Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala ...dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene.
Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
...Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.
Source:
57. savetovanje Srpskog hemijskog društva, 2021, 46-46
Publisher:
Belgrade : Serbian Chemical Society
Funding / projects:
Funding from European Commisson, project “Twinning to excel materials engineering for medical devices – ExcellMater“ grant no. 952033, H2020-WIDESPREAD- 2018-2020/H2020-WIDESPREAD-2020-5
TY - CONF
AU - Janković, Ana
AU - Stevanović, Milena
AU - Đošić, Marija
AU - Vukašinović-Sekulić, Maja
AU - Kojić, Vesna
AU - Grujić, Svetlana
AU - Matić-Bujagić, Ivana
AU - Mišković-Stanković, Vesna
PY - 2021
UR - https://ritnms.itnms.ac.rs/handle/123456789/759
AB - Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene.
AB - Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.
PB - Belgrade : Serbian Chemical Society
C3 - 57. savetovanje Srpskog hemijskog društva
T1 - Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium
EP - 46
SP - 46
ER -
@conference{
author = "Janković, Ana and Stevanović, Milena and Đošić, Marija and Vukašinović-Sekulić, Maja and Kojić, Vesna and Grujić, Svetlana and Matić-Bujagić, Ivana and Mišković-Stanković, Vesna",
year = "2021",
abstract = "Trenutni trendovi u nauci o biomaterijalima favorizuju dizajniranje multifunkcionalnih prevlaka za
implantate koji služe kao nosači antibakterijskih sredstava sa primarnom namerom da inhibiraju rast
bakterija na površini kontakta implantata i tkiva, a da istovremeno promovišu osteointegraciju.
Elektroforetskim taloženjem (EPD) u jednom koraku pri konstantnom naponu, uspešno je dobijena
kompozitna prevlaka na bazi hidroksiapatita (HAP), hitozana (CS) i gentamicina (Gent) iz vodene
suspenzije na titanu. Kinetika otpuštanja leka ispitivana je u dejonizovanoj vodi na 37 °C tečnom
hromatografijom visokih performansi u kombinaciji sa masenom spektrometrijom. Sposobnost
osteointegracije dalje je potvrđena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na
povoljne osteopromotivne osobine taloženih prevlaka. Kada su testirane na humane i mišje
fibroblastne ćelijske linije (MRC-5 i L929), kompozitna prevlaka pokazala je necitotoksični efekat.
HAP/CS/Gent prevlaka je pokazala dobru antibakterijsku aktivnost prema Staphylococcus aureus i
Escherichia coli, što ukazuje na visok potencijal za biomedicinske primene., Current trends in biomaterials science favor designing multifunctional implant coatings that serve as
carriers of antibacterial agents with the primary intention of inhibiting bacterial growth on the
implant-tissue interface, while still promoting osseointegration. Electrophoretic deposition (EPD) in
a single step at the constant voltage was successfully used to obtain composite coating based on
hydroxyapatite (HAP), chitosan (CS) and gentamicin (Gent) from an aqueous suspension on titanium.
Drug-release kinetics was investigated in deionized water at 37 °C by liquid chromatography coupled
with mass spectrometry. The osseointegration ability was further validated by measuring the alkaline
phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings.
When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings
showed a noncytotoxic effect. HAP/CS/Gent coating exhibited good antibacterial activity against
Staphylococcus aureus and Escherichia coli, indicating the high potential for biomedical applications.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "57. savetovanje Srpskog hemijskog društva",
title = "Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium",
pages = "46-46"
}
Janković, A., Stevanović, M., Đošić, M., Vukašinović-Sekulić, M., Kojić, V., Grujić, S., Matić-Bujagić, I.,& Mišković-Stanković, V.. (2021). Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium. in 57. savetovanje Srpskog hemijskog društva
Belgrade : Serbian Chemical Society., 46-46.
Janković A, Stevanović M, Đošić M, Vukašinović-Sekulić M, Kojić V, Grujić S, Matić-Bujagić I, Mišković-Stanković V. Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium. in 57. savetovanje Srpskog hemijskog društva. 2021;:46-46..
Janković, Ana, Stevanović, Milena, Đošić, Marija, Vukašinović-Sekulić, Maja, Kojić, Vesna, Grujić, Svetlana, Matić-Bujagić, Ivana, Mišković-Stanković, Vesna, "Bioaktivne kompozitne prevlake na titanu koje otpuštaju gentamicin / Bioactive Gentamicin-Eluting Composite Coatings on Titanium" in 57. savetovanje Srpskog hemijskog društva (2021):46-46.
