TY  - CONF
AU  - Milojkov, Dušan
AU  - Manojlović, Vaso
AU  - Nastasijević, Branislav
AU  - Sokić, Miroslav
PY  - 2022
UR  - https://ritnms.itnms.ac.rs/handle/123456789/934
AB  - Luminescent inorganic nanomaterials (nanophosphorus) based on fluorapatite (FAP) are attracting significant interest for biomedical, biotechnological and environmental applications. 
Luminescent nanopowders based on fluorapatite substituted with Pr3+ and CO32-ions (PrCFAP) were obtained by a simple precipitation method. Previously published results have shown that nanopowders are monophasic and composed of nanospheres, with luminescence in violet color. Also, 
nanopowders exhibit and antibacterial behavior against E. coli. In this study the results of chemocompatibility of antibacterial luminescent nanophospors obtained by precipitation for various biomedical and health applications are presented. Pure FAP showed a degree of hemolysis of 5% and can be considered chemcompatible. Doped nanopowders showed hemolysis up to about 10%. Slight hemolytic behavior of the tested PrCFAP nanopowders may be a consequence of mechanical stress of the cell in the interaction with nanoparticles, as well as the toxic effect of dissolved Pr3+ and 
F- ions from the FAP lattice. The above results indicate that the antibacterial PrCFAP nanophosphorus may be future evaluated for different biomedical applications, such as agents for cells labeling, therapy and drug delivery.
AB  - Luminescentni neorganski nanomaterijali (nanofosfor) na bazi fluorapatita (FAP) privlače značajno interesovanje za biomedicinske, biotehnološke i ekološke primene. Jednostavnom metodom 
precipitacije dobijeni su luminescentni nanomaterijali na bazi fluorapatita supstituisani Pr3+ i CO32- jonima (PrCFAP). Ranije objavljeni rezultati su pokazali da su nanoprahovi monofazni i sastavljeni od nanosfera, sa luminiscencijom u ljubičastoj boji. Pored toga, nanoprahovi su pokazali i antibakterijsko ponašanje protiv E. coli. U ovoj studiji prikazani su rezultati hemokompatibilnosti antibakterijskih luminiscentnih nanofospora dobijenih precipitacijom za različite biomedicinske i zdravstvene primene. Čisti FAP je pokazao stepen hemolize od 5% i može se smatrati hemokompatibilnim. Dopirani nanoprahovi su pokazali hemolizu do oko 10%. Blago hemolitičko ponašanje ispitivanih PrCFAP nanoprahova može biti posledica mehaničkog naprezanja ćelije u interakciji sa nanočesticama, kao i toksičnog dejstva rastvorenih Pr3+ i F jona iz FAP rešetke. Gore navedeni rezultati ukazuju da antibakterijski PrCFAP nanofosfori u budućnosti mogu biti procenjeni za različite biomedicinske primene, kao što su agenti za obeležavanje ćelija, terapije i isporuku lekova.
PB  - Belgrade : Serbian Society of Corrosion and Materials Protection UISKOZAM
C3  - XXIII YuCorr International Conference. Meeting point of the science and practice in the fields of corrosion, materials and environmental protection
T1  - Chemocompatibility of fluorapatite-based antibacterial  nanophosphorus prepared by precipitation method for biomedical  applications
T1  - Hemokompatibilnost antibakterijskih nanofosfora na bazi fluorapatita  pripremljenog metodom precipitacije za biomedicinsku primenu
EP  - 42
SP  - 37
ER  - 

