Matić, Ivana Z., Stojanović, Jovica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, Park, Soo-Jin, "In vitro investigation of electrophoretically deposited bioactive hydroxyapatite/chitosan coatings reinforced by graphene" in Journal of Industrial and Engineering Chemistry, 47 (2017):336-347,
https://doi.org/10.1016/j.jiec.2016.12.004 .,
conv_787 .