TY  - JOUR
AU  - Lopičić, Zorica
AU  - Bočarov-Stančić, Aleksandra
AU  - Stojanović, Mirjana
AU  - Milojković, Jelena
AU  - Pantić, Vladimir R.
AU  - Adamović, Milan
PY  - 2013
UR  - https://ritnms.itnms.ac.rs/handle/123456789/270
AB  - Rad opisuje in vitro model za procenu sposobnosti koštice breskve (nemodifikovane i modifikovane), pripremljene u Institutu za tehnologiju nuklearnih i drugih mineralnih sirovina u Beogradu, da adsorbuje različite mikotoksine. Koštice breskve su nabavljene od 'Vino Župe' iz Aleksandrovca, kompanije gde su odložene kao otpadni materijal njihove Fabrike sokova. Nakon odgovarajuće pripreme, u ispitivanju su korišćene dve vrste čestica koštice breskve: jedna nemodifikovana (PS) i druga dobijena kiselinskom modifikacijom (MPS). In vitro metodom je testirana adsorpcija šest mikotoksina: aflatoksina B1 (AFL), ohratoksina A (OTA), deoksinivalenola (DON), zearalenona (ZON), di-acetokiscirpenola (DAS) i T-2 toksina. Za eksperimenate adsorpcije korišćeni su sirovi ekstrakti mikotoksina proizvedeni u Odeljenju za mikrobiologiju Bio-ekološkog centra u Zrenjaninu. Sposobnost čestica nemodifikovane i modifikovane koštice breskve za vezivanje mikotoksina je ocenjivana u elektrolitu 0,1 M K2HPO4 čija je pH vrednost podešena na 3,0 odnosno 7,0. Maseni odnos pojedinačnih mikotoksina i uzoraka koštice breskve je bio 1:5000. Eksperimentalne smeše su inkubirane 1 sat na rotacionoj tresilici (185 o/min) na sobnoj temperaturi (22-25ºC). Posle inkubacije, ekstrakcija neadsorbovanih mikotoksina iz filtrata eksperimentalnih smeša su izvršene organskim rastvaračima, a njihova kvantifikacija metodom tankoslojne hromatografije.
AB  - The paper describes in vitro model for the evaluation of ability of peach shell (unmodified and modified), prepared at the Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, to adsorb different mycotoxins. Peach stones were obtained from 'Vino Župa' Company from Aleksandrovac, where they have been disposed of as by-products from their Juice Factory. After proper preparation, two sorts of peach shell particles were used: one as unmodified peach shell particles (PS) and another one obtained by acid modification, denoted as MPS. Adsorption of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin by PS and MPS was tested in vitro. Crude extracts of mycotoxins, produced at the Department of Microbiology of Bio-ecological Center, Zrenjanin, were used for adsorption experiments. The ability of binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4, which pH value was adjusted to 3.0 and 7.0, respectively. Mass ratio of individual mycotoxin and peach shell samples was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25ºC). After incubation, the extractions of non-adsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantification was done by thin-layer chromatography (TLC).
PB  - Matica Srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - In vitro ocena koštice breskve kao adsorbensa mikotoksina
T1  - In vitro evaluation of the efficacy of peach stones as mycotoxin binders
EP  - 296
IS  - 124
SP  - 287
UR  - conv_169
ER  - 