@conference{
author = "Milojkov, Dušan and Manojlović, Vaso and Nastasijević, Branislav and Sokić, Miroslav",
year = "2022",
abstract = "Luminescent inorganic nanomaterials (nanophosphorus) based on fluorapatite (FAP) are attracting significant interest for biomedical, biotechnological and environmental applications. 
Luminescent nanopowders based on fluorapatite substituted with Pr3+ and CO32-ions (PrCFAP) were obtained by a simple precipitation method. Previously published results have shown that nanopowders are monophasic and composed of nanospheres, with luminescence in violet color. Also, 
nanopowders exhibit and antibacterial behavior against E. coli. In this study the results of chemocompatibility of antibacterial luminescent nanophospors obtained by precipitation for various biomedical and health applications are presented. Pure FAP showed a degree of hemolysis of 5% and can be considered chemcompatible. Doped nanopowders showed hemolysis up to about 10%. Slight hemolytic behavior of the tested PrCFAP nanopowders may be a consequence of mechanical stress of the cell in the interaction with nanoparticles, as well as the toxic effect of dissolved Pr3+ and 
F- ions from the FAP lattice. The above results indicate that the antibacterial PrCFAP nanophosphorus may be future evaluated for different biomedical applications, such as agents for cells labeling, therapy and drug delivery., Luminescentni neorganski nanomaterijali (nanofosfor) na bazi fluorapatita (FAP) privlače značajno interesovanje za biomedicinske, biotehnološke i ekološke primene. Jednostavnom metodom 
precipitacije dobijeni su luminescentni nanomaterijali na bazi fluorapatita supstituisani Pr3+ i CO32- jonima (PrCFAP). Ranije objavljeni rezultati su pokazali da su nanoprahovi monofazni i sastavljeni od nanosfera, sa luminiscencijom u ljubičastoj boji. Pored toga, nanoprahovi su pokazali i antibakterijsko ponašanje protiv E. coli. U ovoj studiji prikazani su rezultati hemokompatibilnosti antibakterijskih luminiscentnih nanofospora dobijenih precipitacijom za različite biomedicinske i zdravstvene primene. Čisti FAP je pokazao stepen hemolize od 5% i može se smatrati hemokompatibilnim. Dopirani nanoprahovi su pokazali hemolizu do oko 10%. Blago hemolitičko ponašanje ispitivanih PrCFAP nanoprahova može biti posledica mehaničkog naprezanja ćelije u interakciji sa nanočesticama, kao i toksičnog dejstva rastvorenih Pr3+ i F jona iz FAP rešetke. Gore navedeni rezultati ukazuju da antibakterijski PrCFAP nanofosfori u budućnosti mogu biti procenjeni za različite biomedicinske primene, kao što su agenti za obeležavanje ćelija, terapije i isporuku lekova.",
publisher = "Belgrade : Serbian Society of Corrosion and Materials Protection UISKOZAM",
journal = "XXIII YuCorr International Conference. Meeting point of the science and practice in the fields of corrosion, materials and environmental protection",
title = "Chemocompatibility of fluorapatite-based antibacterial  nanophosphorus prepared by precipitation method for biomedical  applications, Hemokompatibilnost antibakterijskih nanofosfora na bazi fluorapatita  pripremljenog metodom precipitacije za biomedicinsku primenu",
pages = "42-37"
}

Milojkov, D., Manojlović, V., Nastasijević, B.,& Sokić, M.. (2022). Chemocompatibility of fluorapatite-based antibacterial  nanophosphorus prepared by precipitation method for biomedical  applications. in XXIII YuCorr International Conference. Meeting point of the science and practice in the fields of corrosion, materials and environmental protection
Belgrade : Serbian Society of Corrosion and Materials Protection UISKOZAM., 37-42.

Milojkov D, Manojlović V, Nastasijević B, Sokić M. Chemocompatibility of fluorapatite-based antibacterial  nanophosphorus prepared by precipitation method for biomedical  applications. in XXIII YuCorr International Conference. Meeting point of the science and practice in the fields of corrosion, materials and environmental protection. 2022;:37-42..

Milojkov, Dušan, Manojlović, Vaso, Nastasijević, Branislav, Sokić, Miroslav, "Chemocompatibility of fluorapatite-based antibacterial  nanophosphorus prepared by precipitation method for biomedical  applications" in XXIII YuCorr International Conference. Meeting point of the science and practice in the fields of corrosion, materials and environmental protection (2022):37-42.

