@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Rhee, Kyong Yop and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining
graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan
(CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process
was performed as a single step process at a constant voltage (5 V, deposition
time 12 min) on pure titanium foils. The influence of graphene was examined through
detailed physicochemical and biological characterization. Fourier transform infrared
spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis,
X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation
of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained
coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial
interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong
bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin
addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating
that was confirmed by antibacterial activity kinetics in suspension and agar diffusion
testing, while results indicated more pronounced antibacterial effect against Staphylococcus
aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared
to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated
low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability)
tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating.
Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a
prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley",
journal = "Journal of Biomedical Materials Research",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
pages = "2189-2175",
number = "11",
volume = "108",
doi = "10.1002/jbm.a.36974"
}