Luminescent fluoroapatite nano-biomaterial for labeling yeast cells as an innovative approach for identification, imaging and monitoring
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INTRODUCTION: The ubiquity of pathogenic yeast species in the human body and the increasing number of immunocompromised people acquiring infections have drawn attention to fungal infections [1]. Improved diagnostic imaging techniques and tools to study infection are necessary due to the commensal nature of pathogens yeast and the severity of the diseases they cause. The ability to label non-pathogenic yeast cells, such as the budding yeast Saccharomyces cerevisiae (S. cerevisiae), may facilitate the identification and monitoring of these microbes in different environments. The topic of interest in this research is the development of luminescent nano-biomaterials based on fluorapatite as a contrast agent for labeling and imaging S. cerevisiae. EXPERIMENTAL (or Materials and Methods): The method used to manufacture fluorapatite nanopowder has been previously reported in research [2]. After being acquired locally, S. cerevisiae was suspended in ...saline. One milligram of the FAP sample was added to the yeast suspension. After mixing the resultant suspension, it was left to incubate at room temperature for one hour without being stirred. Following treatment, cells were taken out, preserved, and prepared in triplicate for microscopy. MIPAR software has been used to analysethe obtained images. RESULTS AND DISCUSSION: Luminescent FAP nanoparticles were synthesized by precipitation and centrifugation at low temperature. The resulting single-phase nanomaterial exhibits cascade fluorescence in the violet and blue regions [2]. To investigate the performance of FAP nanoparticle fluorophores, cells of S. cerevisiae were labeled and observed with a Leica DMIL inverted fluorescence microscope. Nanofluoroapatite fluorophores were successfully labeled S. cerevisiae cells.MIPAR image analysis software extracted the luminescence of nano-biomaterials from yeast cells. CONCLUSIONS: In this study, S. cerevisiae was used as a yeast model which, after labelling with a fluorapatite-based contrast agent, showed luminescent properties. The cascading nature of the agents' luminescence will allow us to monitor cellular uptake as well as monitoring cellular localization in future studies.
Keywords:
bioimaging / nano-apatite / contrast agent / Saccharomyces cerevisiae / cascade luminescence / monitoringSource:
ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, HEMIJSKA INDUSTRIJA (Chemical Industry), 2024, 78, 1S, 67-67Publisher:
- Beograd : Savez hemijskih inženjera Srbije
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200023 (Institute of Technology of Nuclear and Other Mineral Row Materials - ITNMS, Belgrade) (RS-MESTD-inst-2020-200023)
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Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - JOUR AU - Milojkov, Dušan AU - Jovanović, Gvozden AU - Živković-Radovanović, Vukosava PY - 2024 UR - https://ritnms.itnms.ac.rs/handle/123456789/1249 AB - INTRODUCTION: The ubiquity of pathogenic yeast species in the human body and the increasing number of immunocompromised people acquiring infections have drawn attention to fungal infections [1]. Improved diagnostic imaging techniques and tools to study infection are necessary due to the commensal nature of pathogens yeast and the severity of the diseases they cause. The ability to label non-pathogenic yeast cells, such as the budding yeast Saccharomyces cerevisiae (S. cerevisiae), may facilitate the identification and monitoring of these microbes in different environments. The topic of interest in this research is the development of luminescent nano-biomaterials based on fluorapatite as a contrast agent for labeling and imaging S. cerevisiae. EXPERIMENTAL (or Materials and Methods): The method used to manufacture fluorapatite nanopowder has been previously reported in research [2]. After being acquired locally, S. cerevisiae was suspended in saline. One milligram of the FAP sample was added to the yeast suspension. After mixing the resultant suspension, it was left to incubate at room temperature for one hour without being stirred. Following treatment, cells were taken out, preserved, and prepared in triplicate for microscopy. MIPAR software has been used to analysethe obtained images. RESULTS AND DISCUSSION: Luminescent FAP nanoparticles were synthesized by precipitation and centrifugation at low temperature. The resulting single-phase nanomaterial exhibits cascade fluorescence in the violet and blue regions [2]. To investigate