TY  - CONF
AU  - Milojkov, Dušan
AU  - Nastasijević, Branislav
AU  - Živković-Radovanović, Vukosava
AU  - Janjić, Goran
AU  - Dimović, Slavko
AU  - Mutavdžić, Dragosav
AU  - Stanić, Vojislav
PY  - 2018
UR  - https://ritnms.itnms.ac.rs/handle/123456789/967
AB  - In this study, the preparation and characterization of luminescent
fluorapatite (FAP) nanoparticles (50 and 80 nm rod-size), and their use for
labeling of Saccharomyces cerevisae cells were investigated. Fluorapatite
nanoparticles were obtained by neutralization method. X-ray diffraction
(XRD), scanning electron microscopy (SEM) and photoluminescence (PL)
methods have confirmed a rod-like material of nanometer size with
broadband emission within 380-600 nm under UVA light excitation.
Fluorescence microscopy showed that fluorapatite nano-fluorophors
successfully labeled S. cerevisiae cells, by attaching the proteins on the cell
wall. Obtained luminescent fluorapatite nanorods could be used as a probe
for visualization and detection of yeast cells.
C3  - 6th Workshop: Specific methods for food safety and quality, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Synthesis and characterization of luminescent fluorapatite nanorods for labelling of Saccharomyces cerevisiae cells
EP  - 162
SP  - 159
ER  - 

@conference{
author = "Milojkov, Dušan and Nastasijević, Branislav and Živković-Radovanović, Vukosava and Janjić, Goran and Dimović, Slavko and Mutavdžić, Dragosav and Stanić, Vojislav",
year = "2018",
abstract = "In this study, the preparation and characterization of luminescent
fluorapatite (FAP) nanoparticles (50 and 80 nm rod-size), and their use for
labeling of Saccharomyces cerevisae cells were investigated. Fluorapatite
nanoparticles were obtained by neutralization method. X-ray diffraction
(XRD), scanning electron microscopy (SEM) and photoluminescence (PL)
methods have confirmed a rod-like material of nanometer size with
broadband emission within 380-600 nm under UVA light excitation.
Fluorescence microscopy showed that fluorapatite nano-fluorophors
successfully labeled S. cerevisiae cells, by attaching the proteins on the cell
wall. Obtained luminescent fluorapatite nanorods could be used as a probe
for visualization and detection of yeast cells.",
journal = "6th Workshop: Specific methods for food safety and quality, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Synthesis and characterization of luminescent fluorapatite nanorods for labelling of Saccharomyces cerevisiae cells",
pages = "162-159"
}

Milojkov, D., Nastasijević, B., Živković-Radovanović, V., Janjić, G., Dimović, S., Mutavdžić, D.,& Stanić, V.. (2018). Synthesis and characterization of luminescent fluorapatite nanorods for labelling of Saccharomyces cerevisiae cells. in 6th Workshop: Specific methods for food safety and quality, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 159-162.

Milojkov D, Nastasijević B, Živković-Radovanović V, Janjić G, Dimović S, Mutavdžić D, Stanić V. Synthesis and characterization of luminescent fluorapatite nanorods for labelling of Saccharomyces cerevisiae cells. in 6th Workshop: Specific methods for food safety and quality, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2018;:159-162..

Milojkov, Dušan, Nastasijević, Branislav, Živković-Radovanović, Vukosava, Janjić, Goran, Dimović, Slavko, Mutavdžić, Dragosav, Stanić, Vojislav, "Synthesis and characterization of luminescent fluorapatite nanorods for labelling of Saccharomyces cerevisiae cells" in 6th Workshop: Specific methods for food safety and quality, 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2018):159-162.

