Efikasnost organozeolita u preveniranju toksičnih efekata zearalenona kod jagnjadi

Abstract

Efikasnost adsorpcije zearalenona (ZEN) na mineralnom adsorbentu mikotoksina dobijenim organskom modifikacijom prirodnog zeolita (Min-Zel Plus®) je praćena u uslovima in vitro i ogledima in vivo na jagnjadima koja su dobijala hranu kontaminiranu ZEN-om. In vitro rezultati su pokazali da Min-Zel Plus® adsorbuje ZEN sa indeksom adsorpcije 90%, na pH 3, 7 i 9. Za in vivo eksperimente jagnjad su podeljena u četiri grupe, u svakoj grupi po 16 jagnjadi: I grupa - kontrolna je hranjena hranom koja ne sadrži ni ZEN ni Min-Zel Plus®, II grupa - je dobijala hanu kontaminiranu sa 8.3 mg ZEN/kg III grupa je dobijala hranu kontaminiranu sa 8.3 mgZEN/kg i 0.2% Min-a-Zel-a Plus® i IV grupa je dobijala hranu kontaminiranu sa 8.3 mg ZEN/kg i 0.5% Min-Zel-a Plus®. Rezultati analiza sadržaja ZEN u organima jagnjadi su pokazala da je za razliku od II grupe u kojoj je značajno prisutan ZEN u svim organima, u grupama III i IV, koje su dobijale i Min-Zel-a Plus® značajno je smanjen sadržaj ZEN u jetri, bubrezima i mišićima. Ipak, pri količini od 0.2% Min- Zel-a Plus® u ispitivanim organima jagnjadi prisutne su male količine zaostalog ZEN, dok pri količini od 0.5% nije detektovan ZEN u ispitivanim organima.

In vitro and in vivo studies were conducted to evaluate the efficacy of organozeolite (Min-a-Zel Plus®) to alleviate the toxic effects of zearalenone (ZEN) in lambs. Min-a-Zel Plus® was able to bind >90% of ZEN in vitro at pH 3, 7 and 9. For in vivo studies, sixty four lambs were divided in to four groups which included: I - control group - basal diet containing neither Min-a-Zel Plus® nor zearalenone; II - basal diet supplemented with 8.3 mg ZEN/kg diet; III - basal diet supplemented with 8.3 mg ZEN/kg and 0.2% Min-a-Zel Plus®; IV - basal diet supplemented with 8.3 mg ZEN/kg and 0.5% Min-a-Zel Plus®. The Min-a-Zel Plus® supplement dramatically reduced the content of ZEN in liver, kidneys and muscles. The lower amount (0.2%) reduced the content of ZEN in all samples, but ZEN was still present in the organs. Addition of 0.5% Min-a-Zel Plus® eliminated ZEN from all organs, totally. These results indicate that Min-a-Zel Plus® is effective in preventing the toxic effects of ZEN, which may be present in lamb rations.