the performance of FAP nanoparticle fluorophores, cells of S. cerevisiae were labeled and observed with a Leica DMIL inverted fluorescence microscope. Nanofluoroapatite fluorophores were successfully labeled S. cerevisiae cells.MIPAR image analysis software extracted the luminescence of nano-biomaterials from yeast cells. CONCLUSIONS: In this study, S. cerevisiae was used as a yeast model which, after labelling with a fluorapatite-based contrast agent, showed luminescent properties. The cascading nature of the agents' luminescence will allow us to monitor cellular uptake as well as monitoring cellular localization in future studies. PB - Beograd : Savez hemijskih inženjera Srbije T2 - ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, HEMIJSKA INDUSTRIJA (Chemical Industry) T1 - Luminescent fluoroapatite nano-biomaterial for labeling yeast cells as an innovative approach for identification, imaging and monitoring EP - 67 IS - 1S SP - 67 VL - 78 ER -
@article{ author = "Milojkov, Dušan and Jovanović, Gvozden and Živković-Radovanović, Vukosava", year = "2024", abstract = "INTRODUCTION: The ubiquity of pathogenic yeast species in the human body and the increasing number of immunocompromised people acquiring infections have drawn attention to fungal infections [1]. Improved diagnostic imaging techniques and tools to study infection are necessary due to the commensal nature of pathogens yeast and the severity of the diseases they cause. The ability to label non-pathogenic yeast cells, such as the budding yeast Saccharomyces cerevisiae (S. cerevisiae), may facilitate the identification and monitoring of these microbes in different environments. The topic of interest in this research is the development of luminescent nano-biomaterials based on fluorapatite as a contrast agent for labeling and imaging S. cerevisiae. EXPERIMENTAL (or Materials and Methods): The method used to manufacture fluorapatite nanopowder has been previously reported in research [2]. After being acquired locally, S. cerevisiae was suspended in saline. One milligram of the FAP sample was added to the yeast suspension. After mixing the resultant suspension, it was left to incubate at room temperature for one hour without being stirred. Following treatment, cells were taken out, preserved, and prepared in triplicate for microscopy. MIPAR software has been used to analysethe obtained images. RESULTS AND DISCUSSION: Luminescent FAP nanoparticles were synthesized by precipitation and centrifugation at low temperature. The resulting single-phase nanomaterial exhibits cascade fluorescence in the violet and blue regions [2]. To investigate the performance of FAP nanoparticle fluorophores, cells of S. cerevisiae were labeled and observed with a Leica DMIL inverted fluorescence microscope. Nanofluoroapatite fluorophores were successfully labeled S. cerevisiae cells.MIPAR image analysis software extracted the luminescence of nano-biomaterials from yeast cells. CONCLUSIONS: In this study, S. cerevisiae was used as a yeast model which, after labelling with a fluorapatite-based contrast agent, showed luminescent properties. The cascading nature of the agents' luminescence will allow us to monitor cellular uptake as well as monitoring cellular localization in future studies.", publisher = "Beograd : Savez hemijskih inženjera Srbije", journal = "ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, HEMIJSKA INDUSTRIJA (Chemical Industry)", title = "Luminescent fluoroapatite nano-biomaterial for labeling yeast cells as an innovative approach for identification, imaging and monitoring", pages = "67-67", number = "1S", volume = "78" }
Milojkov, D., Jovanović, G.,& Živković-Radovanović, V.. (2024). Luminescent fluoroapatite nano-biomaterial for labeling yeast cells as an innovative approach for identification, imaging and monitoring. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, HEMIJSKA INDUSTRIJA (Chemical Industry) Beograd : Savez hemijskih inženjera Srbije., 78(1S), 67-67.
Milojkov D, Jovanović G, Živković-Radovanović V. Luminescent fluoroapatite nano-biomaterial for labeling yeast cells as an innovative approach for identification, imaging and monitoring. in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, HEMIJSKA INDUSTRIJA (Chemical Industry). 2024;78(1S):67-67..
Milojkov, Dušan, Jovanović, Gvozden, Živković-Radovanović, Vukosava, "Luminescent fluoroapatite nano-biomaterial for labeling yeast cells as an innovative approach for identification, imaging and monitoring" in ExcellMater Conference 2024 - Innovative Biomaterials for Novel Medical Devices, HEMIJSKA INDUSTRIJA (Chemical Industry), 78, no. 1S (2024):67-67.