TY  - CONF
AU  - Milojkov, Dušan
AU  - Stanić, Vojislav
AU  - Janjić, Goran
AU  - Mutavdžić, Dragosav
AU  - Nastasijević, Branislav
AU  - Radosavljević-Mihajlović, Ana
AU  - Milanović, Marija
PY  - 2017
UR  - https://ritnms.itnms.ac.rs/handle/123456789/961
AB  - Nanočestice apatita dopirane elementima retkih zemalja su intenzivno proučavane kao potencijalni luminiscentni materijali za obeležavanje ćelija i tkiva, u bioinženjeringu tkiva, za remodeliranje kostiju i posmatranje distribucije lekova [1-3]. Nanočestice Pr3+ dopiranih fluorapatita (Pr-FAP) sfernog oblika uspešno su sintetisane postupkom koprecipitacije i okarakterisane primenom XRD, FTIR, PL i SEM-EDX metoda. Emisija nanočestica fluorapatita (FAP) javlja se u ljubičastoj oblasti 
vidljivog dela spektra, uz crveni pomak u oblast zelene boje kada je Pr3+ ugrađen u rešetku. MCR-ALS (Multivariate Curve Resolution-Alternating Least Squares) analiza fluorescentnog spektara i ab initio proračuni ukazuju da se joni Pr3+ nalaze u Pr-FAP rešetki na Ca2 (6h) položaju. Pretpostavljamo da je mehanizam zamene Ca2+ jona sa Pr3+ jonima praćen kompenzacijom naelektrisanja ugrađivanjem CO32- jona u strukturi ili 
formiranjem šupljina na položajima jona metala. Dobijeni uzorci pokazali su mali stepen hemolize, a stepen hemolize se povećava sa smanjenjem veličine kristalita. Rezultati hemolize ukazuju da se ove sintetičke
nanočestice mogu potencijalno koristiti za dalja biomedicinska istraživanja, kao što su obeležavanje ćelija i tkiva, bioinženjering, za terapiju karcinoma i ispitivanje distribucije leka.
AB  - The apatite nanoparticles doped with rear earth elements have been extensively studied as a potential luminescence inorganic material for cells and tissue labeling, bioimaging in tissue engineering and bone re-modelling, and for observing the distribution and drug delivery [1-3]. Sphere-like nanoparticles of Pr3+ doped fluorapatite (Pr-FAP) have been successfully 
synthesized by co-precipitation method and characterized by XRD, FTIR, PL and SEM EDX methods. Emission of fluorapatite nanoparticles (FAP) occurs in violet region of visible part of spectrum, with red shift to green color region when Pr3+ is doped in lattice. MCR-ALS (Multivariate Curve Resolution-Alternating Least Squares) analyses of fluorescence spectra and ab initio calculation indicated that Pr3+ ions are located in Pr FAP lattice at Ca2 (6h) sites. We assume that the mechanism of substitution of Ca2+ with Pr3+ ion is accompanied with charge compensation by incorporation of CO32- ions in structure or by vacancies formation on metal ions position. The obtained samples showed a small degree of hemolysis, and degree of hemolysis 
increases with decrease in crystallite size. The results of hemolysis suggest that the synthesized nanoparticles can be potentially used for further biomedical research, such as cells and tissue labeling, bioimaging, cancer therapy and drug delivery.
PB  - Belgrade : Serbian Ceramic Society
C3  - XXIV Kонференција српског кристалографског друштва
T1  - Nanočestice Pr3+ dopiranih fluorapatita dobijenih koprecipitacionom metodom
T1  - Pr3+ doped fluorapatite nanoparticles obtained by co-precipitation method
EP  - 19
SP  - 18
ER  - 

