TY  - CONF
AU  - Stevanović, Marija
AU  - Jovanović, Aleksandar
AU  - Đorđević, Tijana
AU  - Tomašević, Anđelka
AU  - Marinković, Aleksandar
PY  - 2023
UR  - https://ritnms.itnms.ac.rs/handle/123456789/660
AB  - Worldwide consumption of pesticide active ingredients reaches 2.7 million tons per
year [1]. After being released into the environment significant portion of applied pesticides
ends up in surface water bodies affecting nontarget organisms. Due to high environmental
pollution, improvement in technological practices for water treatment has been a demand,
and advanced oxidation processes stand out as a prominent method. In this study,
photocatalytic degradation of a fungicide difenoconazole was investigated and residual
toxicity was assessed using zebrafish embryos (Danio rerio).
Difenoconazole has been subjected to photocatalytic degradation in presence of the
TiO2 Degussa P-25 catalyst and artificial illumination (Osram Ultra Vitalux 300 W).
Influence of different anions (carbonate, sulphate and nitrate), concentration of pesticide and
mass of catalyst, on degradation process has been assessed. Decrease in absorbance
(Shimadzu 1800 UV spectrophotometer) served as an indicator of the pesticide
concentration in the reaction system. The environmental acceptability of the specified
degradation process was assessed for optimal circumstances of total photodegradation.
Residual toxicity of degradation product was assessed on the basis of toxicity comparison
of initial, partly and completely degraded samples, according to OECD 210 [2].
Embryotoxicity assays were performed in triplicate and were terminated 120 hours post
fertilization (hpf).
According to the results of assays engaged in assessment of the influence of the ions
on photocatalytic processes, it was found that all ions have comparable catalytic effects on
the kinetics of the difenoconazole degradation process. The presence of sulphates and
carbonates had the greatest catalytic effect, while the nitrate ions showed the lowest catalytic
capacity (Fig. 1). The most suitable conditions for photodegradation were obtained by using
0.2 g/l of TiO2 and 5 mg/l of difenoconazole solution at atmospheric conditions. The
embryotoxicity assays followed the analytical examination. Comparison of results obtained
in embryotoxicity assays of the initial solution, partly and completely degraded samples
confirmed suitability of applied degradation method. Significant decrease in toxicity,
compared to the initial solution, was registered in both degraded samples. Based on
cumulative mortality (120 hpf), medium lethal concentration (LC50) of difenoconazole is 1.4
mg/l, while the same parameter could not be determined in partly and completely degraded
samples, due to the lack of mortality (Fig. 2).
According to the obtained results it can be concluded that engaged photocatalytic
degradation process is suitable for pesticide contamination water management.
PB  - Belgrade : Serbian Chemical Society
C3  - 9th Symposium Chemistry and Environmental Protection EnviroChem2023
T1  - Photocatalytic degradation of a fungicide difenoconazole and residual toxicity of its degradation products
EP  - 182
SP  - 181
ER  - 

@conference{
author = "Stevanović, Marija and Jovanović, Aleksandar and Đorđević, Tijana and Tomašević, Anđelka and Marinković, Aleksandar",
year = "2023",
abstract = "Worldwide consumption of pesticide active ingredients reaches 2.7 million tons per
year [1]. After being released into the environment significant portion of applied pesticides
ends up in surface water bodies affecting nontarget organisms. Due to high environmental
pollution, improvement in technological practices for water treatment has been a demand,
and advanced oxidation processes stand out as a prominent method. In this study,
photocatalytic degradation of a fungicide difenoconazole was investigated and residual
toxicity was assessed using zebrafish embryos (Danio rerio).
Difenoconazole has been subjected to photocatalytic degradation in presence of the
TiO2 Degussa P-25 catalyst and artificial illumination (Osram Ultra Vitalux 300 W).
Influence of different anions (carbonate, sulphate and nitrate), concentration of pesticide and
mass of catalyst, on degradation process has been assessed. Decrease in absorbance
(Shimadzu 1800 UV spectrophotometer) served as an indicator of the pesticide
concentration in the reaction system. The environmental acceptability of the specified
degradation process was assessed for optimal circumstances of total photodegradation.
Residual toxicity of degradation product was assessed on the basis of toxicity comparison
of initial, partly and completely degraded samples, according to OECD 210 [2].
Embryotoxicity assays were performed in triplicate and were terminated 120 hours post
fertilization (hpf).
According to the results of assays engaged in assessment of the influence of the ions
on photocatalytic processes, it was found that all ions have comparable catalytic effects on
the kinetics of the difenoconazole degradation process. The presence of sulphates and
carbonates had the greatest catalytic effect, while the nitrate ions showed the lowest catalytic
capacity (Fig. 1). The most suitable conditions for photodegradation were obtained by using
0.2 g/l of TiO2 and 5 mg/l of difenoconazole solution at atmospheric conditions. The
embryotoxicity assays followed the analytical examination. Comparison of results obtained
in embryotoxicity assays of the initial solution, partly and completely degraded samples
confirmed suitability of applied degradation method. Significant decrease in toxicity,
compared to the initial solution, was registered in both degraded samples. Based on
cumulative mortality (120 hpf), medium lethal concentration (LC50) of difenoconazole is 1.4
mg/l, while the same parameter could not be determined in partly and completely degraded
samples, due to the lack of mortality (Fig. 2).
According to the obtained results it can be concluded that engaged photocatalytic
degradation process is suitable for pesticide contamination water management.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "9th Symposium Chemistry and Environmental Protection EnviroChem2023",
title = "Photocatalytic degradation of a fungicide difenoconazole and residual toxicity of its degradation products",
pages = "182-181"
}

