Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium
Autori
Stevanović, MilenaJanković, Ana
Đošić, Marija
Vukašinović-Sekulić, Maja
Kojić, Vesna
Mišković-Stanković, Vesna
Ostala autorstva
Marković, SmiljaKonferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity a...gainst two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants.
Izvor:
Twentieth Young Researchers Conference – Materials Science and Engineering, 2022, 15-15Izdavač:
- Belgrade : Institute of Technical Sciences of SASA
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200023 (Institut za tehnologiju nuklearnih i drugih mineralnih sirovina - ITNMS, Beograd) (RS-MESTD-inst-2020-200023)
Institucija/grupa
Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - CONF AU - Stevanović, Milena AU - Janković, Ana AU - Đošić, Marija AU - Vukašinović-Sekulić, Maja AU - Kojić, Vesna AU - Mišković-Stanković, Vesna PY - 2022 UR - https://ritnms.itnms.ac.rs/handle/123456789/752 AB - Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion resistance (naturally forms a passive oxide layer) and good mechanical properties. Although Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be significantly improved by modifying its surface. In order to improve antibacterial properties a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite (HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). The obtained results confirmed the formation of new composite coatings. Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating the high potential for biomedical applications. Excellent osteogenic properties, through the promotion of osteoblast differentiation were confirmed by ALP assay. Results were much more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating can be considered as an excellent promising candidate for biomedical hard tissue implants. PB - Belgrade : Institute of Technical Sciences of SASA C3 - Twentieth Young Researchers Conference – Materials Science and Engineering T1 - Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium EP - 15 SP - 15 ER -
@conference{ author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna", year = "2022", abstract = "Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion resistance (naturally forms a passive oxide layer) and good mechanical properties. Although Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be significantly improved by modifying its surface. In order to improve antibacterial properties a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite (HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy (FE-SEM). The obtained results confirmed the formation of new composite coatings. Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating the high potential for biomedical applications. Excellent osteogenic properties, through the promotion of osteoblast differentiation were confirmed by ALP assay. Results were much more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating can be considered as an excellent promising candidate for biomedical hard tissue implants.", publisher = "Belgrade : Institute of Technical Sciences of SASA", journal = "Twentieth Young Researchers Conference – Materials Science and Engineering", title = "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium", pages = "15-15" }
Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2022). Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering Belgrade : Institute of Technical Sciences of SASA., 15-15.
Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium. in Twentieth Young Researchers Conference – Materials Science and Engineering. 2022;:15-15..
Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium" in Twentieth Young Researchers Conference – Materials Science and Engineering (2022):15-15.