Novel composite coating based on hydroxyapatite, chitosan and gentamicin
(HAP/CS/Gent) was successfully obtained by single-step electrophoretic deposition
process from aqueous suspension. In order to investigate the potential application of
composite HAP/CS/Gent coating, biological properties were examined. The
antibacterial activity of composite coating was tested using an agar diffusion method
and test in suspension against two bacteria strains- Staphylococcus aureus and
Escherichia coli. Biocompatibility was evaluated by cytotoxicity testing, towards MRC-
5 and L929 cell lines using MTT and DET tests. The ability of biomineralization was
tested by ALP assay towards MRC-5 and L929 cell lines as model cells. Excellent
antibacterial properties were observed for HAP/CS/Gent coating, especially in case of
S. aureus. DET and MTT assays indicated low cytotoxicity against MRC-5 and L929
tested cell lines, indicating good biocompatibility. High ALP level in the cell extract
impli...cates the good bioactivity of HAP/CS/Gent coating. Due to the compatibility of
the cells, MRC-5 cell line responded better in ALP assay.
Due to good antibacterial and anti-biofilm efficacy, good biocompatibility and ability to
promote biomineralization, composite HAP/CS/Gent coating can be can be considered
for future medical application as hard tissue implant.
Source:
Šesta konferencija mladih hemičara Srbije, 2018, 102-102
TY - CONF
AU - Stevanović, Milena
AU - Đošić, Marija
AU - Janković, Ana
AU - Mišković-Stanković, Vesna
PY - 2018
UR - https://ritnms.itnms.ac.rs/handle/123456789/763
AB - Novel composite coating based on hydroxyapatite, chitosan and gentamicin
(HAP/CS/Gent) was successfully obtained by single-step electrophoretic deposition
process from aqueous suspension. In order to investigate the potential application of
composite HAP/CS/Gent coating, biological properties were examined. The
antibacterial activity of composite coating was tested using an agar diffusion method
and test in suspension against two bacteria strains- Staphylococcus aureus and
Escherichia coli. Biocompatibility was evaluated by cytotoxicity testing, towards MRC-
5 and L929 cell lines using MTT and DET tests. The ability of biomineralization was
tested by ALP assay towards MRC-5 and L929 cell lines as model cells. Excellent
antibacterial properties were observed for HAP/CS/Gent coating, especially in case of
S. aureus. DET and MTT assays indicated low cytotoxicity against MRC-5 and L929
tested cell lines, indicating good biocompatibility. High ALP level in the cell extract
implicates the good bioactivity of HAP/CS/Gent coating. Due to the compatibility of
the cells, MRC-5 cell line responded better in ALP assay.
Due to good antibacterial and anti-biofilm efficacy, good biocompatibility and ability to
promote biomineralization, composite HAP/CS/Gent coating can be can be considered
for future medical application as hard tissue implant.
PB - Belgrade : Serbian Chemical Society
C3 - Šesta konferencija mladih hemičara Srbije
T1 - Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating
EP - 102
SP - 102
ER -
@conference{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Mišković-Stanković, Vesna",
year = "2018",
abstract = "Novel composite coating based on hydroxyapatite, chitosan and gentamicin
(HAP/CS/Gent) was successfully obtained by single-step electrophoretic deposition
process from aqueous suspension. In order to investigate the potential application of
composite HAP/CS/Gent coating, biological properties were examined. The
antibacterial activity of composite coating was tested using an agar diffusion method
and test in suspension against two bacteria strains- Staphylococcus aureus and
Escherichia coli. Biocompatibility was evaluated by cytotoxicity testing, towards MRC-
5 and L929 cell lines using MTT and DET tests. The ability of biomineralization was
tested by ALP assay towards MRC-5 and L929 cell lines as model cells. Excellent
antibacterial properties were observed for HAP/CS/Gent coating, especially in case of
S. aureus. DET and MTT assays indicated low cytotoxicity against MRC-5 and L929
tested cell lines, indicating good biocompatibility. High ALP level in the cell extract
implicates the good bioactivity of HAP/CS/Gent coating. Due to the compatibility of
the cells, MRC-5 cell line responded better in ALP assay.
Due to good antibacterial and anti-biofilm efficacy, good biocompatibility and ability to
promote biomineralization, composite HAP/CS/Gent coating can be can be considered
for future medical application as hard tissue implant.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Šesta konferencija mladih hemičara Srbije",
title = "Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating",
pages = "102-102"
}
Stevanović, M., Đošić, M., Janković, A.,& Mišković-Stanković, V.. (2018). Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating. in Šesta konferencija mladih hemičara Srbije
Belgrade : Serbian Chemical Society., 102-102.
Stevanović M, Đošić M, Janković A, Mišković-Stanković V. Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating. in Šesta konferencija mladih hemičara Srbije. 2018;:102-102..
Stevanović, Milena, Đošić, Marija, Janković, Ana, Mišković-Stanković, Vesna, "Antibacterial activity and biocompatibility of novel composite hydroxyapatite/chitosan/gentamicin coating" in Šesta konferencija mladih hemičara Srbije (2018):102-102.
