A single-step electrophoretic deposition (EPD) process from aqueous suspension was used to
assess novel composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS)
and antibiotic, gentamicin (Gent). EPD was carried out at constant voltage on pure titanium
plates. In order to investigate the gentamicin influence on physico-chemical and biological
properties of composite coatings, detailed characterization was performed. For that purpose
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and fieldemission
scanning electron microscopy (FE-SEM) were used. Obtained results confirmed the
formation of a new composite HAP/CS/Gent coating. Potential application was examined
through biological assays. The gentamicin addition strongly improved the antibacterial
activity of HAP/CS/Gent coating that was confirmed by agar diffusion method and test in
suspension. Obtained results indicated more pronounced antibacterial effect against
Staphylococcus a...ureus compared to Escherichia coli. MTT and DET tests indicated low
cytotoxicity against MRC-5 and L929 tested cell lines, indicating good biocompatibility of
obtained composites. MRC-5 human cell line responded better in cytotoxicity testing,
pointing to encouraging results for potential application. The ability of biomineralization
promotion was noticed through the ALP assay. Therefore, HAP/CS/Gent coating can be
considered as perspective material for bone tissue engineering as hard tissue implant.
Source:
Seventeenth Young Researchers Conference – Materials Science and Engineering, 2018, 2-2
Publisher:
Belgrade : Institute of Technical Sciences of SASA
TY - CONF
AU - Stevanović, Milena
AU - Janković, Ana
AU - Đošić, Marija
AU - Vukašinović-Sekulić, Maja
AU - Kojić, Vesna
AU - Mišković-Stanković, Vesna
PY - 2018
UR - https://ritnms.itnms.ac.rs/handle/123456789/770
AB - A single-step electrophoretic deposition (EPD) process from aqueous suspension was used to
assess novel composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS)
and antibiotic, gentamicin (Gent). EPD was carried out at constant voltage on pure titanium
plates. In order to investigate the gentamicin influence on physico-chemical and biological
properties of composite coatings, detailed characterization was performed. For that purpose
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and fieldemission
scanning electron microscopy (FE-SEM) were used. Obtained results confirmed the
formation of a new composite HAP/CS/Gent coating. Potential application was examined
through biological assays. The gentamicin addition strongly improved the antibacterial
activity of HAP/CS/Gent coating that was confirmed by agar diffusion method and test in
suspension. Obtained results indicated more pronounced antibacterial effect against
Staphylococcus aureus compared to Escherichia coli. MTT and DET tests indicated low
cytotoxicity against MRC-5 and L929 tested cell lines, indicating good biocompatibility of
obtained composites. MRC-5 human cell line responded better in cytotoxicity testing,
pointing to encouraging results for potential application. The ability of biomineralization
promotion was noticed through the ALP assay. Therefore, HAP/CS/Gent coating can be
considered as perspective material for bone tissue engineering as hard tissue implant.
PB - Belgrade : Institute of Technical Sciences of SASA
C3 - Seventeenth Young Researchers Conference – Materials Science and Engineering
T1 - Antibiotic loaded bioactive orthopedic implant coating
EP - 2
SP - 2
ER -
@conference{
author = "Stevanović, Milena and Janković, Ana and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Mišković-Stanković, Vesna",
year = "2018",
abstract = "A single-step electrophoretic deposition (EPD) process from aqueous suspension was used to
assess novel composite coating of hydroxyapatite (HAP) with natural polymer chitosan (CS)
and antibiotic, gentamicin (Gent). EPD was carried out at constant voltage on pure titanium
plates. In order to investigate the gentamicin influence on physico-chemical and biological
properties of composite coatings, detailed characterization was performed. For that purpose
X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and fieldemission
scanning electron microscopy (FE-SEM) were used. Obtained results confirmed the
formation of a new composite HAP/CS/Gent coating. Potential application was examined
through biological assays. The gentamicin addition strongly improved the antibacterial
activity of HAP/CS/Gent coating that was confirmed by agar diffusion method and test in
suspension. Obtained results indicated more pronounced antibacterial effect against
Staphylococcus aureus compared to Escherichia coli. MTT and DET tests indicated low
cytotoxicity against MRC-5 and L929 tested cell lines, indicating good biocompatibility of
obtained composites. MRC-5 human cell line responded better in cytotoxicity testing,
pointing to encouraging results for potential application. The ability of biomineralization
promotion was noticed through the ALP assay. Therefore, HAP/CS/Gent coating can be
considered as perspective material for bone tissue engineering as hard tissue implant.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Seventeenth Young Researchers Conference – Materials Science and Engineering",
title = "Antibiotic loaded bioactive orthopedic implant coating",
pages = "2-2"
}
Stevanović, M., Janković, A., Đošić, M., Vukašinović-Sekulić, M., Kojić, V.,& Mišković-Stanković, V.. (2018). Antibiotic loaded bioactive orthopedic implant coating. in Seventeenth Young Researchers Conference – Materials Science and Engineering
Belgrade : Institute of Technical Sciences of SASA., 2-2.
Stevanović M, Janković A, Đošić M, Vukašinović-Sekulić M, Kojić V, Mišković-Stanković V. Antibiotic loaded bioactive orthopedic implant coating. in Seventeenth Young Researchers Conference – Materials Science and Engineering. 2018;:2-2..
Stevanović, Milena, Janković, Ana, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Mišković-Stanković, Vesna, "Antibiotic loaded bioactive orthopedic implant coating" in Seventeenth Young Researchers Conference – Materials Science and Engineering (2018):2-2.