Stevanović, M., Jovanović, A., Đorđević, T., Tomašević, A.,& Marinković, A.. (2023). Photocatalytic degradation of a fungicide difenoconazole and residual toxicity of its degradation products. in 9th Symposium Chemistry and Environmental Protection EnviroChem2023
Belgrade : Serbian Chemical Society., 181-182.

Stevanović M, Jovanović A, Đorđević T, Tomašević A, Marinković A. Photocatalytic degradation of a fungicide difenoconazole and residual toxicity of its degradation products. in 9th Symposium Chemistry and Environmental Protection EnviroChem2023. 2023;:181-182..

Stevanović, Marija, Jovanović, Aleksandar, Đorđević, Tijana, Tomašević, Anđelka, Marinković, Aleksandar, "Photocatalytic degradation of a fungicide difenoconazole and residual toxicity of its degradation products" in 9th Symposium Chemistry and Environmental Protection EnviroChem2023 (2023):181-182.

TY  - CONF
AU  - Stevanović, Marija
AU  - Jovanović, Aleksandar
AU  - Đolić, Maja
AU  - Veličković, Zlate
AU  - Čutović, Natalija
AU  - Tomašević, Anđelka
AU  - Marinković, Aleksandar
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/663
AB  - Pesticides are substances designed to protect plants
from various types of diseases and pests [1]under UV irradiation
is studied using synthesized Zinc oxide (ZnO.
Synthetic organic pesticides, in addition to the high
efficiency, have led to frequent adverse environmental
impact, as a consequence of their high accumulation
and toxicity. Due to the increased pollution of water
with mixture pesticides, it is necessary to use different
processes for their removal and degradation. Therefore,
oxidative processes have been developed, commonly
named as Advanced Oxidation Processes (AOPs).
Among them, special attention was attributed to photocatalysis,
as a process that enables the degradation
of difficult-to-decompose organic molecules under the
action of UV radiation in the presence of catalysts [2].
In this study the photocatalytic degradation of thiophanate-
methyl (TM) in the presence of the TiO2 Degussa
P-25 catalyst was investigated. Different experimental
conditions were varied, such as the concentration of
the pesticide solution, the mass of the catalyst and the
influence of the anions (chloride, sulphate, nitrate, etc.).
The pesticide concentration in the reaction system was
monitored based on the decrease in system absorbance
using a Shimadzu 1800 UV spectrophotometer. For optimized
conditions of complete photodegradation, the
environmental acceptability of the defined degradation
process was examined. The toxic effect of the TM solution
before and after degradation was examined using
the embryotoxicity test with Danio rerio, in order to
prove the reduction of toxicity and the success of the
degradation process [3]which raises the issue of potential
influence of different formulation types on herbicide
toxicity. The present study evaluated the toxicity and
teratogenic effects of the active ingredient clomazone
and its two formulations (Rampa® EC and GAT Cenit
36 CS, both containing 360 g a.i./l of clomazone.
Comparing the obtained results of the influence of
ions on the processes of photocatalysis, it was noticed
that all ions have catalytic effects on the kinetics of the
degradation process of TM. The presence of sulphates
and carbonates had the greatest catalytic effect, while
hydrogen phosphates and bicarbonates showed the lowest
catalytic capacity.
The optimal experimental conditions were obtained
using 0,2 g/L of TiO2 and 5 mg/L of TM solution. In
addition, the embryotoxicity test followed the analytical
examination. Comparison of results obtained in embryotoxicity
assay testing of the initial solution, partly
and completely degraded samples confirmed suitability
of applied degradation method. Increase in toxicity,
compared to the initial solution, was registered in partly
degraded sample. This observation can be attributed to
increase in concentration of carbendazim (TM metabolite)
more toxic than parent substance. Finally, completely
degraded sample caused no mortality or adverse
effects in D. rerio embryos after 120 h exposure. Toxicity
of samples, in decreasing order is half degraded >
initial >completely degraded sample.
Based on the obtained results it can be concluded
that used photocatalytic degradation process can be successfully
applied in pesticide contaminated water management.
PB  - Belgrade : Serbian Chemical Society
C3  - 21th European Meeting on Environmental Chemistry EMEC 21
T1  - The Embryotoxic Potential and Photocatalytic Degradation of Thiophanate-Methyl
EP  - 42
SP  - 42
ER  - 