@conference{
author = "Milojkov, Dušan and Stanić, Vojislav and Janjić, Goran and Mutavdžić, Dragosav and Nastasijević, Branislav and Radosavljević-Mihajlović, Ana and Milanović, Marija",
year = "2017",
abstract = "Nanočestice apatita dopirane elementima retkih zemalja su intenzivno proučavane kao potencijalni luminiscentni materijali za obeležavanje ćelija i tkiva, u bioinženjeringu tkiva, za remodeliranje kostiju i posmatranje distribucije lekova [1-3]. Nanočestice Pr3+ dopiranih fluorapatita (Pr-FAP) sfernog oblika uspešno su sintetisane postupkom koprecipitacije i okarakterisane primenom XRD, FTIR, PL i SEM-EDX metoda. Emisija nanočestica fluorapatita (FAP) javlja se u ljubičastoj oblasti 
vidljivog dela spektra, uz crveni pomak u oblast zelene boje kada je Pr3+ ugrađen u rešetku. MCR-ALS (Multivariate Curve Resolution-Alternating Least Squares) analiza fluorescentnog spektara i ab initio proračuni ukazuju da se joni Pr3+ nalaze u Pr-FAP rešetki na Ca2 (6h) položaju. Pretpostavljamo da je mehanizam zamene Ca2+ jona sa Pr3+ jonima praćen kompenzacijom naelektrisanja ugrađivanjem CO32- jona u strukturi ili 
formiranjem šupljina na položajima jona metala. Dobijeni uzorci pokazali su mali stepen hemolize, a stepen hemolize se povećava sa smanjenjem veličine kristalita. Rezultati hemolize ukazuju da se ove sintetičke
nanočestice mogu potencijalno koristiti za dalja biomedicinska istraživanja, kao što su obeležavanje ćelija i tkiva, bioinženjering, za terapiju karcinoma i ispitivanje distribucije leka., The apatite nanoparticles doped with rear earth elements have been extensively studied as a potential luminescence inorganic material for cells and tissue labeling, bioimaging in tissue engineering and bone re-modelling, and for observing the distribution and drug delivery [1-3]. Sphere-like nanoparticles of Pr3+ doped fluorapatite (Pr-FAP) have been successfully 
synthesized by co-precipitation method and characterized by XRD, FTIR, PL and SEM EDX methods. Emission of fluorapatite nanoparticles (FAP) occurs in violet region of visible part of spectrum, with red shift to green color region when Pr3+ is doped in lattice. MCR-ALS (Multivariate Curve Resolution-Alternating Least Squares) analyses of fluorescence spectra and ab initio calculation indicated that Pr3+ ions are located in Pr FAP lattice at Ca2 (6h) sites. We assume that the mechanism of substitution of Ca2+ with Pr3+ ion is accompanied with charge compensation by incorporation of CO32- ions in structure or by vacancies formation on metal ions position. The obtained samples showed a small degree of hemolysis, and degree of hemolysis 
increases with decrease in crystallite size. The results of hemolysis suggest that the synthesized nanoparticles can be potentially used for further biomedical research, such as cells and tissue labeling, bioimaging, cancer therapy and drug delivery.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "XXIV Kонференција српског кристалографског друштва",
title = "Nanočestice Pr3+ dopiranih fluorapatita dobijenih koprecipitacionom metodom, Pr3+ doped fluorapatite nanoparticles obtained by co-precipitation method",
pages = "19-18"
}

Milojkov, D., Stanić, V., Janjić, G., Mutavdžić, D., Nastasijević, B., Radosavljević-Mihajlović, A.,& Milanović, M.. (2017). Nanočestice Pr3+ dopiranih fluorapatita dobijenih koprecipitacionom metodom. in XXIV Kонференција српског кристалографског друштва
Belgrade : Serbian Ceramic Society., 18-19.

Milojkov D, Stanić V, Janjić G, Mutavdžić D, Nastasijević B, Radosavljević-Mihajlović A, Milanović M. Nanočestice Pr3+ dopiranih fluorapatita dobijenih koprecipitacionom metodom. in XXIV Kонференција српског кристалографског друштва. 2017;:18-19..

Milojkov, Dušan, Stanić, Vojislav, Janjić, Goran, Mutavdžić, Dragosav, Nastasijević, Branislav, Radosavljević-Mihajlović, Ana, Milanović, Marija, "Nanočestice Pr3+ dopiranih fluorapatita dobijenih koprecipitacionom metodom" in XXIV Kонференција српског кристалографског друштва (2017):18-19.

