Single-step, electrophoretically deposited Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications
Аутори
Jaćimović, NevenaĐošić, Marija
Janković, Ana
Grujić, Svetlana
Matić-Bujagić, Ivana
Stojanović, Jovica
Vukašinović-Sekulić, Maja
Kojić, Vesna
Mišković-Stanković, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion (XRD) after immersion in simulated body fluid (SBF) at 37°C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by th...e dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
Кључне речи:
antibacterial / gentamicin release / poly(vinyl alcohol) / chitosan / electrophoretic depositionИзвор:
Macedonian Journal of Chemistry and Chemical Engineering, 2023, 42, 2, 249-262Издавач:
- Skopje : Society of Chemists and Technologists of Macedonia
- Skopje : Ss. Cyril and Methodius University in Skopje
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200023 (Институт за технологију нуклеарних и других минералних сировина - ИТНМС, Београд) (RS-MESTD-inst-2020-200023)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- European Union’s Horizon 2020 research and innovation program under grant agreement No. 952033.
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
Институција/група
Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - JOUR AU - Jaćimović, Nevena AU - Đošić, Marija AU - Janković, Ana AU - Grujić, Svetlana AU - Matić-Bujagić, Ivana AU - Stojanović, Jovica AU - Vukašinović-Sekulić, Maja AU - Kojić, Vesna AU - Mišković-Stanković, Vesna PY - 2023 UR - https://ritnms.itnms.ac.rs/handle/123456789/1228 AB - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion (XRD) after immersion in simulated body fluid (SBF) at 37°C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes. PB - Skopje : Society of Chemists and Technologists of Macedonia PB - Skopje : Ss. Cyril and Methodius University in Skopje T2 - Macedonian Journal of Chemistry and Chemical Engineering T1 - Single-step, electrophoretically deposited Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications EP - 262 IS - 2 SP - 249 VL - 42 DO - 10.20450/mjcce.2023.2775 ER -
@article{ author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić-Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna", year = "2023", abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using the constant voltage method. Characterization of the deposited coatings was performed by scanning electron micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion (XRD) after immersion in simulated body fluid (SBF) at 37°C. The concentration of the released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.", publisher = "Skopje : Society of Chemists and Technologists of Macedonia, Skopje : Ss. Cyril and Methodius University in Skopje", journal = "Macedonian Journal of Chemistry and Chemical Engineering", title = "Single-step, electrophoretically deposited Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications", pages = "262-249", number = "2", volume = "42", doi = "10.20450/mjcce.2023.2775" }
Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić-Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering Skopje : Society of Chemists and Technologists of Macedonia., 42(2), 249-262. https://doi.org/10.20450/mjcce.2023.2775
Jaćimović N, Đošić M, Janković A, Grujić S, Matić-Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262. doi:10.20450/mjcce.2023.2775 .
Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić-Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262, https://doi.org/10.20450/mjcce.2023.2775 . .