TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1244
AB  - INTRODUCTION: Following orthopedic surgery, bacterial infection may lead to significant complications related to
inflammation in the peri-implant region, including the potential for implant loosening. To address this concern, the
surface of metallic implants was modified through the application of bioactive and antibacterial coatings, aiming to
mitigate these issues.
EXPERIMENTAL: HAP/PVA/CS/Gent coatings was single-step electrophoretically deposited from four-component
aqueous suspension containing 1 wt.% hydroxyapatite powder (HAP, particles < 200 nm particle size, Sigma-Aldrich),
0.1 wt.% poly(vinyl alcohol) (PVA, medium molecular weight 89 to 98 kDa, 99 % hydrolysed, Sigma-Aldrich), 0.05 wt%
chitosan powder (CS, medium molecular weight, 190 to 310kDa with 75 to 85 % deacetylation degree, Sigma-Aldrich), and
aqueous gentamicin sulfate solution (Gent, concentration 50 mg/ml, Sigma-Aldrich) on titanium plates (Sigma-Aldrich).
Antibacterial activity of HAP/PVA/CS/Gent coating was evaluated against Staphylococcus aureus TL (culture collection-
FTM, University of Belgrade, Serbia) and Escherichia coli ATCC 25922, while kinetics of antibacterial activity was monitored
according to our previously published data [1]. In vitro cytotoxicity assay was performed towards two fibroblast cell lines
of different origin-mouse origin cell line (L929 (ATCC CRL-6364)) and human lung origin cell line (MRC-5 (ATCC CCL-171)) [2].
To evaluate the statistical significance of the biological assay results (antibacterial activity, cytotoxicity and ALP activity),
one-way analysis of variance (ANOVA) followed by a multiple comparisons posthoc test was used.
RESULTS AND DISCUSSION: HAP/PVA/CS/Gent coating exhibited strong antibacterial effect against both S. aureus
and E. coli, especially pronounced against S. aureus, causing bactericidal effect. Cytotoxic effect of HAP/PVA/CS/Gent
coating was not pronounced in investigated MRC-5 and L929 cell lines. MRC-5 fibroblast cells in contact with
HAP/PVA/CS/Gent doubled alkaline phosphatase levels compared to their contact with the control samples
(HAP/PVA/CS), indicating good osteogenic properties.
CONCLUSIONS: Electrophoretically deposited HAP/PVA/CS/Gent bioceramic coatings on titanium, demonstrated
significant potential as implants in orthopedic practice, functioning as drug carriers. Not only do they possess
antibacterial properties, but they also exhibit no adverse effects on living tissue.
PB  - Beograd : Savez hemijskih inženjera Srbije
C3  - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
T1  - Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application
EP  - 59
IS  - 1s
SP  - 59
VL  - 78
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Đošić, Marija and Janković, Ana and Stevanović, Milena and Grujić, Svetlana and Matić-Bujagić, Ivana and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2024",
abstract = "INTRODUCTION: Following orthopedic surgery, bacterial infection may lead to significant complications related to
inflammation in the peri-implant region, including the potential for implant loosening. To address this concern, the
surface of metallic implants was modified through the application of bioactive and antibacterial coatings, aiming to
mitigate these issues.
EXPERIMENTAL: HAP/PVA/CS/Gent coatings was single-step electrophoretically deposited from four-component
aqueous suspension containing 1 wt.% hydroxyapatite powder (HAP, particles < 200 nm particle size, Sigma-Aldrich),
0.1 wt.% poly(vinyl alcohol) (PVA, medium molecular weight 89 to 98 kDa, 99 % hydrolysed, Sigma-Aldrich), 0.05 wt%
chitosan powder (CS, medium molecular weight, 190 to 310kDa with 75 to 85 % deacetylation degree, Sigma-Aldrich), and
aqueous gentamicin sulfate solution (Gent, concentration 50 mg/ml, Sigma-Aldrich) on titanium plates (Sigma-Aldrich).
Antibacterial activity of HAP/PVA/CS/Gent coating was evaluated against Staphylococcus aureus TL (culture collection-
FTM, University of Belgrade, Serbia) and Escherichia coli ATCC 25922, while kinetics of antibacterial activity was monitored
according to our previously published data [1]. In vitro cytotoxicity assay was performed towards two fibroblast cell lines
of different origin-mouse origin cell line (L929 (ATCC CRL-6364)) and human lung origin cell line (MRC-5 (ATCC CCL-171)) [2].
To evaluate the statistical significance of the biological assay results (antibacterial activity, cytotoxicity and ALP activity),
one-way analysis of variance (ANOVA) followed by a multiple comparisons posthoc test was used.
RESULTS AND DISCUSSION: HAP/PVA/CS/Gent coating exhibited strong antibacterial effect against both S. aureus
and E. coli, especially pronounced against S. aureus, causing bactericidal effect. Cytotoxic effect of HAP/PVA/CS/Gent
coating was not pronounced in investigated MRC-5 and L929 cell lines. MRC-5 fibroblast cells in contact with
HAP/PVA/CS/Gent doubled alkaline phosphatase levels compared to their contact with the control samples
(HAP/PVA/CS), indicating good osteogenic properties.
CONCLUSIONS: Electrophoretically deposited HAP/PVA/CS/Gent bioceramic coatings on titanium, demonstrated
significant potential as implants in orthopedic practice, functioning as drug carriers. Not only do they possess
antibacterial properties, but they also exhibit no adverse effects on living tissue.",
publisher = "Beograd : Savez hemijskih inženjera Srbije",
journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices",
title = "Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application",
pages = "59-59",
number = "1s",
volume = "78"
}

Vukašinović-Sekulić, M., Đošić, M., Janković, A., Stevanović, M., Grujić, S., Matić-Bujagić, I., Kojić, V.,& Mišković-Stanković, V.. (2024). Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
Beograd : Savez hemijskih inženjera Srbije., 78(1s), 59-59.

Vukašinović-Sekulić M, Đošić M, Janković A, Stevanović M, Grujić S, Matić-Bujagić I, Kojić V, Mišković-Stanković V. Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):59-59..

Vukašinović-Sekulić, Maja, Đošić, Marija, Janković, Ana, Stevanović, Milena, Grujić, Svetlana, Matić-Bujagić, Ivana, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactivity of gentamicin-loaded hydroxyapatite/poly(vinyl alcohol)/chitosan composite coatings aimed for orthopedic application" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):59-59.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Sprecher, Christoph Martin
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1243
AB  - INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.
PB  - Beograd : Savez hemijskih inženjera Srbije
C3  - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
T1  - Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach
EP  - 58
IS  - 1s
SP  - 58
VL  - 78
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Sprecher, Christoph Martin and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2024",
abstract = "INTRODUCTION: Tissue engineering strives for innovative solutions in addressing challenges associated with
contemporary bone tissue implants. This study focuses on the electrophoretic deposition (EPD) of hydroxyapatite-based
bioceramic composites containing antibacterial agents onto titanium surfaces. Two composite coatings, hydroxyapatite/
chitosan (HAP/CS) and hydroxyapatite/chitosan/gentamicin (HAP/CS/Gent) were developed to combat issues such as
poor adhesion, limited antibacterial potential, limited bioactivity, and potential toxicity of implant materials [1].
EXPERIMENTAL: EPD was performed at constant voltage (5 V, 12 min) on pure Ti plates from aqueous (HAP/CS and
HAP/CS/Gent) suspensions. The uniformity and functionality of the deposited coatings were assessed through
comprehensive physico-chemical characterization using X-ray diffraction (XRD) (Philips PW 1710, Netherlands) and
scanning electron microscopy (SEM, Hitachi S-4700, J) equipped with energy dispersive X-Ray spectroscopy (EDS, X-Max,
Oxford Instruments, UK). Antibacterial activity was evaluated against Staphylococcus aureus TL and Escherichia coli ATCC
25922 by quantitatively monitoring changes in the viable number of bacterial cells in suspension. Cytotoxicity against
MRC-5 and L929 cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell
metabolic activity. Statistical significance was determined for antibacterial and cytotoxicity results by one-way analysis
of variance (ANOVA), followed by multiple comparisons post-hoc test.
RESULTS AND DISCUSSION: XRD revealed broadened diffraction maximums corresponding to fine HAP crystallites.
Porous surface with homogeneously distributed spherical HAP agglomerates embedded in wax-like polymers' matrix of
CS was observed for both coatings by SEM. The addition of gentamicin significantly enhanced the antibacterial activity
of the HAP/CS/Gent coating – complete reduction of S. aureus bacterial cells was achieved within 1 h of exposure. MTT
and DET tests indicated low cytotoxicity against MRC-5 and L929 tested cell line for both samples. Slightly decreased
cell percentage viability due to gentamicin presence was observed for HAP/CS/Gent.
CONCLUSIONS: Single-step EPD yielded antibacterial composite coatings with potential for biomedical applications.
HAP/CS/Gent showed successful gentamicin loading, favorable crystalline structure, and strong antibacterial effects.
The presence of fine HAP crystallites yielded a larger surface area, favorable for new bone growth and improved
osseointegration. HAP/CS/Gent exhibited good antibacterial activity against both tested bacteria (especially
pronounced against S. aureus – bactericidal effect), while preserving low cytotoxicity, indicating the high potential for
biomedical applications.",
publisher = "Beograd : Savez hemijskih inženjera Srbije",
journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices",
title = "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach",
pages = "58-58",
number = "1s",
volume = "78"
}

Stevanović, M., Đošić, M., Janković, A., Sprecher, C. M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices
Beograd : Savez hemijskih inženjera Srbije., 78(1s), 58-58.

Stevanović M, Đošić M, Janković A, Sprecher CM, Vukašinović-Sekulić M, Mišković-Stanković V. Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices. 2024;78(1s):58-58..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Sprecher, Christoph Martin, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Innovative hydroxyapatite-based coatings for bone implants: A multifaceted approach" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, 78, no. 1s (2024):58-58.

