Trokomponentne bioaktivne prevlake sa gentamicinom elektroforetski taložene na titanu

Abstract

Elektroforetsko taloženje je efikasna elektrohemijska metoda za dobijanje višekomponentnih biokeramičkih prevlaka na metalnom supstratu. Za formiranje biomaterijala za koštane implantate, najbolji rezultati su dobijeni kod prevlaka koje predstavljaju kombinaciju mineralne faze i polimera. U cilju dobijanja bioaktivnih prevlaka na titanu primenom elektroforetskog taloženja, urađena je optimizacija uslova taloženja iz trokomponentne vodene suspenzije hidroksiapatita (HAP), hitozana (CS) i antibiotika gentamicina (Gent), pri različitim konstantnim vrednostima napona i vremena taloženja. Karakterizacija prevlaka je izvršena primenom rendgenske difrakcione analize (XRD) i rendgenske fotoelektronske spektroskopije (XPS). Citotoksičnost dobijenih prevlaka je ispitana MTT testom, dok je antibakterijska aktivnost prevlake sa gentamicinom potvrđena primenom testa u suspenziji. Na osnovu dobijenih rezultata, potvrđeno je da trokomponentna biokompatibilna prevlaka HAP/CS/Gent ima veliki potencijal za upotrebu u biomedicini.

Electrophoretic deposition is a suitable method for obtaining multicomponent bioceramic coatings on titanium (Ti). For hard tissue implants, the best results are achieved for the coating composed of mineral phase and a polymer. First step in producing bioactive coatings on Ti using electrophoretic deposition process was the optimization of deposition parameters for three-component aqueous suspension, containing hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent), at constant values of applied voltages and different deposition times. The coatings obtained were characterized by X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Cytotoxicity of the coatings was evaluated by MTT assay, and the antibacterial activity of gentamicin loaded coating was verified using test in suspension. Based on the experimental results, the formation of a new HAP/CS/Gent composite coating with future prospect for biomedical use was confirmed.