Elektroforetski taložene kompozitne prevlake kao nosaci lekova / Electrophoretically deposited composite coatings as drug carriers

Abstract

Metoda elektroforetskog taloženja (EPD)se koristi za sintezu biokompatibilnih kompozitnih materijala za medicinsku primenu kao antibakterijske prevlake za koštane implantate. Taloženjem pri konstantnom naponu je uspešno dobijena kompozitna prevlaka hidroksiapatit/hitozan/poli(vinilalkohol) (HAP/CS/PVA) koja sadrži gentamicin (Gent). Bioaktivnost prevlake je dokazana formiranjem novonastalog apatitnog sloja u simuliranoj telesnoj tecnosti i karakterisana primenom skenirajuce elektronske mikroskopije (SEM) I infracrvene spektroskopije sa Furijeovom transformacijom (FTIR). Sposobnost oseointegracije dalje je potvr ena merenjem aktivnosti alkalne fosfataze (ALP), što ukazuje na povoljna oseopromotivna svojstva taloženih prevlaka. Kada su testirane humane i mišje fibroblastne celijske linije (MRC-5 i L929), kompozitne prevlake nisu pokazale citotoksicni efekat

The electrophoretic deposition (EPD) method isused for the synthesis of biocompatible composite materials for medical use as antibacterial coatings for bone implants. Deposition at the constant voltage yielded composite coating hydroxyapatite/chitosan/poly(vinyl alcohol)(HAP/CS/PVA) loaded with gentamicin (Gent). Bioactivity of the coating was proved by a newly formed apatite layer in simulated body fluid and characterized usingscanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The osseointegration ability was validated by measuring the alkaline phosphatase activity (ALP) indicating the favorable osseopromotive properties of deposited coatings. When tested against human and mice fibroblast cells lines (MRC-5 and L929), composite coatings did not exhibit cytotoxic effect.