TY  - JOUR
AU  - Mišković-Stanković, Vesna
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Radojević, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Đošić, Marija
AU  - Atanasković, Teodor
PY  - 2024
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1229
AB  - This study presents comparison of our recently formulated two compartmental model with General fractional derivative (GFD) and Korsmeyer-Peppas, Makoid-Banakar and Kopcha diffusion models. We have used our GFD
model to study the release of gentamicin in poly (vinylalcohol)/chitosan/gentamicin (PVA/CHI/Gent) hydrogel aimed for wound dressing in medical treatment of deep chronical wounds. The PVA/CHI/Gent hydrogel was prepared by physical cross linking of poly(vinyl alcohol)/chitosan dispersion using freezing-thawing method, and then was swollen for 48 h in gentamicin solution, at 37 °C. Different physico-chemical (FTIR, SEM), mechanical and biological (cytotoxicity, antibacterial activity) properties have been determined. The concentration of released gentamicin was determined
using a high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS). The ratio between concentration of released gentamicin and initial concentration of gentamicin in the hydrogel was monitored for the
prolonged time period in order to obtain gentamicin release profile. It was proven that our novel diffusion GFD model better fitted with experimental data, and enabled the determination of diffusion coefficient precisely for the entire time period.
PB  - Belgrade :  Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel
DO  - 10.2298/JSC231207010M
ER  - 

@article{
author = "Mišković-Stanković, Vesna and Janković, Ana and Grujić, Svetlana and Matić-Bujagić, Ivana and Radojević, Vesna and Vukašinović-Sekulić, Maja and Kojić, Vesna and Đošić, Marija and Atanasković, Teodor",
year = "2024",
abstract = "This study presents comparison of our recently formulated two compartmental model with General fractional derivative (GFD) and Korsmeyer-Peppas, Makoid-Banakar and Kopcha diffusion models. We have used our GFD
model to study the release of gentamicin in poly (vinylalcohol)/chitosan/gentamicin (PVA/CHI/Gent) hydrogel aimed for wound dressing in medical treatment of deep chronical wounds. The PVA/CHI/Gent hydrogel was prepared by physical cross linking of poly(vinyl alcohol)/chitosan dispersion using freezing-thawing method, and then was swollen for 48 h in gentamicin solution, at 37 °C. Different physico-chemical (FTIR, SEM), mechanical and biological (cytotoxicity, antibacterial activity) properties have been determined. The concentration of released gentamicin was determined
using a high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS). The ratio between concentration of released gentamicin and initial concentration of gentamicin in the hydrogel was monitored for the
prolonged time period in order to obtain gentamicin release profile. It was proven that our novel diffusion GFD model better fitted with experimental data, and enabled the determination of diffusion coefficient precisely for the entire time period.",
publisher = "Belgrade :  Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel",
doi = "10.2298/JSC231207010M"
}

Mišković-Stanković, V., Janković, A., Grujić, S., Matić-Bujagić, I., Radojević, V., Vukašinović-Sekulić, M., Kojić, V., Đošić, M.,& Atanasković, T.. (2024). Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel. in Journal of the Serbian Chemical Society
Belgrade :  Serbian Chemical Society..
https://doi.org/10.2298/JSC231207010M

Mišković-Stanković V, Janković A, Grujić S, Matić-Bujagić I, Radojević V, Vukašinović-Sekulić M, Kojić V, Đošić M, Atanasković T. Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel. in Journal of the Serbian Chemical Society. 2024;.
doi:10.2298/JSC231207010M .

Mišković-Stanković, Vesna, Janković, Ana, Grujić, Svetlana, Matić-Bujagić, Ivana, Radojević, Vesna, Vukašinović-Sekulić, Maja, Kojić, Vesna, Đošić, Marija, Atanasković, Teodor, "Diffusion models of gentamicin released in poly(vinyl alcohol)/chitosan hydrogel" in Journal of the Serbian Chemical Society (2024),
https://doi.org/10.2298/JSC231207010M . .

TY  - JOUR
AU  - Jaćimović, Nevena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/1228
AB  - Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using  the  constant voltage  method. Characterization of  the  deposited coatings  was performed by scanning electron  micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion  (XRD)  after  immersion  in  simulated  body  fluid  (SBF)  at  37°C.  The  concentration  of  the  released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.
PB  - Skopje : Society of Chemists and Technologists of Macedonia
PB  - Skopje : Ss. Cyril and Methodius University in Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications
EP  - 262
IS  - 2
SP  - 249
VL  - 42
DO  - 10.20450/mjcce.2023.2775
ER  - 

@article{
author = "Jaćimović, Nevena and Đošić, Marija and Janković, Ana and Grujić, Svetlana and Matić-Bujagić, Ivana and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Biocomposite hydroxyapatite/poly(vinyl alcohol)/chitosan/gentamicincoatings were fabricated on titanium by an electrophoretic deposition process (EPD) from an aqueous suspension using  the  constant voltage  method. Characterization of  the  deposited coatings  was performed by scanning electron  micros-copy with field emission (FE–SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffrac-tion  (XRD)  after  immersion  in  simulated  body  fluid  (SBF)  at  37°C.  The  concentration  of  the  released gentamicin was determined using high-performance liquid chromatography (HPLC) coupled with a mass spectrometer (MS). The release profile confirmed an initial “burst-release effect”with ~ 30% of the gen-tamicin released in the first 48 h, which could be of assistance in the prevention of biofilm formation. An-tibacterial activity was proven by the agar diffusion method on Staphylococcus aureusTL and Escherich-ia coliATCC 25922 bacterial strains. Cytotoxicity was tested by the dye exclusion test (DET) on MRC-5 and L929 fibroblast cell lines, with both coatings expressing nontoxicity. The results showed the high ap-plicability potential of a poly(vinyl alcohol)-based biocomposite coating for medical purposes.",
publisher = "Skopje : Society of Chemists and Technologists of Macedonia, Skopje : Ss. Cyril and Methodius University in Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications",
pages = "262-249",
number = "2",
volume = "42",
doi = "10.20450/mjcce.2023.2775"
}

Jaćimović, N., Đošić, M., Janković, A., Grujić, S., Matić-Bujagić, I., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering
Skopje : Society of Chemists and Technologists of Macedonia., 42(2), 249-262.
https://doi.org/10.20450/mjcce.2023.2775

Jaćimović N, Đošić M, Janković A, Grujić S, Matić-Bujagić I, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):249-262.
doi:10.20450/mjcce.2023.2775 .

Jaćimović, Nevena, Đošić, Marija, Janković, Ana, Grujić, Svetlana, Matić-Bujagić, Ivana, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Single-step, electrophoretically deposited  Hydroxyapatite/Poly(Vinyl Alcohol)/Chitosan/Gentamicin coating for biomedical applications" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):249-262,
https://doi.org/10.20450/mjcce.2023.2775 . .

TY  - JOUR
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Stojanović, Jovica
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/666
AB  - Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants.
Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin
(Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral
composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with
improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration
of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent
coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric
analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP.
The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating
against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5
and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent)
expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was
investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent
to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents,
along with antibiotic gentamicin, improving the antibacterial coatings’ properties.
PB  - Elsevier
T2  - Materials Chemistry and Physics
T1  - Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants
IS  - 127766
VL  - 303
DO  - 10.1016/j.matchemphys.2023.127766
ER  - 

@article{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Stojanović, Jovica and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Electrophoretic deposition (EPD) was employed to fabricate composite coatings aimed for orthopedic implants.
Hydroxyapatite (HAP), poly(vinyl alcohol) (PVA) and chitosan (CS) coatings, with or without antibiotic gentamicin
(Gent) were deposited in a single-step process from aqueous suspension, using EPD. Polymer/mineral
composite coatings were formed with the aim to mimic the natural composition of bone tissue, along with
improved coatings antibacterial properties, at the same time serving as a drug carrier, allowing local administration
of antibiotic. Structural and morphological characterization of HAP/PVA/CS and HAP/PVA/CS/Gent
coatings was investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric
analysis (TG/DTG), and scanning electron microscopy (SEM) proving carbonate-substituted HAP.
The presence of gentamicin contributed to the bactericidal activity of composite HAP/PVA/CS/Gent coating
against Staphylococcus aureus and Escherichia coli. Cytotoxicity studies were performed towards human MRC-5
and mice L929 fibroblast cell lines, and both investigated samples (HAP/PVA/CS and HAP/PVA/CS/Gent)
expressed good biocompatibility, without causing toxic effect on tested cells. Biomineralization potential was
investigated by measuring the alkaline phosphatase activity, demonstrating high potential of HAP/PVA/CS/Gent
to induce the growth of new HAP layer. Therefore, the present investigation proposes the production of nents,
along with antibiotic gentamicin, improving the antibacterial coatings’ properties.",
publisher = "Elsevier",
journal = "Materials Chemistry and Physics",
title = "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants",
number = "127766",
volume = "303",
doi = "10.1016/j.matchemphys.2023.127766"
}

Đošić, M., Janković, A., Stevanović, M., Stojanović, J., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2023). Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics
Elsevier., 303(127766).
https://doi.org/10.1016/j.matchemphys.2023.127766

Đošić M, Janković A, Stevanović M, Stojanović J, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants. in Materials Chemistry and Physics. 2023;303(127766).
doi:10.1016/j.matchemphys.2023.127766 .

Đošić, Marija, Janković, Ana, Stevanović, Milena, Stojanović, Jovica, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Hydroxyapatite/poly(vinyl alcohol)/chitosan coating with gentamicin for orthopedic implants" in Materials Chemistry and Physics, 303, no. 127766 (2023),
https://doi.org/10.1016/j.matchemphys.2023.127766 . .

TY  - CONF
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/749
AB  - Uvođenje antibiotika u polimer/mineralnu kompozitnu prevlaku poboljšava antibakterijska svojstva prevlaka. Kompozitna prevlaka (hidroksiapatit - HAP, poli(vinilalkohol), hitozan, gentamicin) dobijena je elektroforetskim taloženjem iz četvorokomponentne vodene suspenzije. Primenom termogravimetrijske i rendgenske difrakcione analize pokazano je prisustvo karbonatno-supstituisanog HAP. Antibakterijska aktivnost kompozitne prevlake sa gentamicinom potvrđena je protiv soja S. aureus i E. coli, čineći ovu prevlaku pogodnom za potencijalnu primenu u biomedicini.
AB  - Antibiotic introduction in polymer/mineral coating can improve the antibacterial properties of coatings. Composite coating (hydroxyapatite, poly(vinyl alcohol), chitosan, gentamicin) was fabricated from a four-component aqueous suspension using electrophoretic deposition. Structural and morphological characterization of the coating was investigated by X-ray diffraction and thermogravimetric analysis proving carbonate-substituted HAP. The presence of gentamicin contributed to the antibacterial activity of composite coating against S. aureus and E. coli strains, representing a suitable material for possible application in biomedicine.
PB  - Belgrade : Serbian Chemical Society
C3  - 59. Savetovanje Srpskog hemijskog društva
T1  - Elektroforetski taložene kompozitne prevlake sa gentamicinom
T1  - Electrophoretically deposited gentamicin-loaded composite coating
EP  - 58
SP  - 58
ER  - 

@conference{
author = "Đošić, Marija and Janković, Ana and Stevanović, Milena and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2023",
abstract = "Uvođenje antibiotika u polimer/mineralnu kompozitnu prevlaku poboljšava antibakterijska svojstva prevlaka. Kompozitna prevlaka (hidroksiapatit - HAP, poli(vinilalkohol), hitozan, gentamicin) dobijena je elektroforetskim taloženjem iz četvorokomponentne vodene suspenzije. Primenom termogravimetrijske i rendgenske difrakcione analize pokazano je prisustvo karbonatno-supstituisanog HAP. Antibakterijska aktivnost kompozitne prevlake sa gentamicinom potvrđena je protiv soja S. aureus i E. coli, čineći ovu prevlaku pogodnom za potencijalnu primenu u biomedicini., Antibiotic introduction in polymer/mineral coating can improve the antibacterial properties of coatings. Composite coating (hydroxyapatite, poly(vinyl alcohol), chitosan, gentamicin) was fabricated from a four-component aqueous suspension using electrophoretic deposition. Structural and morphological characterization of the coating was investigated by X-ray diffraction and thermogravimetric analysis proving carbonate-substituted HAP. The presence of gentamicin contributed to the antibacterial activity of composite coating against S. aureus and E. coli strains, representing a suitable material for possible application in biomedicine.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59. Savetovanje Srpskog hemijskog društva",
title = "Elektroforetski taložene kompozitne prevlake sa gentamicinom, Electrophoretically deposited gentamicin-loaded composite coating",
pages = "58-58"
}

Đošić, M., Janković, A., Stevanović, M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2023). Elektroforetski taložene kompozitne prevlake sa gentamicinom. in 59. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 58-58.

