Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
Аутори
Stevanović, MilenaĐošić, Marija
Janković, Ana
Sprecher, Christoph Martin
Vukašinović-Sekulić, Maja
Mišković-Stanković, Vesna
Остала ауторства
Obradović, BojanaКонференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray... spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.
Кључне речи:
composite coatings / hydroxyapatite / gentamicin / titaniumИзвор:
ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 2024, 78, 1s, 58-58Издавач:
- Beograd : Savez hemijskih inženjera Srbije
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200023 (Институт за технологију нуклеарних и других минералних сировина - ИТНМС, Београд) (RS-MESTD-inst-2020-200023)
- European Union’s Horizon 2020 research and innovation program under grant agreement No. 952033.
Институција/група
Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - CONF AU - Stevanović, Milena AU - Đošić, Marija AU - Janković, Ana AU - Sprecher, Christoph Martin AU - Vukašinović-Sekulić, Maja AU - Mišković-Stanković, Vesna PY - 2024 UR - https://ritnms.itnms.ac.rs/handle/123456789/1243 AB - INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/ chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1]. EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max, Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC 25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis of variance (ANOVA), followed by multiple comparisons post-hoc test. RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites. Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent. CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications. HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects. The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for biomedical applications. PB - Beograd : Savez hemijskih inženjera Srbije C3 - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices T1 - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach EP - 58 IS - 1s SP - 58 VL - 78 ER -
@conference{ author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna", year = "2024", abstract = "INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/ chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1]. EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max, Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC 25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis of variance (ANOVA), followed by multiple comparisons post-hoc test. RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites. Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent. CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications. HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects. The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for biomedical applications.", publisher = "Beograd : Savez hemijskih inženjera Srbije", journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices", title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach", pages = "58-58", number = "1s", volume = "78" }
Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices Beograd : Savez hemijskih inženjera Srbije., 78(1s), 58-58.
Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):58-58..
Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):58-58.