@conference{
author = "Antanasković, Anja and Ilić, Nevena and Milivojević, Milan and Dimitrijević-Branković, Suzana and Lopičić, Zorica and Vuković, Nikola",
year = "2024",
abstract = "The rise in global food production and consumption has resulted in waste biomass accumulation at the local landfills, which represents economic and environmental challenges. This biomass, rich with lignocellulosic components, is recognized as valuable resources that align with the principles of zero waste and circular economy. Recently, these materials have garnered growing interest as a promising renewable resource with multifunctional properties as energy fuel, adsorbents, green chemical sources, etc. In this study, waste lignocellulosic biomasses were used as a support material for enzyme immobilization, attributed to their accessibility, surface functional groups, and porosity. In this study, food waste (peach stone (PS), sour cherry stone (CS) and plum stone (PLS)) were thermally treated (pyrolysis) to obtain biochar, material rich in carbon content, with high specific surface area, porosity and significant presence of aromatic functional groups, appropriate for organic materials binding. Biochars were further chemically modified (acid treatment) to produce: peach stone biochar (PSB), sour cherry stone biochar (CSB), and plum stone biochar (PLSB) for potential application for laccase immobilization. All biochars were characterized by pH suspension (pHsus), Fourier transform infra-red (FTIR-ATR) technique and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM–EDX). The successful immobilization of commercial laccase from T. versicolor (0.274 U/ml) on obtained biochars was performed by the adsorption process. The findings showed that the optimal parameters for laccase immobilization were following: pH=5, temperature 40°C, and contact time 24 hours. Immobilization efficiency (IE) and residual activity (RA) were determined for all types of biochars. Cherry stone biochar showed the highest IE (91%) and RA (77%), compared to PSB (IE of 36%, RA of 16%) and PLSB (IE of 86%, RA of 44%). These findings are in accordance with SEM results, confirming that CSB has highly developed porous structure with deepest pores and cracks. This study demonstrates the potential of utilizing modified food waste biomass, particularly cherry stone, as an effective and sustainable enzyme carrier, with future research aimed at exploring its application for the removal of various contaminants from wastewater, contributing to reducing the environmental risks.",
publisher = "Bor : University of Belgrade, Technical Faculty in Bor",
journal = "The 31st International Conference Ecological Truth & Environmental Research – EcoTER'24",
title = "Enzyme immobilization on modified biomass: optimization and characterization",
pages = "728-727"
}