Đošić M, Janković A, Stevanović M, Vukašinović-Sekulić M, Mišković-Stanković V. Elektroforetski taložene kompozitne prevlake sa gentamicinom. in 59. Savetovanje Srpskog hemijskog društva. 2023;:58-58..

Đošić, Marija, Janković, Ana, Stevanović, Milena, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektroforetski taložene kompozitne prevlake sa gentamicinom" in 59. Savetovanje Srpskog hemijskog društva (2023):58-58.

TY  - CONF
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/753
AB  - Multifunkcionalni i višekomponentni biomaterijali se cesto primenjuju u rekonstruktivnoj
hirurgiji zbog sposobnosti da pospešuju osteointegraciju, istovremeno omogucavajuci
primenu antibakterijskih agenasa direktno na mestu intervencije. Primenom
elektroforetskog taloženja, iz cetvorokomponentne vodene suspenzije (hidroksiapatit,
poli(vinilalkohol), hitozan i gentamicin) dobijene su, u jednom koraku, bioaktivne i
antibakterijske kompozitne prevlake na titanu. Karakterizacija prevlaka je izvršena
primenom rendgenske difrakcione analize (XRD), infracrvene spektroskopije sa
Furijeovom transformacijom (FTIR) i skenirajuce elektronske mikroskopije (SEM).
Pracenjem kinetike antibakterijske aktivnosti u suspenziji potvr ena je antibakterijska
efikasnost prevlake sa gentamicinom. Prema dobijenim rezultatima moguce je zakljuciti da
dobijene kompozitne prevlake sa gentamicinom predstavljaju uspešan biomaterijal za
potencijalnu primenu u biomedicini.
AB  - Multifunctional and multicomponent biomaterials are very often the material of choice in
reconstructive surgery, having the ability to promote osteointegration, allowing the
antibacterial agents application directly at the site of intervention. Single-step
electrophoretic deposition was employed to assemble bioactive and antibacterial composite
coatings on titanium, from a four-component aqueous suspension (hydroxyapatite,
poly(vinyl alcohol), chitosan and gentamicin). Physico-chemical characterization of the
coatings was performed using X-ray diffraction (XRD), Fourier transform infrared
spectroscopy (FTIR) and scanning electron microscopy (SEM). Antibacterial activity of
gentamicin loaded coating was confirmed by antibacterial activity kinetics in suspension.
According to the obtained results, it can be concluded that composite coating loaded with
gentamicin represents a successful biomaterial for possible application in biomedicine.
PB  - Belgrade : Serbian Chemical Society
C3  - 58. Savetovanje Srpskog hemijskog društva
T1  - Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium
EP  - 71
SP  - 71
ER  - 

@conference{
author = "Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Multifunkcionalni i višekomponentni biomaterijali se cesto primenjuju u rekonstruktivnoj
hirurgiji zbog sposobnosti da pospešuju osteointegraciju, istovremeno omogucavajuci
primenu antibakterijskih agenasa direktno na mestu intervencije. Primenom
elektroforetskog taloženja, iz cetvorokomponentne vodene suspenzije (hidroksiapatit,
poli(vinilalkohol), hitozan i gentamicin) dobijene su, u jednom koraku, bioaktivne i
antibakterijske kompozitne prevlake na titanu. Karakterizacija prevlaka je izvršena
primenom rendgenske difrakcione analize (XRD), infracrvene spektroskopije sa
Furijeovom transformacijom (FTIR) i skenirajuce elektronske mikroskopije (SEM).
Pracenjem kinetike antibakterijske aktivnosti u suspenziji potvr ena je antibakterijska
efikasnost prevlake sa gentamicinom. Prema dobijenim rezultatima moguce je zakljuciti da
dobijene kompozitne prevlake sa gentamicinom predstavljaju uspešan biomaterijal za
potencijalnu primenu u biomedicini., Multifunctional and multicomponent biomaterials are very often the material of choice in
reconstructive surgery, having the ability to promote osteointegration, allowing the
antibacterial agents application directly at the site of intervention. Single-step
electrophoretic deposition was employed to assemble bioactive and antibacterial composite
coatings on titanium, from a four-component aqueous suspension (hydroxyapatite,
poly(vinyl alcohol), chitosan and gentamicin). Physico-chemical characterization of the
coatings was performed using X-ray diffraction (XRD), Fourier transform infrared
spectroscopy (FTIR) and scanning electron microscopy (SEM). Antibacterial activity of
gentamicin loaded coating was confirmed by antibacterial activity kinetics in suspension.
According to the obtained results, it can be concluded that composite coating loaded with
gentamicin represents a successful biomaterial for possible application in biomedicine.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "58. Savetovanje Srpskog hemijskog društva",
title = "Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium",
pages = "71-71"
}

Đošić, M., Janković, A., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2022). Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium. in 58. Savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 71-71.

Đošić M, Janković A, Vukašinović-Sekulić M, Mišković-Stanković V. Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium. in 58. Savetovanje Srpskog hemijskog društva. 2022;:71-71..

Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Kompozitne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu / Composite bioactive coatings loaded with gentamicin electrophoretically deposited on titanium" in 58. Savetovanje Srpskog hemijskog društva (2022):71-71.

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/752
AB  - Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Twentieth Young Researchers Conference – Materials Science and Engineering
T1  - Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium
EP  - 15
SP  - 15
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2022",
abstract = "Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Twentieth Young Researchers Conference – Materials Science and Engineering",
title = "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium",
pages = "15-15"
}

Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2022). Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 15-15.

Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering. 2022;:15-15..

Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium" in Twentieth Young Researchers Conference – Materials Science and Engineering (2022):15-15.

TY  - CONF
AU  - Janković, Ana
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Grujić, Svetlana
AU  - Matić-Bujagić, Ivana
AU  - Mišković-Stanković, Vesna
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/759
AB  - Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene.
AB  - Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.
PB  - Belgrade : Serbian Chemical Society
C3  - 57. savetovanje Srpskog hemijskog društva
T1  - Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium
EP  - 46
SP  - 46
ER  - 

@conference{
author = "Janković, Ana and Stevanović, Milena and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Grujić, Svetlana and Matić-Bujagić, Ivana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene., Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "57. savetovanje Srpskog hemijskog društva",
title = "Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium",
pages = "46-46"
}

Janković, A., Stevanović, M., Đošić, M., Vukašinović-Sekulić, M., Kojić, V., Grujić, S., Matić-Bujagić, I.,& Mišković-Stanković, V.. (2021). Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium. in 57. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 46-46.

Janković A, Stevanović M, Đošić M, Vukašinović-Sekulić M, Kojić V, Grujić S, Matić-Bujagić I, Mišković-Stanković V. Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium. in 57. savetovanje Srpskog hemijskog društva. 2021;:46-46..

Janković, Ana, Stevanović, Milena, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Grujić, Svetlana, Matić-Bujagić, Ivana, Mišković-Stanković, Vesna, "Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium" in 57. savetovanje Srpskog hemijskog društva (2021):46-46.

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Rhee, Kyong Yop
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://ritnms.itnms.ac.rs/handle/123456789/755
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley
T2  - Journal of Biomedical Materials Research
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
EP  - 2189
IS  - 11
SP  - 2175
VL  - 108
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley",
journal = "Journal of Biomedical Materials Research",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research
Wiley., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Rhee KY, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/777
AB  - Electrophoretic deposition (EPD) process was successfully used for
obtaining composite antibacterial coating of antibiotic gentamicin (Gent), natural polymer
chitosan (CS), and hydroxyapatite (HAP). EPD was carried out on pure titanium (Ti) plates in
a single step from three-component aqueous suspension. The gentamicin influence was
examined through detailed physico-chemical and biological characterization. The formation
of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface was confirmed by
X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier
transform infrared spectroscopy (FTIR) and X-ray photoelectron analysis (XPS). Composite
HAP/CS and HAP/CS/Gent coatings exhibited homogeneous, porous fracture-free surface,
suggesting strong interfacial interactions between HAP and CS. Biological assays pointed to
high potential of HAP/CS/Gent coating for medical applications. Test in suspension and agar
diffusion method indicated that the gentamicin addition strongly influenced the antibacterial
activity of HAP/CS/Gent against Staphylococcus aureus and Escherichia coli.
Biocompatibility assays were performed through MTT and DET tests, indicating low
cytotoxicity against MRC-5 and L929 tested cell lines. The coating ability to induce and
promote osteoconduction was proved by ALP assay suggesting that HAP/CS/Gent coating is
an excellent material for bone tissue engineering.
PB  - Targoviste : Valahia University Press
T2  - 9th International Conference of the Chemical Societies of the South-East European Countries
T1  - Gentamicin-loaded bioceramic composite coating aimed for biomedical use
EP  - S4_P_09
SP  - S4_P_09
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Electrophoretic deposition (EPD) process was successfully used for
obtaining composite antibacterial coating of antibiotic gentamicin (Gent), natural polymer
chitosan (CS), and hydroxyapatite (HAP). EPD was carried out on pure titanium (Ti) plates in
a single step from three-component aqueous suspension. The gentamicin influence was
examined through detailed physico-chemical and biological characterization. The formation
of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface was confirmed by
X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier
transform infrared spectroscopy (FTIR) and X-ray photoelectron analysis (XPS). Composite
HAP/CS and HAP/CS/Gent coatings exhibited homogeneous, porous fracture-free surface,
suggesting strong interfacial interactions between HAP and CS. Biological assays pointed to
high potential of HAP/CS/Gent coating for medical applications. Test in suspension and agar
diffusion method indicated that the gentamicin addition strongly influenced the antibacterial
activity of HAP/CS/Gent against Staphylococcus aureus and Escherichia coli.
Biocompatibility assays were performed through MTT and DET tests, indicating low
cytotoxicity against MRC-5 and L929 tested cell lines. The coating ability to induce and
promote osteoconduction was proved by ALP assay suggesting that HAP/CS/Gent coating is
an excellent material for bone tissue engineering.",
publisher = "Targoviste : Valahia University Press",
journal = "9th International Conference of the Chemical Societies of the South-East European Countries",
title = "Gentamicin-loaded bioceramic composite coating aimed for biomedical use",
pages = "S4_P_09-S4_P_09"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2019). Gentamicin-loaded bioceramic composite coating aimed for biomedical use. in 9th International Conference of the Chemical Societies of the South-East European Countries
Targoviste : Valahia University Press., S4_P_09-S4_P_09.

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Mišković-Stanković V. Gentamicin-loaded bioceramic composite coating aimed for biomedical use. in 9th International Conference of the Chemical Societies of the South-East European Countries. 2019;:S4_P_09-S4_P_09..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Gentamicin-loaded bioceramic composite coating aimed for biomedical use" in 9th International Conference of the Chemical Societies of the South-East European Countries (2019):S4_P_09-S4_P_09.

