dc.contributor | Marković, Smilja | |
dc.creator | Stevanović, Milena | |
dc.creator | Janković, Ana | |
dc.creator | Đošić, Marija | |
dc.creator | Vukašinović-Sekulić, Maja | |
dc.creator | Kojić, Vesna | |
dc.creator | Mišković-Stanković, Vesna | |
dc.date.accessioned | 2023-09-21T08:39:43Z | |
dc.date.available | 2023-09-21T08:39:43Z | |
dc.date.issued | 2022 | |
dc.identifier.isbn | 978-86-80321-37-0 | |
dc.identifier.uri | https://ritnms.itnms.ac.rs/handle/123456789/752 | |
dc.description.abstract | Titanium (Ti) is an implant material widely used in orthopedics due to its high corrosion
resistance (naturally forms a passive oxide layer) and good mechanical properties. Although
Ti has certain ability to osseointegrate, the biocompatibility and bioactivity of Ti can be
significantly improved by modifying its surface. In order to improve antibacterial properties
a broad spectrum antibiotic gentamicin was loaded in a composite coating of hydroxyapatite
(HAP) with natural polymers chitosan (CS) and poly(vinyl alcohol (PVA) and was deposited
on Ti using electrophoretic deposition (EPD) technique. EPD was carried out at the constant
voltage on pure titanium plates from an aqueous suspension. Deposited HAP/CS/PVA and
HAP/CS/PVA/Gent coating were characterized by X-ray diffraction (XRD), Fourier
transform infrared spectroscopy (FT-IR), and field emission scanning electron microscopy
(FE-SEM). The obtained results confirmed the formation of new composite coatings.
Cytotoxicity against two types of cell lines (MRC-5 and L929) was investigated using trypan
blue dye-exclusion test (DET) and MTT assay for assessing cell metabolic activity. The
antibiotic loaded coating (HAP/CS/PVA/Gent) exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, while preserving low cytotoxicity, indicating
the high potential for biomedical applications. Excellent osteogenic properties, through the
promotion of osteoblast differentiation were confirmed by ALP assay. Results were much
more pronounced for HAP/CS/PVA/Gent coating. Therefore, HAP/CS/PVA/Gent coating
can be considered as an excellent promising candidate for biomedical hard tissue implants. | sr |
dc.language.iso | en | sr |
dc.publisher | Belgrade : Institute of Technical Sciences of SASA | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200135/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200287/RS// | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200023/RS// | sr |
dc.rights | openAccess | sr |
dc.source | Twentieth Young Researchers Conference – Materials Science and Engineering | sr |
dc.title | Bioactive hydroxyapatite/chitosan/poly(vinyl alcohol)/gentamicin composite coating electrodeposited on titanium | sr |
dc.type | conferenceObject | sr |
dc.rights.license | ARR | sr |
dc.citation.epage | 15 | |
dc.citation.spage | 15 | |
dc.identifier.fulltext | http://ritnms.itnms.ac.rs/bitstream/id/1241/bitstream_1241.pdf | |
dc.type.version | publishedVersion | sr |