In vitro ocena koštice breskve kao adsorbensa mikotoksina

Abstract

Rad opisuje in vitro model za procenu sposobnosti koštice breskve (nemodifikovane i modifikovane), pripremljene u Institutu za tehnologiju nuklearnih i drugih mineralnih sirovina u Beogradu, da adsorbuje različite mikotoksine. Koštice breskve su nabavljene od 'Vino Župe' iz Aleksandrovca, kompanije gde su odložene kao otpadni materijal njihove Fabrike sokova. Nakon odgovarajuće pripreme, u ispitivanju su korišćene dve vrste čestica koštice breskve: jedna nemodifikovana (PS) i druga dobijena kiselinskom modifikacijom (MPS). In vitro metodom je testirana adsorpcija šest mikotoksina: aflatoksina B1 (AFL), ohratoksina A (OTA), deoksinivalenola (DON), zearalenona (ZON), di-acetokiscirpenola (DAS) i T-2 toksina. Za eksperimenate adsorpcije korišćeni su sirovi ekstrakti mikotoksina proizvedeni u Odeljenju za mikrobiologiju Bio-ekološkog centra u Zrenjaninu. Sposobnost čestica nemodifikovane i modifikovane koštice breskve za vezivanje mikotoksina je ocenjivana u elektrolitu 0,1 M K2HPO4 čija je pH vrednost podešena na 3,0 odnosno 7,0. Maseni odnos pojedinačnih mikotoksina i uzoraka koštice breskve je bio 1:5000. Eksperimentalne smeše su inkubirane 1 sat na rotacionoj tresilici (185 o/min) na sobnoj temperaturi (22-25ºC). Posle inkubacije, ekstrakcija neadsorbovanih mikotoksina iz filtrata eksperimentalnih smeša su izvršene organskim rastvaračima, a njihova kvantifikacija metodom tankoslojne hromatografije.

The paper describes in vitro model for the evaluation of ability of peach shell (unmodified and modified), prepared at the Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, to adsorb different mycotoxins. Peach stones were obtained from 'Vino Župa' Company from Aleksandrovac, where they have been disposed of as by-products from their Juice Factory. After proper preparation, two sorts of peach shell particles were used: one as unmodified peach shell particles (PS) and another one obtained by acid modification, denoted as MPS. Adsorption of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin by PS and MPS was tested in vitro. Crude extracts of mycotoxins, produced at the Department of Microbiology of Bio-ecological Center, Zrenjanin, were used for adsorption experiments. The ability of binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4, which pH value was adjusted to 3.0 and 7.0, respectively. Mass ratio of individual mycotoxin and peach shell samples was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25ºC). After incubation, the extractions of non-adsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantification was done by thin-layer chromatography (TLC).