TY  - CONF
AU  - Janković, Ana
AU  - Nešović, Katarina
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/775
AB  - Elektrohemijske metode su korišćene za sintezu različitih kompozita namenjenih za medicinsku primenu kao antibakterijske prevlake za koštane titanske implantate ili kao visoko efikasni antibakterijski hidrogelovi za ubrzano zarastanje rana. Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene suspenzije na titanu. Sadržaj gentamicina je kvantifikovan pomoću tečne hromatografije sa ultravioletnom (UV) detekcijom. Prevlaka HAP/CS/Gent je pokazala dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene. Biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs) dobijen je in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema S.aureus i E.coli je potvrđena testom u suspenziji i agar-difuzionom metodom.
AB  - Electrochemical methods were used for the synthesis of various composites intended for medical use as antibacterial coatings for bone titanium implants or as highly effective antibacterial hydrogels for accelerated wound healing. Electrophoretic deposition (EPD) in a single step at the constant voltage was successfully used to obtain composite coating based on hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium. Gentamicin content was quantified by liquid chromatography with ultraviolet (UV) detection. HAP/CS/Gent coating exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications. Biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs) was produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against S.aureus and E.coli was confirmed by in suspension testing and by disc-diffusion method.
PB  - Belgrade : Serbian Chemical Society
C3  - 56. savetovanje Srpskog hemijskog društva
T1  - Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima
EP  - 28
SP  - 28
ER  - 

@conference{
author = "Janković, Ana and Nešović, Katarina and Stevanović, Milena and Đošić, Marija and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Elektrohemijske metode su korišćene za sintezu različitih kompozita namenjenih za medicinsku primenu kao antibakterijske prevlake za koštane titanske implantate ili kao visoko efikasni antibakterijski hidrogelovi za ubrzano zarastanje rana. Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene suspenzije na titanu. Sadržaj gentamicina je kvantifikovan pomoću tečne hromatografije sa ultravioletnom (UV) detekcijom. Prevlaka HAP/CS/Gent je pokazala dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene. Biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs) dobijen je in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema S.aureus i E.coli je potvrđena testom u suspenziji i agar-difuzionom metodom., Electrochemical methods were used for the synthesis of various composites intended for medical use as antibacterial coatings for bone titanium implants or as highly effective antibacterial hydrogels for accelerated wound healing. Electrophoretic deposition (EPD) in a single step at the constant voltage was successfully used to obtain composite coating based on hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium. Gentamicin content was quantified by liquid chromatography with ultraviolet (UV) detection. HAP/CS/Gent coating exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications. Biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs) was produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against S.aureus and E.coli was confirmed by in suspension testing and by disc-diffusion method.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "56. savetovanje Srpskog hemijskog društva",
title = "Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima",
pages = "28-28"
}

Janković, A., Nešović, K., Stevanović, M., Đošić, M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2019). Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima. in 56. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 28-28.

Janković A, Nešović K, Stevanović M, Đošić M, Vukašinović-Sekulić M, Mišković-Stanković V. Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima. in 56. savetovanje Srpskog hemijskog društva. 2019;:28-28..

Janković, Ana, Nešović, Katarina, Stevanović, Milena, Đošić, Marija, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektrohemijski sintetizovani kompoziti sa inkorporiranim antibakterijskim agensima" in 56. savetovanje Srpskog hemijskog društva (2019):28-28.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/774
AB  - U ovom radu su ispitivane kompozitne prevlake koje se sastoje od hidroksiapatita (HAP), hitozana (CS) i grafena (Gr), sa i bez gentamicina (Gent) dobijene postupkom elektroforetskog taloženja (EPD). Taloženje je izvedeno u jednom koraku iz četvorokomponentne vodene suspenzije (HAP/CS/Gr/Gent) pri konstantnom naponu na titanskim (Ti) pločicama. Uticaj Gr i Gent je detaljno istražen primenom različitih fizičko-hemijskih i bioloških analiza. Karakteristike dobijenih HAP/CS/Gr i HAP/CS/Gr/Gent prevlaka ispitivane su infracrvenom spektroskopijom sa Furijeovom transformacijom (FT-IR), skenirajućom elektronskom mikroskopijom (FE-SEM), rendgenskom fotoelektronskom spektroskopijom (XPS) i rendgenskom difrakcionom analizom (XRD). Ispitivanja antibakterijske aktivnosti prema Escherichia coli i Staphylococcus aureus izvršena su metodom agar difuzije i testa u suspenziji, a citotoksičnost MTT testom na ćelijskim linijama MRC-5 i L929. Dobijeni rezultati ukazali su na veliki potencijal elektroforetski istaloženih kompozita za medicinske primene.
AB  - In this work composite coatings consisting of hydroxyapatite (HAP), chitosan (CS) and graphene (Gr), with and without gentamicin (Gent) produced by electrophoretic deposition process (EPD) were investigated. Deposition was performed in a single step from the four-component aqueous suspension (HAP/CS/Gr/Gent) at a constant voltage on titanium (Ti) plates. The influence of Gr and Gent has been thoroughly explored using various physico-chemical and biological analyses. The characteristics of the obtained HAP/CS/Gr and HAP/CS/Gr/Gent coatings were investigated using Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), X-ray photoelectron analysis (XPS) and X-ray diffraction (XRD) techniques. Antibacterial efficacy assays against Escherichia coli and Staphylococcus aureus were performed by agar diffusion method and in suspension testing, while cytotoxicity was elucidated using MTT test toward MRC-5 and L929 cell lines. Obtained results pointed out high potential of electrodeposited composites for medical applications.
PB  - Belgrade : Serbian Chemical Society
C3  - 56. savetovanje Srpskog hemijskog društva
T1  - Elektroforetsko taloženje antibakterijske kompozitne prevlake hidroksiapatita sa hitozanom, grafenom i gentamicinom
EP  - 26
SP  - 26
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2019",
abstract = "U ovom radu su ispitivane kompozitne prevlake koje se sastoje od hidroksiapatita (HAP), hitozana (CS) i grafena (Gr), sa i bez gentamicina (Gent) dobijene postupkom elektroforetskog taloženja (EPD). Taloženje je izvedeno u jednom koraku iz četvorokomponentne vodene suspenzije (HAP/CS/Gr/Gent) pri konstantnom naponu na titanskim (Ti) pločicama. Uticaj Gr i Gent je detaljno istražen primenom različitih fizičko-hemijskih i bioloških analiza. Karakteristike dobijenih HAP/CS/Gr i HAP/CS/Gr/Gent prevlaka ispitivane su infracrvenom spektroskopijom sa Furijeovom transformacijom (FT-IR), skenirajućom elektronskom mikroskopijom (FE-SEM), rendgenskom fotoelektronskom spektroskopijom (XPS) i rendgenskom difrakcionom analizom (XRD). Ispitivanja antibakterijske aktivnosti prema Escherichia coli i Staphylococcus aureus izvršena su metodom agar difuzije i testa u suspenziji, a citotoksičnost MTT testom na ćelijskim linijama MRC-5 i L929. Dobijeni rezultati ukazali su na veliki potencijal elektroforetski istaloženih kompozita za medicinske primene., In this work composite coatings consisting of hydroxyapatite (HAP), chitosan (CS) and graphene (Gr), with and without gentamicin (Gent) produced by electrophoretic deposition process (EPD) were investigated. Deposition was performed in a single step from the four-component aqueous suspension (HAP/CS/Gr/Gent) at a constant voltage on titanium (Ti) plates. The influence of Gr and Gent has been thoroughly explored using various physico-chemical and biological analyses. The characteristics of the obtained HAP/CS/Gr and HAP/CS/Gr/Gent coatings were investigated using Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), X-ray photoelectron analysis (XPS) and X-ray diffraction (XRD) techniques. Antibacterial efficacy assays against Escherichia coli and Staphylococcus aureus were performed by agar diffusion method and in suspension testing, while cytotoxicity was elucidated using MTT test toward MRC-5 and L929 cell lines. Obtained results pointed out high potential of electrodeposited composites for medical applications.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "56. savetovanje Srpskog hemijskog društva",
title = "Elektroforetsko taloženje antibakterijske kompozitne prevlake hidroksiapatita sa hitozanom, grafenom i gentamicinom",
pages = "26-26"
}

Stevanović, M., Đošić, M., Janković, A., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2019). Elektroforetsko taloženje antibakterijske kompozitne prevlake hidroksiapatita sa hitozanom, grafenom i gentamicinom. in 56. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 26-26.

Stevanović M, Đošić M, Janković A, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Elektroforetsko taloženje antibakterijske kompozitne prevlake hidroksiapatita sa hitozanom, grafenom i gentamicinom. in 56. savetovanje Srpskog hemijskog društva. 2019;:26-26..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Elektroforetsko taloženje antibakterijske kompozitne prevlake hidroksiapatita sa hitozanom, grafenom i gentamicinom" in 56. savetovanje Srpskog hemijskog društva (2019):26-26.

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2019
UR  - https://ritnms.itnms.ac.rs/handle/123456789/773
AB  - Bone diseases and especially prevalent surgical procedures of total joint replacements in
aging population, cause the need for swift development of new biomaterials for bone tissue
implants. Nowadays, Ti implants are by far most commonly used in orthopedics, due to their
excellent mechanical properties and biocompatibility. Having in mind that biomaterials
should stimulate bone tissue regeneration while providing strong support, the Ti implant
surface requires modifications to facilitate improved osteointegration. In this work,
modification of the titanium surface was carried out by depositing novel composite
hydroxyapatite/chitosan/graphene (HAP/CS/Gr) and
hydroxyapatite/chitosan/graphene/gentamicin (HAP/CS/Gr/Gent) coatings. Deposition was
performed by single-step electrophoretic deposition (EPD) technique from aqueous
suspension at constant voltage on pure titanium plates. X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR) and field-emission scanning electron microscopy
(FE-SEM) were used to characterize obtained composites and to explore the graphene
influence on the composites properties. The results of all performed analyses confirmed the
formation of new composite HAP/CS/Gr and HAP/CS/Gr /Gent coatings on Ti. Antibacterial
activity kinetics against Staphylococcus aureus and Escherichia coli was performed in order
to evaluate the antibacterial properties of obtained composites. HAP/CS/Gr/Gent coating
exhibited excellent antibacterial properties, justifying the gentamicin addition to composites.
Encouraging results for potential applications were obtained for cytotoxicity testing towards
MRC-5 and L929 cell lines. Good biocompatibility of composites was proven towards both
cell lines, with slightly more pronounced effect for human MRC-5 cell line. Summarizing the
obtained results it could be safe to state that HAP/CS/Gr/Gent composite represents a
prospective material for bone implant coating.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Eighteenth Young Researchers Conference – Materials Science and Engineering
T1  - Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating
EP  - 18
SP  - 18
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2019",
abstract = "Bone diseases and especially prevalent surgical procedures of total joint replacements in
aging population, cause the need for swift development of new biomaterials for bone tissue
implants. Nowadays, Ti implants are by far most commonly used in orthopedics, due to their
excellent mechanical properties and biocompatibility. Having in mind that biomaterials
should stimulate bone tissue regeneration while providing strong support, the Ti implant
surface requires modifications to facilitate improved osteointegration. In this work,
modification of the titanium surface was carried out by depositing novel composite
hydroxyapatite/chitosan/graphene (HAP/CS/Gr) and
hydroxyapatite/chitosan/graphene/gentamicin (HAP/CS/Gr/Gent) coatings. Deposition was
performed by single-step electrophoretic deposition (EPD) technique from aqueous
suspension at constant voltage on pure titanium plates. X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR) and field-emission scanning electron microscopy
(FE-SEM) were used to characterize obtained composites and to explore the graphene
influence on the composites properties. The results of all performed analyses confirmed the
formation of new composite HAP/CS/Gr and HAP/CS/Gr /Gent coatings on Ti. Antibacterial
activity kinetics against Staphylococcus aureus and Escherichia coli was performed in order
to evaluate the antibacterial properties of obtained composites. HAP/CS/Gr/Gent coating
exhibited excellent antibacterial properties, justifying the gentamicin addition to composites.
Encouraging results for potential applications were obtained for cytotoxicity testing towards
MRC-5 and L929 cell lines. Good biocompatibility of composites was proven towards both
cell lines, with slightly more pronounced effect for human MRC-5 cell line. Summarizing the
obtained results it could be safe to state that HAP/CS/Gr/Gent composite represents a
prospective material for bone implant coating.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Eighteenth Young Researchers Conference – Materials Science and Engineering",
title = "Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating",
pages = "18-18"
}

Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2019). Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating. in Eighteenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 18-18.

Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating. in Eighteenth Young Researchers Conference – Materials Science and Engineering. 2019;:18-18..

Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Surface modification of Ti implants by electrophoretic deposition of hydroxyapatite/chitosan/graphene/gentamicin coating" in Eighteenth Young Researchers Conference – Materials Science and Engineering (2019):18-18.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/768
AB  - Titanium (Ti) is the most widely used implant material due to its high corrosion
resistance, and good mechanical properties. The biocompatibility and bioactivity of
titanium can be significantly improved by the modification of its surface through the
deposition of bioceramic coating on Ti [1]. Materials of choice were hydroxyapatite
(HAP), chitosan (CS) and gentamicin because of their good properties. HAP is well
known for its outstanding biocompatibility, chemical composition similar to natural
bone and its ability to promote osseointegration. Improvement of mechanical and
antimicrobial properties of the coating was achieved by addition of CS, biocompatible,
natural polymer [2]. In order to improve antibacterial properties, and to avoid
the occurrence of various inflammatory processes during implantation, a broad
spectrum antibiotic gentamicin was added [3]. The combination of good mechanical
properties of Ti, the bioactivity of HAP and good adhesion properties of CS enables
the production of bone tissue implants with improved biological functions.
Cathodic electrophoretic deposition process (EPD) was employed for assembling
composite HAP/CS and HAP/CS/Gent coating on Ti substrate (Figure 1.). EPD was
carried out at the constant voltage on pure Ti plates from an aqueous suspension.
The aim was to produce homogenous bioactive coatings with improved mechanical
properties and remarkable antibacterial effects. Obtained coatings were characterized
by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron  spectroscopy (XPS) and field emission scanning electron microscopy (FE-SEM).
Antibacterial properties of coatings were tested against two bacteria strains – Staphylococcus
aureus and Escherichia coli, by agar diffusion test. Evaluation of relative
cytotoxicity of obtained coatings was performed using MTT test. The ability of
coatings to promote osseointegration was tested using ALP assay.
Obtained results confirmed the successful deposition of HAP/CS and HAP/CS/Gent
coatings on Ti using EPD technique. Hydrogen bonding of functional groups of
CS and HAP was confirmed by FT-IR analysis which also revealed several bands
characteristic for CS. Due to low gentamicin content in HAP/CS/Gent coating, no
characteristic bands corresponding to gentamicin were detected. The presence of
gentamicin in HAP/CS/Gent was revealed by XPS analysis through the deconvolution
of C1s peak that indicated binding of gentamicin to the matrix. Addition of the
polymer significantly improved morphology, and bioactivity of thus formed
composite. Morphology examination of HAP/CS and HAP/CS/Gent is represented
in Figure 2. FE-SEM micrographs confirmed bioactivity of HAP/CS and HAP/CS/
Gent after immersion in SBF. The rapid formation of apatite in a relatively short time
(after only 7 days) showed coatings ability to promote bonding between natural bone
and implants. MTT assay for HAP/CS and HAP/CS/Gent indicated low cytotoxicity
against MRC-5 and L929 cell lines. Both composite coatings exhibited strong
antibacterial activity against S.aureus and E. coli, indicating the high potential for
biomedical applications. This effect was slightly more pronounced for the samples
tested against S. aureus, for both HAP/CS and HAP/CS/Gent coatings.
Excellent osteogenic properties, through the promotion of osteoblast differentiation
were confirmed by ALP assay. Results were much more pronounced for HAP/CS/
Gent coating. Therefore, HAP/CS and HAP/CS/Gent coatings can be considered as
an excellent promising candidate for biomedical hard tissue implants [4].
PB  - Belgrade : Serbian Academy of Sciences and Arts
C3  - First International Conference on Electron Microscopy ELMINA2018
T1  - Bioactive Hydroxyapatite/Chitosan/Gentamicin Composite Coating Electrodeposited on Titanium
EP  - 227
SP  - 225
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Titanium (Ti) is the most widely used implant material due to its high corrosion
resistance, and good mechanical properties. The biocompatibility and bioactivity of
titanium can be significantly improved by the modification of its surface through the
deposition of bioceramic coating on Ti [1]. Materials of choice were hydroxyapatite
(HAP), chitosan (CS) and gentamicin because of their good properties. HAP is well
known for its outstanding biocompatibility, chemical composition similar to natural
bone and its ability to promote osseointegration. Improvement of mechanical and
antimicrobial properties of the coating was achieved by addition of CS, biocompatible,
natural polymer [2]. In order to improve antibacterial properties, and to avoid
the occurrence of various inflammatory processes during implantation, a broad
spectrum antibiotic gentamicin was added [3]. The combination of good mechanical
properties of Ti, the bioactivity of HAP and good adhesion properties of CS enables
the production of bone tissue implants with improved biological functions.
Cathodic electrophoretic deposition process (EPD) was employed for assembling
composite HAP/CS and HAP/CS/Gent coating on Ti substrate (Figure 1.). EPD was
carried out at the constant voltage on pure Ti plates from an aqueous suspension.
The aim was to produce homogenous bioactive coatings with improved mechanical
properties and remarkable antibacterial effects. Obtained coatings were characterized
by Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron  spectroscopy (XPS) and field emission scanning electron microscopy (FE-SEM).
Antibacterial properties of coatings were tested against two bacteria strains – Staphylococcus
aureus and Escherichia coli, by agar diffusion test. Evaluation of relative
cytotoxicity of obtained coatings was performed using MTT test. The ability of
coatings to promote osseointegration was tested using ALP assay.
Obtained results confirmed the successful deposition of HAP/CS and HAP/CS/Gent
coatings on Ti using EPD technique. Hydrogen bonding of functional groups of
CS and HAP was confirmed by FT-IR analysis which also revealed several bands
characteristic for CS. Due to low gentamicin content in HAP/CS/Gent coating, no
characteristic bands corresponding to gentamicin were detected. The presence of
gentamicin in HAP/CS/Gent was revealed by XPS analysis through the deconvolution
of C1s peak that indicated binding of gentamicin to the matrix. Addition of the
polymer significantly improved morphology, and bioactivity of thus formed
composite. Morphology examination of HAP/CS and HAP/CS/Gent is represented
in Figure 2. FE-SEM micrographs confirmed bioactivity of HAP/CS and HAP/CS/
Gent after immersion in SBF. The rapid formation of apatite in a relatively short time
(after only 7 days) showed coatings ability to promote bonding between natural bone
and implants. MTT assay for HAP/CS and HAP/CS/Gent indicated low cytotoxicity
against MRC-5 and L929 cell lines. Both composite coatings exhibited strong
antibacterial activity against S.aureus and E. coli, indicating the high potential for
biomedical applications. This effect was slightly more pronounced for the samples
tested against S. aureus, for both HAP/CS and HAP/CS/Gent coatings.
Excellent osteogenic properties, through the promotion of osteoblast differentiation
were confirmed by ALP assay. Results were much more pronounced for HAP/CS/
Gent coating. Therefore, HAP/CS and HAP/CS/Gent coatings can be considered as
an excellent promising candidate for biomedical hard tissue implants [4].",
publisher = "Belgrade : Serbian Academy of Sciences and Arts",
journal = "First International Conference on Electron Microscopy ELMINA2018",
title = "Bioactive Hydroxyapatite/Chitosan/Gentamicin Composite Coating Electrodeposited on Titanium",
pages = "227-225"
}

Stevanović, M., Đošić, M., Janković, A., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2018). Bioactive Hydroxyapatite/Chitosan/Gentamicin Composite Coating Electrodeposited on Titanium. in First International Conference on Electron Microscopy ELMINA2018
Belgrade : Serbian Academy of Sciences and Arts., 225-227.

Stevanović M, Đošić M, Janković A, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Bioactive Hydroxyapatite/Chitosan/Gentamicin Composite Coating Electrodeposited on Titanium. in First International Conference on Electron Microscopy ELMINA2018. 2018;:225-227..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactive Hydroxyapatite/Chitosan/Gentamicin Composite Coating Electrodeposited on Titanium" in First International Conference on Electron Microscopy ELMINA2018 (2018):225-227.

TY  - CONF
AU  - Janković, Ana
AU  - Nešović, Katarina
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/769
AB  - Elektrohemijske metode su koris ćene za sintezu razlic itih kompozita namenjenih za aplikacije u medicini kao antibakterijske prevlake na koštanim titanskim implantatima ili kao visoko efikasni antimikrobni hidrogelovi za ubrzano zarastanje rana.
Prvi sintetisani materijal je kompozitna prevlaka hidroksiapatita (HAP) sa prirodnim polimerom hitozanom (CS) koja uključuje antibiotik gentamicin, dobijena metodom elektroforetskog taloz enja (EPD). EPD se izvodi pri konstantnom naponu na ploc icama c istog titana iz vodene suspenzije. Na ovaj nac in dobijena porozna homogena prevlaka ima poboljs anu mehanic ku c vrstoću i povoljne antibakterijske osobine. Prevlaka HAP/CS/Gent okarakterisana je difrakcijom X-zraka (XRD), infracrvenom spektroskopijom sa Furijeovom transformacijom (FT-IR) i skenirajućom elektronskom mikroskopijom (FE-SEM). Dobijeni rezultati potvrdili su formiranje nove kompozitne prevlake HAP/CS/Gent. Citotoksičnost prema dvema vrstama ćelijskih linija ispitana je koris ćenjem testa iskljuc ivanja tripan plave boje (DET) i MTT testa za procenu ćelijske metaboličke aktivnosti. Prevlaka sa antibiotikom (HAP/CS/Gent) pokazala je dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, dok je istovremeno zadržala nisku citotoksičnost, što ukazuje na visok potencijal za biomedicinske primene.
Drugi sintetisani materijal je biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs). AgNPs su dobijene in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Korišćene su dve koncentracije CHI (0,1 mas.% and 0,5 mas.%), u cilju ispitivanja uticaja sadržaja CHI na svojstva nankompozitnih hidrogelova. Nanokompoziti Ag/PVA/CHI/Gr su dalje karakterisani metodom skenirajuće elektronske mikroskopije (SEM), Raman spektroskopije i rendgenske fotoelektronske spektroskopije (XPS). Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema Staphylococcus aureus i Escherichia coli je potvrđena agar-difuzionim testom, dok je citotoksičnost ispitana direktnim testom isključenja sa bojom tripan-plavo (DET).
AB  - Electrochemical methods were employed for synthesis of various composites intended for applications in medicine as antibacterial coatings on titanium bone implants or as highly efficient antimicrobial hydrogels for accelerated wound healing.
The first synthesized material is a composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS) which includes an antibiotic gentamicin, obtained by the method of electrophoretic deposition (EPD). EPD was carried out at the constant voltage on pure titanium plates from an aqueous suspension. Thus assembled porous homogenous coating would gain improved mechanical strength and favorable antibacterial properties. HAP/CS/Gent coating was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). Obtained results confirmed the formation of a new composite coating HAP/CS/Gent. Cytotoxicity against two types of cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The antibiotic loaded coating (HAP/CS/Gent) exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating the high potential for biomedical applications.
The second synthesized material is biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs). AgNPs were produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Two concentrations of CHI (0.1 wt% and 0.5 wt%) were used in order to investigate the influence of CHI content on the properties of nanocomposite hydrogels. The Ag/PVA/CHI/Gr nanocomposites were further characterized by scanning electron microscopy (SEM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against Staphylococcus aureus and Escherichia coli was confirmed by disc-diffusion tests, while low cytotoxicity was confirmed using trypan blue dye-exclusion test (DET).
PB  - Beograd : UDRUŽENJE MIKROBIOLOGA SRBIJE
C3  - XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO
T1  - Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene
EP  - 163
SP  - 162
ER  - 