@article{
author = "Lopičić, Zorica and Bočarov-Stančić, Aleksandra and Stojanović, Mirjana and Milojković, Jelena and Pantić, Vladimir R. and Adamović, Milan",
year = "2013",
abstract = "Rad opisuje in vitro model za procenu sposobnosti koštice breskve (nemodifikovane i modifikovane), pripremljene u Institutu za tehnologiju nuklearnih i drugih mineralnih sirovina u Beogradu, da adsorbuje različite mikotoksine. Koštice breskve su nabavljene od 'Vino Župe' iz Aleksandrovca, kompanije gde su odložene kao otpadni materijal njihove Fabrike sokova. Nakon odgovarajuće pripreme, u ispitivanju su korišćene dve vrste čestica koštice breskve: jedna nemodifikovana (PS) i druga dobijena kiselinskom modifikacijom (MPS). In vitro metodom je testirana adsorpcija šest mikotoksina: aflatoksina B1 (AFL), ohratoksina A (OTA), deoksinivalenola (DON), zearalenona (ZON), di-acetokiscirpenola (DAS) i T-2 toksina. Za eksperimenate adsorpcije korišćeni su sirovi ekstrakti mikotoksina proizvedeni u Odeljenju za mikrobiologiju Bio-ekološkog centra u Zrenjaninu. Sposobnost čestica nemodifikovane i modifikovane koštice breskve za vezivanje mikotoksina je ocenjivana u elektrolitu 0,1 M K2HPO4 čija je pH vrednost podešena na 3,0 odnosno 7,0. Maseni odnos pojedinačnih mikotoksina i uzoraka koštice breskve je bio 1:5000. Eksperimentalne smeše su inkubirane 1 sat na rotacionoj tresilici (185 o/min) na sobnoj temperaturi (22-25ºC). Posle inkubacije, ekstrakcija neadsorbovanih mikotoksina iz filtrata eksperimentalnih smeša su izvršene organskim rastvaračima, a njihova kvantifikacija metodom tankoslojne hromatografije., The paper describes in vitro model for the evaluation of ability of peach shell (unmodified and modified), prepared at the Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, to adsorb different mycotoxins. Peach stones were obtained from 'Vino Župa' Company from Aleksandrovac, where they have been disposed of as by-products from their Juice Factory. After proper preparation, two sorts of peach shell particles were used: one as unmodified peach shell particles (PS) and another one obtained by acid modification, denoted as MPS. Adsorption of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin by PS and MPS was tested in vitro. Crude extracts of mycotoxins, produced at the Department of Microbiology of Bio-ecological Center, Zrenjanin, were used for adsorption experiments. The ability of binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4, which pH value was adjusted to 3.0 and 7.0, respectively. Mass ratio of individual mycotoxin and peach shell samples was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25ºC). After incubation, the extractions of non-adsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantification was done by thin-layer chromatography (TLC).",
publisher = "Matica Srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "In vitro ocena koštice breskve kao adsorbensa mikotoksina, In vitro evaluation of the efficacy of peach stones as mycotoxin binders",
pages = "296-287",
number = "124",
url = "conv_169"
}

Lopičić, Z., Bočarov-Stančić, A., Stojanović, M., Milojković, J., Pantić, V. R.,& Adamović, M.. (2013). In vitro ocena koštice breskve kao adsorbensa mikotoksina. in Zbornik Matice srpske za prirodne nauke
Matica Srpska, Novi Sad.(124), 287-296.
conv_169

Lopičić Z, Bočarov-Stančić A, Stojanović M, Milojković J, Pantić VR, Adamović M. In vitro ocena koštice breskve kao adsorbensa mikotoksina. in Zbornik Matice srpske za prirodne nauke. 2013;(124):287-296.
conv_169 .

Lopičić, Zorica, Bočarov-Stančić, Aleksandra, Stojanović, Mirjana, Milojković, Jelena, Pantić, Vladimir R., Adamović, Milan, "In vitro ocena koštice breskve kao adsorbensa mikotoksina" in Zbornik Matice srpske za prirodne nauke, no. 124 (2013):287-296,
conv_169 .