TY  - JOUR
AU  - Stanić, Vojislav
AU  - Radosavljević-Mihajlović, Ana
AU  - Živković-Radovanović, Vukosava
AU  - Nastasijević, Branislav
AU  - Marković, Jelena
AU  - Budimir, Milica
PY  - 2015
UR  - https://ritnms.itnms.ac.rs/handle/123456789/998
AB  - Silver doped fluorapatite nanopowders were synthesised by neutralization method, which consists of dissolving Ag2O in solution of HF and H3PO4 and addition to suspension of Ca(OH)2. The powder XRD, SEM and FTIR studies indicated the formation of a fluorapatite nanomaterials with average length of the particles is about 80 nm and a width of about 15 nm. The FTIR studies show that carbonate content in samples is very small and carbonte ions substitute both phosphate and hydroxyl groups in the crystal structure of samples, forming AB-type fluorapatite. Antibacterial studies have demonstrated that all Ag+-doped fluorapatite samples exhibit bactericidal effect against pathogens: Staphylococcus aureus, Micrococcus luteus and Kllebsiela pneumoniae. Antibacterial activity increased with the increase of Ag+ in the samples. The atomic force microscopy studies revealed extensive damage to the bacterial cell envelops in the presence of Ag+-doped fluorapatite particles which may lead to their death. The synthesized Ag+-doped fluorapatite nanomaterials are promising as antibacterial biomaterials in orthopedics and dentistry.
PB  - Elsevier B.V.
T2  - Applied Surface Science
T1  - Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method
EP  - 80
IS  - 15
SP  - 72
VL  - 337
DO  - 10.1016/j.apsusc.2015.02.065
ER  - 

@article{
author = "Stanić, Vojislav and Radosavljević-Mihajlović, Ana and Živković-Radovanović, Vukosava and Nastasijević, Branislav and Marković, Jelena and Budimir, Milica",
year = "2015",
abstract = "Silver doped fluorapatite nanopowders were synthesised by neutralization method, which consists of dissolving Ag2O in solution of HF and H3PO4 and addition to suspension of Ca(OH)2. The powder XRD, SEM and FTIR studies indicated the formation of a fluorapatite nanomaterials with average length of the particles is about 80 nm and a width of about 15 nm. The FTIR studies show that carbonate content in samples is very small and carbonte ions substitute both phosphate and hydroxyl groups in the crystal structure of samples, forming AB-type fluorapatite. Antibacterial studies have demonstrated that all Ag+-doped fluorapatite samples exhibit bactericidal effect against pathogens: Staphylococcus aureus, Micrococcus luteus and Kllebsiela pneumoniae. Antibacterial activity increased with the increase of Ag+ in the samples. The atomic force microscopy studies revealed extensive damage to the bacterial cell envelops in the presence of Ag+-doped fluorapatite particles which may lead to their death. The synthesized Ag+-doped fluorapatite nanomaterials are promising as antibacterial biomaterials in orthopedics and dentistry.",
publisher = "Elsevier B.V.",
journal = "Applied Surface Science",
title = "Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method",
pages = "80-72",
number = "15",
volume = "337",
doi = "10.1016/j.apsusc.2015.02.065"
}

Stanić, V., Radosavljević-Mihajlović, A., Živković-Radovanović, V., Nastasijević, B., Marković, J.,& Budimir, M.. (2015). Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method. in Applied Surface Science
Elsevier B.V.., 337(15), 72-80.
https://doi.org/10.1016/j.apsusc.2015.02.065

Stanić V, Radosavljević-Mihajlović A, Živković-Radovanović V, Nastasijević B, Marković J, Budimir M. Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method. in Applied Surface Science. 2015;337(15):72-80.
doi:10.1016/j.apsusc.2015.02.065 .

Stanić, Vojislav, Radosavljević-Mihajlović, Ana, Živković-Radovanović, Vukosava, Nastasijević, Branislav, Marković, Jelena, Budimir, Milica, "Synthesis, structural characterisation and antibacterial activity of Ag+-doped fluorapatite nanomaterials prepared by neutralization method" in Applied Surface Science, 337, no. 15 (2015):72-80,
https://doi.org/10.1016/j.apsusc.2015.02.065 . .