@conference{
author = "Janković, Ana and Nešović, Katarina and Stevanović, Milena and Đošić, Marija and Kojić, Vesna and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Elektrohemijske metode su koris ćene za sintezu razlic itih kompozita namenjenih za aplikacije u medicini kao antibakterijske prevlake na koštanim titanskim implantatima ili kao visoko efikasni antimikrobni hidrogelovi za ubrzano zarastanje rana.
Prvi sintetisani materijal je kompozitna prevlaka hidroksiapatita (HAP) sa prirodnim polimerom hitozanom (CS) koja uključuje antibiotik gentamicin, dobijena metodom elektroforetskog taloz enja (EPD). EPD se izvodi pri konstantnom naponu na ploc icama c istog titana iz vodene suspenzije. Na ovaj nac in dobijena porozna homogena prevlaka ima poboljs anu mehanic ku c vrstoću i povoljne antibakterijske osobine. Prevlaka HAP/CS/Gent okarakterisana je difrakcijom X-zraka (XRD), infracrvenom spektroskopijom sa Furijeovom transformacijom (FT-IR) i skenirajućom elektronskom mikroskopijom (FE-SEM). Dobijeni rezultati potvrdili su formiranje nove kompozitne prevlake HAP/CS/Gent. Citotoksičnost prema dvema vrstama ćelijskih linija ispitana je koris ćenjem testa iskljuc ivanja tripan plave boje (DET) i MTT testa za procenu ćelijske metaboličke aktivnosti. Prevlaka sa antibiotikom (HAP/CS/Gent) pokazala je dobru antibakterijsku aktivnost protiv Staphylococcus aureus i Escherichia coli, dok je istovremeno zadržala nisku citotoksičnost, što ukazuje na visok potencijal za biomedicinske primene.
Drugi sintetisani materijal je biokompatibilni nanokompozitni hidrogel polivinil-alkohol/hitozan/grafen (PVA/CHI/Gr) sa nanočesticama srebra (AgNPs). AgNPs su dobijene in situ elektrohemijskom sintezom u matrici nabubrelih hidrogelova PVA/CHI/Gr. Korišćene su dve koncentracije CHI (0,1 mas.% and 0,5 mas.%), u cilju ispitivanja uticaja sadržaja CHI na svojstva nankompozitnih hidrogelova. Nanokompoziti Ag/PVA/CHI/Gr su dalje karakterisani metodom skenirajuće elektronske mikroskopije (SEM), Raman spektroskopije i rendgenske fotoelektronske spektroskopije (XPS). Otpuštanje srebra je praćeno tokom 28 dana, a dobijeni profili ukazuju na postepeno kontrolisano otpuštanje tokom ovog perioda. Antibakterijska aktivnost prema Staphylococcus aureus i Escherichia coli je potvrđena agar-difuzionim testom, dok je citotoksičnost ispitana direktnim testom isključenja sa bojom tripan-plavo (DET)., Electrochemical methods were employed for synthesis of various composites intended for applications in medicine as antibacterial coatings on titanium bone implants or as highly efficient antimicrobial hydrogels for accelerated wound healing.
The first synthesized material is a composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS) which includes an antibiotic gentamicin, obtained by the method of electrophoretic deposition (EPD). EPD was carried out at the constant voltage on pure titanium plates from an aqueous suspension. Thus assembled porous homogenous coating would gain improved mechanical strength and favorable antibacterial properties. HAP/CS/Gent coating was characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). Obtained results confirmed the formation of a new composite coating HAP/CS/Gent. Cytotoxicity against two types of cell lines was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The antibiotic loaded coating (HAP/CS/Gent) exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating the high potential for biomedical applications.
The second synthesized material is biocompatible poly(vinyl alcohol)/chitosan/graphene (PVA/CHI/Gr) nanocomposite hydrogel loaded with silver nanoparticles (AgNPs). AgNPs were produced by in situ electrochemical synthesis in the pre-swollen PVA/CHI/Gr hydrogel matrix. Two concentrations of CHI (0.1 wt% and 0.5 wt%) were used in order to investigate the influence of CHI content on the properties of nanocomposite hydrogels. The Ag/PVA/CHI/Gr nanocomposites were further characterized by scanning electron microscopy (SEM), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). Silver release was monitored for 28 days and the obtained profiles indicate slow controlled release over chosen time period. Antibacterial activity against Staphylococcus aureus and Escherichia coli was confirmed by disc-diffusion tests, while low cytotoxicity was confirmed using trypan blue dye-exclusion test (DET).",
publisher = "Beograd : UDRUŽENJE MIKROBIOLOGA SRBIJE",
journal = "XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO",
title = "Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene",
pages = "163-162"
}

Janković, A., Nešović, K., Stevanović, M., Đošić, M., Kojić, V., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2018). Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO
Beograd : UDRUŽENJE MIKROBIOLOGA SRBIJE., 162-163.

Janković A, Nešović K, Stevanović M, Đošić M, Kojić V, Vukašinović-Sekulić M, Mišković-Stanković V. Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene. in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO. 2018;:162-163..

Janković, Ana, Nešović, Katarina, Stevanović, Milena, Đošić, Marija, Kojić, Vesna, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Elektrohemijski sintetizovani bioaktivni kompoziti za medicinske primene" in XII Kongres mikrobiologa Srbije sa međunarodnim učešćem, Mikromed 2018 REGIO (2018):162-163.

TY  - CONF
AU  - Đošić, Marija
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/765
AB  - Elektroforetsko taloženje je efikasna elektrohemijska metoda za dobijanje višekomponentnih
biokeramičkih prevlaka na metalnom supstratu. Za formiranje biomaterijala za
koštane implantate, najbolji rezultati su dobijeni kod prevlaka koje predstavljaju kombinaciju
mineralne faze i polimera. U cilju dobijanja bioaktivnih prevlaka na titanu primenom
elektroforetskog taloženja, urađena je optimizacija uslova taloženja iz trokomponentne
vodene suspenzije hidroksiapatita (HAP), hitozana (CS) i antibiotika gentamicina (Gent), pri
različitim konstantnim vrednostima napona i vremena taloženja. Karakterizacija prevlaka je
izvršena primenom rendgenske difrakcione analize (XRD) i rendgenske fotoelektronske
spektroskopije (XPS). Citotoksičnost dobijenih prevlaka je ispitana MTT testom, dok je
antibakterijska aktivnost prevlake sa gentamicinom potvrđena primenom testa u suspenziji.
Na osnovu dobijenih rezultata, potvrđeno je da trokomponentna biokompatibilna prevlaka
HAP/CS/Gent ima veliki potencijal za upotrebu u biomedicini.
AB  - Electrophoretic deposition is a suitable method for obtaining multicomponent bioceramic
coatings on titanium (Ti). For hard tissue implants, the best results are achieved for the
coating composed of mineral phase and a polymer. First step in producing bioactive
coatings on Ti using electrophoretic deposition process was the optimization of deposition
parameters for three-component aqueous suspension, containing hydroxyapatite (HAP),
chitosan (CS) and gentamicin (Gent), at constant values of applied voltages and different
deposition times. The coatings obtained were characterized by X-ray diffraction (XRD) and
X-ray photoelectron spectroscopy (XPS). Cytotoxicity of the coatings was evaluated by MTT
assay, and the antibacterial activity of gentamicin loaded coating was verified using test in
suspension. Based on the experimental results, the formation of a new HAP/CS/Gent
composite coating with future prospect for biomedical use was confirmed.
PB  - Belgrade : Serbian Chemical Society
C3  - 55. savetovanje Srpskog hemijskog društva
T1  - Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu
EP  - 22
SP  - 22
ER  - 

@conference{
author = "Đošić, Marija and Stevanović, Milena and Janković, Ana and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Elektroforetsko taloženje je efikasna elektrohemijska metoda za dobijanje višekomponentnih
biokeramičkih prevlaka na metalnom supstratu. Za formiranje biomaterijala za
koštane implantate, najbolji rezultati su dobijeni kod prevlaka koje predstavljaju kombinaciju
mineralne faze i polimera. U cilju dobijanja bioaktivnih prevlaka na titanu primenom
elektroforetskog taloženja, urađena je optimizacija uslova taloženja iz trokomponentne
vodene suspenzije hidroksiapatita (HAP), hitozana (CS) i antibiotika gentamicina (Gent), pri
različitim konstantnim vrednostima napona i vremena taloženja. Karakterizacija prevlaka je
izvršena primenom rendgenske difrakcione analize (XRD) i rendgenske fotoelektronske
spektroskopije (XPS). Citotoksičnost dobijenih prevlaka je ispitana MTT testom, dok je
antibakterijska aktivnost prevlake sa gentamicinom potvrđena primenom testa u suspenziji.
Na osnovu dobijenih rezultata, potvrđeno je da trokomponentna biokompatibilna prevlaka
HAP/CS/Gent ima veliki potencijal za upotrebu u biomedicini., Electrophoretic deposition is a suitable method for obtaining multicomponent bioceramic
coatings on titanium (Ti). For hard tissue implants, the best results are achieved for the
coating composed of mineral phase and a polymer. First step in producing bioactive
coatings on Ti using electrophoretic deposition process was the optimization of deposition
parameters for three-component aqueous suspension, containing hydroxyapatite (HAP),
chitosan (CS) and gentamicin (Gent), at constant values of applied voltages and different
deposition times. The coatings obtained were characterized by X-ray diffraction (XRD) and
X-ray photoelectron spectroscopy (XPS). Cytotoxicity of the coatings was evaluated by MTT
assay, and the antibacterial activity of gentamicin loaded coating was verified using test in
suspension. Based on the experimental results, the formation of a new HAP/CS/Gent
composite coating with future prospect for biomedical use was confirmed.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "55. savetovanje Srpskog hemijskog društva",
title = "Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu",
pages = "22-22"
}

Đošić, M., Stevanović, M., Janković, A., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2018). Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu. in 55. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 22-22.