TY  - CONF
AU  - Bočarov-Stančić, A.S.
AU  - Lopičić, Zorica
AU  - Milojković, Jelena
AU  - Adamović, Milan
AU  - Salma, N.M.
AU  - Pantić, Vladimir R.
PY  - 2012
UR  - https://ritnms.itnms.ac.rs/handle/123456789/238
AB  - The paper describes in vitro model for evaluation of ability of two adsorbents of plant origin, to adsorb different mycotoxins. Fresh harvested tissues of submerged aquatic plant Myriophyllum spicatum, obtained from Sava lake - Belgrade, was washed with HCl solution (3%) and then with distilled water. Plant material was afterwards dried for few days at room temperature, then for 6 hours at 50°C, crushed and sifted. Peach stones, obtained from "Vino Župa" - Aleksandrovac, were separated from soft fruit residues, washed, dried at room temperature, crushed and separated from kernels. The peach stones were further used as milled shell fractions. A total of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin were tested in vitro. For adsorption experiments crude extracts of mycotoxins were used. The ability for binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4 (pH 3.0 and 6.9, respectively). Mass ratio of individual mycotoxin and natural mineral adsorbent was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25°C). The extractions of nonadsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantifications were done by thin-layer chromatography. M. spicatum bound more (94.7-96.0%) of applied AFL B1 than peach stones (73.3-80.0%). In the case of OTA higher adsorption indexes were observed by peach stones (50.0-66.7%). Binding of DON has been observed only by peach stones. Its adsorption index varied from 25.0 to 50.0% depending on the pH of the used electrolyte. M. spicatum adsorbed more ZON at pH 6.9 while peach stones adsorbed more ZON at pH 3.0. In the case of type A trichothecenes (DAS and T-2 toxin) adsorbents of plant origin bound only T-2 toxin. The amount of adsorbed T-2 toxin ranged from 16.7 (pH 3.0) to 33.33% (pH 6.9).
PB  - University of Novi Sad, Faculty of Technology
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - The efficiency of in vitro adsorption of mycotoxins by adsorbents of plant origin
EP  - 1136
SP  - 1131
UR  - conv_989
ER  - 

@conference{
author = "Bočarov-Stančić, A.S. and Lopičić, Zorica and Milojković, Jelena and Adamović, Milan and Salma, N.M. and Pantić, Vladimir R.",
year = "2012",
abstract = "The paper describes in vitro model for evaluation of ability of two adsorbents of plant origin, to adsorb different mycotoxins. Fresh harvested tissues of submerged aquatic plant Myriophyllum spicatum, obtained from Sava lake - Belgrade, was washed with HCl solution (3%) and then with distilled water. Plant material was afterwards dried for few days at room temperature, then for 6 hours at 50°C, crushed and sifted. Peach stones, obtained from "Vino Župa" - Aleksandrovac, were separated from soft fruit residues, washed, dried at room temperature, crushed and separated from kernels. The peach stones were further used as milled shell fractions. A total of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin were tested in vitro. For adsorption experiments crude extracts of mycotoxins were used. The ability for binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4 (pH 3.0 and 6.9, respectively). Mass ratio of individual mycotoxin and natural mineral adsorbent was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25°C). The extractions of nonadsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantifications were done by thin-layer chromatography. M. spicatum bound more (94.7-96.0%) of applied AFL B1 than peach stones (73.3-80.0%). In the case of OTA higher adsorption indexes were observed by peach stones (50.0-66.7%). Binding of DON has been observed only by peach stones. Its adsorption index varied from 25.0 to 50.0% depending on the pH of the used electrolyte. M. spicatum adsorbed more ZON at pH 6.9 while peach stones adsorbed more ZON at pH 3.0. In the case of type A trichothecenes (DAS and T-2 toxin) adsorbents of plant origin bound only T-2 toxin. The amount of adsorbed T-2 toxin ranged from 16.7 (pH 3.0) to 33.33% (pH 6.9).",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "The efficiency of in vitro adsorption of mycotoxins by adsorbents of plant origin",
pages = "1136-1131",
url = "conv_989"
}

Bočarov-Stančić, A.S., Lopičić, Z., Milojković, J., Adamović, M., Salma, N.M.,& Pantić, V. R.. (2012). The efficiency of in vitro adsorption of mycotoxins by adsorbents of plant origin. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
University of Novi Sad, Faculty of Technology., 1131-1136.
conv_989

Bočarov-Stančić A, Lopičić Z, Milojković J, Adamović M, Salma N, Pantić VR. The efficiency of in vitro adsorption of mycotoxins by adsorbents of plant origin. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:1131-1136.
conv_989 .

Bočarov-Stančić, A.S., Lopičić, Zorica, Milojković, Jelena, Adamović, Milan, Salma, N.M., Pantić, Vladimir R., "The efficiency of in vitro adsorption of mycotoxins by adsorbents of plant origin" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):1131-1136,
conv_989 .