Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants
Само за регистроване кориснике
2023
Аутори
Đošić, MarijaJanković, Ana
Stevanović, Milena
Stojanović, Jovica
Vukašinović-Sekulić, Maja
Kojić, Vesna
Mišković-Stanković, Vesna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants.
Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin
(Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral
composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with
improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration
of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent
coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric
analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP.
The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating
against Staphylococcus aureus and ...Escherichia coli. Cytotoxicity studies were performed towards human MRC-5
and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent)
expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was
investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent
to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents,
along with antibiotic gentamicin, improving the antibacterial coatings’ properties.
Кључне речи:
hydroxyapatite / poly(vinyl alcohol) / chitosan / gentamicin / antibacterial activityИзвор:
Materials Chemistry and Physics, 2023, 303, 127766Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200023 (Институт за технологију нуклеарних и других минералних сировина - ИТНМС, Београд) (RS-MESTD-inst-2020-200023)
- This research was funded by European Union’s Horizon 2020 research and innovation program under grant agreement No. 952033.
Институција/група
Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - JOUR AU - Đošić, Marija AU - Janković, Ana AU - Stevanović, Milena AU - Stojanović, Jovica AU - Vukašinović-Sekulić, Maja AU - Kojić, Vesna AU - Mišković-Stanković, Vesna PY - 2023 UR - https://ritnms.itnms.ac.rs/handle/123456789/666 AB - Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants. Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin (Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP. The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5 and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent) expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents, along with antibiotic gentamicin, improving the antibacterial coatings’ properties. PB - Elsevier T2 - Materials Chemistry and Physics T1 - Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants IS - 127766 VL - 303 DO - 10.1016/j.matchemphys.2023.127766 ER -
@article{ author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna", year = "2023", abstract = "Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants. Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin (Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP. The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5 and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent) expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents, along with antibiotic gentamicin, improving the antibacterial coatings’ properties.", publisher = "Elsevier", journal = "Materials Chemistry and Physics", title = "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants", number = "127766", volume = "303", doi = "10.1016/j.matchemphys.2023.127766" }
Đošić, M., Janković, A., Stevanović, M., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics Elsevier., 303(127766). https://doi.org/10.1016/j.matchemphys.2023.127766
Đošić M, Janković A, Stevanović M, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics. 2023;303(127766). doi:10.1016/j.matchemphys.2023.127766 .
Đošić, Marija, Janković, Ana, Stevanović, Milena, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants" in Materials Chemistry and Physics, 303, no. 127766 (2023), https://doi.org/10.1016/j.matchemphys.2023.127766 . .