Đošić M, Stevanović M, Janković A, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu. in 55. savetovanje Srpskog hemijskog društva. 2018;:22-22..

Đošić, Marija, Stevanović, Milena, Janković, Ana, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu" in 55. savetovanje Srpskog hemijskog društva (2018):22-22.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/764
AB  - Sposobnost osteointegracije i izvanredna biokompatibilnost hidroksiapatita (HAP) u
kombinaciji sa dobrim mehaničkim i antimikrobnim svojstvima hitozana (CS) uz dodatak
antibiotika gentamicina (Gent) omogućava dobijanje biokompatibilnih prevlaka sa
poboljšanom biološkom funkcijom. U cilju povećanja biokompatibilnosti i bioaktivnosti
titanskih (Ti) implantata, izvršena je modifikacija površine Ti nanošenjem biokeramičke
HAP/CS/Gent prevlake postupkom elektroforetskog taloženja (EPD). Taloženje je izvedeno
u jednom stupnju, iz trokomponentne vodene suspenzije pri konstantnom naponu.
Karakterizacija dobijenih prevlaka je izvršena primenom infracrvene spektroskopije sa
Furijeovom transformacijom (FT-IR) i skenirajuće elektronske mikroskopije (FE-SEM).
Citotoksičnost dobijenih prevlaka je ispitana MTT testom, a primenom testa u suspenziji
verifikovana je antibakterijska aktivnost. Dobijeni rezultati su potvrdili formiranje nove
kompozitne prevlake HAP/CS/Gent sa velikim potencijalom za upotrebu u biomedicini.
AB  - The ability of osseointegration and excellent biocompatibility of hydroxyapatite (HAP)
combined with good mechanical and antimicrobial properties of chitosan (CS) with the
addition of antibiotic gentamicin (Gent), enabled the production of biocompatible coatings
with improved biological function. In order to increase the biocompatibility and bioactivity
of titanium (Ti) implants, Ti surface was modified by applying the bioceramic HAP/CS/Gent
coating using electrophoretic deposition process (EPD). Deposition was carried out in one
step from a three-component aqueous suspension (HAP/CS/Gent) at constant voltage. The
obtained coatings were characterized by Fourier transform infrared spectroscopy (FT-IR),
and field emission scanning electron microscopy (FE-SEM). The cytotoxicity of the obtained
coatings was evaulated by MTT assay, and the antibacterial activity was verified using test
in suspension. Results obtained confirmed the formation of a new HAP/CS/Gent composite
coating with future prospect for biomedical use.
PB  - Belgrade : Serbian Chemical Society
C3  - 55. savetovanje Srpskog hemijskog društva
T1  - Elektroforetski taložene biokeramičke kompozitne prevlake sa gentamicinom za biomedicinsku namenu u formi koštanih implantata
EP  - 21
SP  - 21
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Sposobnost osteointegracije i izvanredna biokompatibilnost hidroksiapatita (HAP) u
kombinaciji sa dobrim mehaničkim i antimikrobnim svojstvima hitozana (CS) uz dodatak
antibiotika gentamicina (Gent) omogućava dobijanje biokompatibilnih prevlaka sa
poboljšanom biološkom funkcijom. U cilju povećanja biokompatibilnosti i bioaktivnosti
titanskih (Ti) implantata, izvršena je modifikacija površine Ti nanošenjem biokeramičke
HAP/CS/Gent prevlake postupkom elektroforetskog taloženja (EPD). Taloženje je izvedeno
u jednom stupnju, iz trokomponentne vodene suspenzije pri konstantnom naponu.
Karakterizacija dobijenih prevlaka je izvršena primenom infracrvene spektroskopije sa
Furijeovom transformacijom (FT-IR) i skenirajuće elektronske mikroskopije (FE-SEM).
Citotoksičnost dobijenih prevlaka je ispitana MTT testom, a primenom testa u suspenziji
verifikovana je antibakterijska aktivnost. Dobijeni rezultati su potvrdili formiranje nove
kompozitne prevlake HAP/CS/Gent sa velikim potencijalom za upotrebu u biomedicini., The ability of osseointegration and excellent biocompatibility of hydroxyapatite (HAP)
combined with good mechanical and antimicrobial properties of chitosan (CS) with the
addition of antibiotic gentamicin (Gent), enabled the production of biocompatible coatings
with improved biological function. In order to increase the biocompatibility and bioactivity
of titanium (Ti) implants, Ti surface was modified by applying the bioceramic HAP/CS/Gent
coating using electrophoretic deposition process (EPD). Deposition was carried out in one
step from a three-component aqueous suspension (HAP/CS/Gent) at constant voltage. The
obtained coatings were characterized by Fourier transform infrared spectroscopy (FT-IR),
and field emission scanning electron microscopy (FE-SEM). The cytotoxicity of the obtained
coatings was evaulated by MTT assay, and the antibacterial activity was verified using test
in suspension. Results obtained confirmed the formation of a new HAP/CS/Gent composite
coating with future prospect for biomedical use.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "55. savetovanje Srpskog hemijskog društva",
title = "Elektroforetski taložene biokeramičke kompozitne prevlake sa gentamicinom za biomedicinsku namenu u formi koštanih implantata",
pages = "21-21"
}

Stevanović, M., Đošić, M., Janković, A., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2018). Elektroforetski taložene biokeramičke kompozitne prevlake sa gentamicinom za biomedicinsku namenu u formi koštanih implantata. in 55. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 21-21.

Stevanović M, Đošić M, Janković A, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Elektroforetski taložene biokeramičke kompozitne prevlake sa gentamicinom za biomedicinsku namenu u formi koštanih implantata. in 55. savetovanje Srpskog hemijskog društva. 2018;:21-21..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Elektroforetski taložene biokeramičke kompozitne prevlake sa gentamicinom za biomedicinsku namenu u formi koštanih implantata" in 55. savetovanje Srpskog hemijskog društva (2018):21-21.

TY  - CONF
AU  - Stevanović, Milena
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Kojić, Vesna
AU  - Mišković-Stanković, Vesna
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/770
AB  - A single-step electrophoretic deposition (EPD) process from aqueous suspension was used to
assess novel composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS)
and antibiotic, gentamicin (Gent). EPD was carried out at constant voltage on pure titanium
plates. In order to investigate the gentamicin influence on physico-chemical and biological
properties of composite coatings, detailed characterization was performed. For that purpose
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and fieldemission
scanning electron microscopy (FE-SEM) were used. Obtained results confirmed the
formation of a new composite HAP/CS/Gent coating. Potential application was examined
through biological assays. The gentamicin addition strongly improved the antibacterial
activity of HAP/CS/Gent coating that was confirmed by agar diffusion method and test in
suspension. Obtained results indicated more pronounced antibacterial effect against
Staphylococcus aureus compared to Escherichia coli. MTT and DET tests indicated low
cytotoxicity against MRC-5 and L929 tested cell lines, indicating good biocompatibility of
obtained composites. MRC-5 human cell line responded better in cytotoxicity testing,
pointing to encouraging results for potential application. The ability of biomineralization
promotion was noticed through the ALP assay. Therefore, HAP/CS/Gent coating can be
considered as perspective material for bone tissue engineering as hard tissue implant.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Seventeenth Young Researchers Conference – Materials Science and Engineering
T1  - Antibiotic loaded bioactive orthopedic implant coating
EP  - 2
SP  - 2
ER  - 

@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2018",
abstract = "A single-step electrophoretic deposition (EPD) process from aqueous suspension was used to
assess novel composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS)
and antibiotic, gentamicin (Gent). EPD was carried out at constant voltage on pure titanium
plates. In order to investigate the gentamicin influence on physico-chemical and biological
properties of composite coatings, detailed characterization was performed. For that purpose
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and fieldemission
scanning electron microscopy (FE-SEM) were used. Obtained results confirmed the
formation of a new composite HAP/CS/Gent coating. Potential application was examined
through biological assays. The gentamicin addition strongly improved the antibacterial
activity of HAP/CS/Gent coating that was confirmed by agar diffusion method and test in
suspension. Obtained results indicated more pronounced antibacterial effect against
Staphylococcus aureus compared to Escherichia coli. MTT and DET tests indicated low
cytotoxicity against MRC-5 and L929 tested cell lines, indicating good biocompatibility of
obtained composites. MRC-5 human cell line responded better in cytotoxicity testing,
pointing to encouraging results for potential application. The ability of biomineralization
promotion was noticed through the ALP assay. Therefore, HAP/CS/Gent coating can be
considered as perspective material for bone tissue engineering as hard tissue implant.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Seventeenth Young Researchers Conference – Materials Science and Engineering",
title = "Antibiotic loaded bioactive orthopedic implant coating",
pages = "2-2"
}

Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2018). Antibiotic loaded bioactive orthopedic implant coating. in Seventeenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 2-2.

Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Antibiotic loaded bioactive orthopedic implant coating. in Seventeenth Young Researchers Conference – Materials Science and Engineering. 2018;:2-2..

Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Antibiotic loaded bioactive orthopedic implant coating" in Seventeenth Young Researchers Conference – Materials Science and Engineering (2018):2-2.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2017
UR  - https://ritnms.itnms.ac.rs/handle/123456789/760
AB  - A broad spectrum antibiotic-gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymer chitosan (CS) using electrophoretic deposition (EPD) technique.
EPD was carried out at the constant voltage on pure titanium plates from an aqueous
suspension. The aim was to assemble porous homogenous coating with improved mechanical
strength and favourable antibacterial properties. HAP/CS/Gent coating was characterized by
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR),
thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM).
Obtained results confirmed the formation of a new composite coating HAP/CS/Gent.
In the XRD spectrum of HAP/CS/Gent only characteristic crystal planes of HAP were
detected. FTIR analysis confirmed the formation of intermolecular and intramolecular
hydrogen bonds in the composite coating. TGA analysis was employed to investigate the
thermal stability of HAP/CS/Gent. The bioactivity of HAP/CS/Gent composite coating was
proved by SEM analysis. The obtained SEM micrographs indicate the biomimetic growth of
a new apatite layer after immersion in SBF. The antibiotic loaded coating (HAP/CS/Gent)
exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli,
indicating the high potential for biomedical applications. Additionally, the results have
shown that the antibacterial effect of HAP/CS/Gent was more pronounced against S. aureus
compared to E. coli.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Sixteenth Young Researchers Conference – Materials Science and Engineering
T1  - Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin
EP  - 14
SP  - 14
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2017",
abstract = "A broad spectrum antibiotic-gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymer chitosan (CS) using electrophoretic deposition (EPD) technique.
EPD was carried out at the constant voltage on pure titanium plates from an aqueous
suspension. The aim was to assemble porous homogenous coating with improved mechanical
strength and favourable antibacterial properties. HAP/CS/Gent coating was characterized by
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR),
thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM).
Obtained results confirmed the formation of a new composite coating HAP/CS/Gent.
In the XRD spectrum of HAP/CS/Gent only characteristic crystal planes of HAP were
detected. FTIR analysis confirmed the formation of intermolecular and intramolecular
hydrogen bonds in the composite coating. TGA analysis was employed to investigate the
thermal stability of HAP/CS/Gent. The bioactivity of HAP/CS/Gent composite coating was
proved by SEM analysis. The obtained SEM micrographs indicate the biomimetic growth of
a new apatite layer after immersion in SBF. The antibiotic loaded coating (HAP/CS/Gent)
exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli,
indicating the high potential for biomedical applications. Additionally, the results have
shown that the antibacterial effect of HAP/CS/Gent was more pronounced against S. aureus
compared to E. coli.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Sixteenth Young Researchers Conference – Materials Science and Engineering",
title = "Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin",
pages = "14-14"
}

Stevanović, M., Đošić, M., Janković, A., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2017). Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin. in Sixteenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 14-14.

