Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium
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2018
Authors
Stevanović, Milena
Đošić, Marija

Janković, Ana

Kojić, Vesna

Vukasinović-Sekulić, Maja

Stojanović, Jovica

Odović, Jadranka
Crevar-Sakac, Milkica
Rhee, Kyong Yop

Mišković-Stanković, Vesna

Article (Published version)

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Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed a...gainst Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.
Keywords:
surface analysis / electrophoretic deposition / coatings / biocomposite / antibacterial activitySource:
ACS Biomaterials Science & Engineering, 2018, 4, 12, 3994-4007Publisher:
- Amer Chemical Soc, Washington
Funding / projects:
- Basic Science Research Program of the Ministry of Education, Science and Technology of Korea [2018R1A2B5A02023190]
- Synthesis, processing and applications of nanostructured multifunctional materials with defined properties (RS-45019)
DOI: 10.1021/acsbiomaterials.8b00859
ISSN: 2373-9878
PubMed: 33418800
WoS: 000453109000008
Scopus: 2-s2.0-85058300298
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Institution/Community
Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - JOUR AU - Stevanović, Milena AU - Đošić, Marija AU - Janković, Ana AU - Kojić, Vesna AU - Vukasinović-Sekulić, Maja AU - Stojanović, Jovica AU - Odović, Jadranka AU - Crevar-Sakac, Milkica AU - Rhee, Kyong Yop AU - Mišković-Stanković, Vesna PY - 2018 UR - https://ritnms.itnms.ac.rs/handle/123456789/468 AB - Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic. PB - Amer Chemical Soc, Washington T2 - ACS Biomaterials Science & Engineering T1 - Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium EP - 4007 IS - 12 SP - 3994 VL - 4 DO - 10.1021/acsbiomaterials.8b00859 UR - conv_839 ER -
@article{ author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukasinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakac, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna", year = "2018", abstract = "Composite coating of antibiotic gentamicin (Gent), natural polymer chitosan (CS), and hydroxyapatite (HAP) was successfully assessed by applying the electrophoretic deposition (EPD) technique. EPD was performed under optimized deposition conditions (5 V, 12 min) on pure titanium plates, to obtain HAP/CS and HAP/CS/Gent composite coatings in a single step from three-component aqueous suspension, with favorable antibacterial properties. Composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis, confirming the formation of composite HAP/CS and HAP/CS/Gent coatings on the titanium surface, which is due to intermolecular hydrogen bonds. Employing the XRD technique, HAP was detected by obtaining the characteristic diffraction maximums. Good antibacterial activity of the composite coating loaded with antibiotic (HAP/CS/Gent) was confirmed against Staphylococcus aureus and Escherichia coli, pointing to the high potential for bioapplication. Introduction of gentamicin in HAP/CS/Gent coating caused very mild cytotoxicity in the tested cell lines MRC-5 and L929. MTT testing was used to evaluate cell viability, and HAP/CS was classified as noncytotoxic.", publisher = "Amer Chemical Soc, Washington", journal = "ACS Biomaterials Science & Engineering", title = "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium", pages = "4007-3994", number = "12", volume = "4", doi = "10.1021/acsbiomaterials.8b00859", url = "conv_839" }
Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukasinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakac, M., Rhee, K. Y.,& Mišković-Stanković, V.. (2018). Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering Amer Chemical Soc, Washington., 4(12), 3994-4007. https://doi.org/10.1021/acsbiomaterials.8b00859 conv_839
Stevanović M, Đošić M, Janković A, Kojić V, Vukasinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakac M, Rhee KY, Mišković-Stanković V. Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium. in ACS Biomaterials Science & Engineering. 2018;4(12):3994-4007. doi:10.1021/acsbiomaterials.8b00859 conv_839 .
Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukasinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakac, Milkica, Rhee, Kyong Yop, Mišković-Stanković, Vesna, "Gentamicin-Loaded Bioactive Hydroxyapatite/Chitosan Composite Coating Electrodeposited on Titanium" in ACS Biomaterials Science & Engineering, 4, no. 12 (2018):3994-4007, https://doi.org/10.1021/acsbiomaterials.8b00859 ., conv_839 .