Stevanović M, Đošić M, Janković A, Vukašinović-Sekulić M, Mišković-Stanković V. Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin. in Sixteenth Young Researchers Conference – Materials Science and Engineering. 2017;:14-14..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Electrochemically obtained bioactive nanocomposite coating based on hydroxyapatite and chitosan loaded with gentamicin" in Sixteenth Young Researchers Conference – Materials Science and Engineering (2017):14-14.

TY  - CONF
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2017
UR  - https://ritnms.itnms.ac.rs/handle/123456789/762
AB  - Electrophoretic deposition is an effective method for obtaining uniform coatings of precisely
controlled thickness and morphology. In this research, a single step electophoretic deposition
process for obtaining nanocomposite coating on titanium from a three-component aqueous
hydroxyapatite/chitosan/ gentamicin (HAP/CHI/Gent) suspension was investigated. The deposition
was carried out at a constant voltage, after the metal surface pretreatment. Characterization of the
coating was performed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),
field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy
(XPS). Obtained results confirm the formation of a new composite coating HAP/CHI/Gent which
exhibits strong antibacterial activity and has the potential to be used as an orthopedic and dental
bone tissue implant or as a drug carrier.
AB  - Elektroforetsko taloženje predstavlja efikasan postupak za dobijanje uniformnih keramičkih biokompatibilnih prevlaka kontrolisane debljine i morfologije. U ovom radu je ispitano elektroforetsko taloženje u jednom stupnju kompozitnih prevlaka na titanu iz trokomponentne vodene suspenzije hidroksiapatit/hitozan/gentamicin (HAP/CHI/Gent). Taloženje je izvedeno pri konstatnom naponu i uz prethodnu pripremu površine metala. Karakterizacija prevlaka je izvršena rendgenskom difrakcionom analizom (XRD), infracrvenom spektroskopijom sa Furijeovom transformacijom (FTIR), skenirajućom emisionom mikroskopijom (FE-SEM) i spektroskopijom fotoelektrona izazvanih X-zračenjem (XPS). Dobijeni rezultati potvrđuju formiranje nove kompozitne prevlake HAP/CHI/Gent koja pokazuje jaku antibakterijsku aktivnost i ima potencijal da se koristi kao ortopedski i dentalni implantat koštanog tkiva ili kao nosač lekova.
C3  - 54. SAVETOVANJE SRPSKOG HEMIJSKOG DRUŠTVA I 5. KONFERENCIJA MLADIH HEMIČARA SRBIJA
T1  - Biokompatibilna prevlaka hidroksiapatit/hitozan/gentamicin dobijena postupkom elektroforetskog taloženja na titanu iz vodene suspenzije
EP  - 26
SP  - 26
ER  - 

@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2017",
abstract = "Electrophoretic deposition is an effective method for obtaining uniform coatings of precisely
controlled thickness and morphology. In this research, a single step electophoretic deposition
process for obtaining nanocomposite coating on titanium from a three-component aqueous
hydroxyapatite/chitosan/ gentamicin (HAP/CHI/Gent) suspension was investigated. The deposition
was carried out at a constant voltage, after the metal surface pretreatment. Characterization of the
coating was performed by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),
field emission scanning electron microscopy (FE-SEM) and X-ray photoelectron spectroscopy
(XPS). Obtained results confirm the formation of a new composite coating HAP/CHI/Gent which
exhibits strong antibacterial activity and has the potential to be used as an orthopedic and dental
bone tissue implant or as a drug carrier., Elektroforetsko taloženje predstavlja efikasan postupak za dobijanje uniformnih keramičkih biokompatibilnih prevlaka kontrolisane debljine i morfologije. U ovom radu je ispitano elektroforetsko taloženje u jednom stupnju kompozitnih prevlaka na titanu iz trokomponentne vodene suspenzije hidroksiapatit/hitozan/gentamicin (HAP/CHI/Gent). Taloženje je izvedeno pri konstatnom naponu i uz prethodnu pripremu površine metala. Karakterizacija prevlaka je izvršena rendgenskom difrakcionom analizom (XRD), infracrvenom spektroskopijom sa Furijeovom transformacijom (FTIR), skenirajućom emisionom mikroskopijom (FE-SEM) i spektroskopijom fotoelektrona izazvanih X-zračenjem (XPS). Dobijeni rezultati potvrđuju formiranje nove kompozitne prevlake HAP/CHI/Gent koja pokazuje jaku antibakterijsku aktivnost i ima potencijal da se koristi kao ortopedski i dentalni implantat koštanog tkiva ili kao nosač lekova.",
journal = "54. SAVETOVANJE SRPSKOG HEMIJSKOG DRUŠTVA I 5. KONFERENCIJA MLADIH HEMIČARA SRBIJA",
title = "Biokompatibilna prevlaka hidroksiapatit/hitozan/gentamicin dobijena postupkom elektroforetskog taloženja na titanu iz vodene suspenzije",
pages = "26-26"
}

Stevanović, M., Đošić, M., Janković, A., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2017). Biokompatibilna prevlaka hidroksiapatit/hitozan/gentamicin dobijena postupkom elektroforetskog taloženja na titanu iz vodene suspenzije. in 54. SAVETOVANJE SRPSKOG HEMIJSKOG DRUŠTVA I 5. KONFERENCIJA MLADIH HEMIČARA SRBIJA, 26-26.

Stevanović M, Đošić M, Janković A, Vukašinović-Sekulić M, Mišković-Stanković V. Biokompatibilna prevlaka hidroksiapatit/hitozan/gentamicin dobijena postupkom elektroforetskog taloženja na titanu iz vodene suspenzije. in 54. SAVETOVANJE SRPSKOG HEMIJSKOG DRUŠTVA I 5. KONFERENCIJA MLADIH HEMIČARA SRBIJA. 2017;:26-26..

Stevanović, Milena, Đošić, Marija, Janković, Ana, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Biokompatibilna prevlaka hidroksiapatit/hitozan/gentamicin dobijena postupkom elektroforetskog taloženja na titanu iz vodene suspenzije" in 54. SAVETOVANJE SRPSKOG HEMIJSKOG DRUŠTVA I 5. KONFERENCIJA MLADIH HEMIČARA SRBIJA (2017):26-26.

TY  - CONF
AU  - Ilić, Iva
AU  - Janković, Ana
AU  - Đošić, Marija
AU  - Vukašinović-Sekulić, Maja
AU  - Mišković-Stanković, Vesna
PY  - 2017
UR  - https://ritnms.itnms.ac.rs/handle/123456789/761
AB  - Biocomposite ceramic coatings hydroxyapatite (HAP), silver/hydroxyapatite (Ag/HAP) and
silver/hydroxyapatite/graphene (Ag/HAP/Gr) are obtained on titanium by electrophoretic
deposition (EPD). The goal of this research was to develop multifunctional composite
coatings as a suitable strategy to improve the surface properties of metallic implants.
The deposition was performed on titanium electrodes with specific geometry suitable for
implantation in the frontal bone of rabbits for in vivo experiments. The effect of deposition
parameters on deposited mass was investigated by varying different values of constant
voltage for various deposition time. Uniform and compact HAP coatings were successfully
deposited at applied voltage of 30 V for 45 s, whereas Ag/HAP and Ag/HAP/Gr coatings
with satisfying adhesion and coverage were deposited at applied voltage of 60 V for 1 min.
Bearing in mind certain toxic effects of the nanoparticle silver, the deposition of the coating
of hydroxyapatite/chitosan/graphene (HAP/CS/Gr) of the same composition with the
addition of the antibiotic gentamicin was performed, in order to replace the silver
nanoparticles. The antimicrobial activity of the coating HAP/CS/Gr without and with
gentamicin against Staphylococcus aureus and Escherichia coli was examined by agar
diffusion test. Moreover, disc diffusion test showed improved bactericidal effect of
gentamicin-loaded composite coatings against Staphylococcus aureus and Escherichia coli
compared to control non-gentamicin-loaded coatings.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Sixteenth Young Researchers Conference – Materials Science and Engineering
T1  - Electrophoretic deposition of biocomposite ceramic coatings of hydroxyapatite and chitosan with silver and gentamicin on titanium for medical applications
EP  - 4
SP  - 4
ER  - 

@conference{
author = "Ilić, Iva and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna",
year = "2017",
abstract = "Biocomposite ceramic coatings hydroxyapatite (HAP), silver/hydroxyapatite (Ag/HAP) and
silver/hydroxyapatite/graphene (Ag/HAP/Gr) are obtained on titanium by electrophoretic
deposition (EPD). The goal of this research was to develop multifunctional composite
coatings as a suitable strategy to improve the surface properties of metallic implants.
The deposition was performed on titanium electrodes with specific geometry suitable for
implantation in the frontal bone of rabbits for in vivo experiments. The effect of deposition
parameters on deposited mass was investigated by varying different values of constant
voltage for various deposition time. Uniform and compact HAP coatings were successfully
deposited at applied voltage of 30 V for 45 s, whereas Ag/HAP and Ag/HAP/Gr coatings
with satisfying adhesion and coverage were deposited at applied voltage of 60 V for 1 min.
Bearing in mind certain toxic effects of the nanoparticle silver, the deposition of the coating
of hydroxyapatite/chitosan/graphene (HAP/CS/Gr) of the same composition with the
addition of the antibiotic gentamicin was performed, in order to replace the silver
nanoparticles. The antimicrobial activity of the coating HAP/CS/Gr without and with
gentamicin against Staphylococcus aureus and Escherichia coli was examined by agar
diffusion test. Moreover, disc diffusion test showed improved bactericidal effect of
gentamicin-loaded composite coatings against Staphylococcus aureus and Escherichia coli
compared to control non-gentamicin-loaded coatings.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Sixteenth Young Researchers Conference – Materials Science and Engineering",
title = "Electrophoretic deposition of biocomposite ceramic coatings of hydroxyapatite and chitosan with silver and gentamicin on titanium for medical applications",
pages = "4-4"
}

Ilić, I., Janković, A., Đošić, M., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2017). Electrophoretic deposition of biocomposite ceramic coatings of hydroxyapatite and chitosan with silver and gentamicin on titanium for medical applications. in Sixteenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 4-4.

Ilić I, Janković A, Đošić M, Vukašinović-Sekulić M, Mišković-Stanković V. Electrophoretic deposition of biocomposite ceramic coatings of hydroxyapatite and chitosan with silver and gentamicin on titanium for medical applications. in Sixteenth Young Researchers Conference – Materials Science and Engineering. 2017;:4-4..

Ilić, Iva, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Electrophoretic deposition of biocomposite ceramic coatings of hydroxyapatite and chitosan with silver and gentamicin on titanium for medical applications" in Sixteenth Young Researchers Conference – Materials Science and Engineering (2017):4-4.