@conference{
author = "Stevanović, Marija and Jovanović, Aleksandar and Đolić, Maja and Veličković, Zlate and Čutović, Natalija and Tomašević, Anđelka and Marinković, Aleksandar",
year = "2021",
abstract = "Pesticides are substances designed to protect plants
from various types of diseases and pests [1]under UV irradiation
is studied using synthesized Zinc oxide (ZnO.
Synthetic organic pesticides, in addition to the high
efficiency, have led to frequent adverse environmental
impact, as a consequence of their high accumulation
and toxicity. Due to the increased pollution of water
with mixture pesticides, it is necessary to use different
processes for their removal and degradation. Therefore,
oxidative processes have been developed, commonly
named as Advanced Oxidation Processes (AOPs).
Among them, special attention was attributed to photocatalysis,
as a process that enables the degradation
of difficult-to-decompose organic molecules under the
action of UV radiation in the presence of catalysts [2].
In this study the photocatalytic degradation of thiophanate-
methyl (TM) in the presence of the TiO2 Degussa
P-25 catalyst was investigated. Different experimental
conditions were varied, such as the concentration of
the pesticide solution, the mass of the catalyst and the
influence of the anions (chloride, sulphate, nitrate, etc.).
The pesticide concentration in the reaction system was
monitored based on the decrease in system absorbance
using a Shimadzu 1800 UV spectrophotometer. For optimized
conditions of complete photodegradation, the
environmental acceptability of the defined degradation
process was examined. The toxic effect of the TM solution
before and after degradation was examined using
the embryotoxicity test with Danio rerio, in order to
prove the reduction of toxicity and the success of the
degradation process [3]which raises the issue of potential
influence of different formulation types on herbicide
toxicity. The present study evaluated the toxicity and
teratogenic effects of the active ingredient clomazone
and its two formulations (Rampa® EC and GAT Cenit
36 CS, both containing 360 g a.i./l of clomazone.
Comparing the obtained results of the influence of
ions on the processes of photocatalysis, it was noticed
that all ions have catalytic effects on the kinetics of the
degradation process of TM. The presence of sulphates
and carbonates had the greatest catalytic effect, while
hydrogen phosphates and bicarbonates showed the lowest
catalytic capacity.
The optimal experimental conditions were obtained
using 0,2 g/L of TiO2 and 5 mg/L of TM solution. In
addition, the embryotoxicity test followed the analytical
examination. Comparison of results obtained in embryotoxicity
assay testing of the initial solution, partly
and completely degraded samples confirmed suitability
of applied degradation method. Increase in toxicity,
compared to the initial solution, was registered in partly
degraded sample. This observation can be attributed to
increase in concentration of carbendazim (TM metabolite)
more toxic than parent substance. Finally, completely
degraded sample caused no mortality or adverse
effects in D. rerio embryos after 120 h exposure. Toxicity
of samples, in decreasing order is half degraded >
initial >completely degraded sample.
Based on the obtained results it can be concluded
that used photocatalytic degradation process can be successfully
applied in pesticide contaminated water management.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "21th European Meeting on Environmental Chemistry EMEC 21",
title = "The Embryotoxic Potential and Photocatalytic Degradation of Thiophanate-Methyl",
pages = "42-42"
}

Stevanović, M., Jovanović, A., Đolić, M., Veličković, Z., Čutović, N., Tomašević, A.,& Marinković, A.. (2021). The Embryotoxic Potential and Photocatalytic Degradation of Thiophanate-Methyl. in 21th European Meeting on Environmental Chemistry EMEC 21
Belgrade : Serbian Chemical Society., 42-42.

Stevanović M, Jovanović A, Đolić M, Veličković Z, Čutović N, Tomašević A, Marinković A. The Embryotoxic Potential and Photocatalytic Degradation of Thiophanate-Methyl. in 21th European Meeting on Environmental Chemistry EMEC 21. 2021;:42-42..

Stevanović, Marija, Jovanović, Aleksandar, Đolić, Maja, Veličković, Zlate, Čutović, Natalija, Tomašević, Anđelka, Marinković, Aleksandar, "The Embryotoxic Potential and Photocatalytic Degradation of Thiophanate-Methyl" in 21th European Meeting on Environmental Chemistry EMEC 21 (2021):42-42.

TY  - CONF
AU  - Tomašević, Anđelka
AU  - Jovanović, Aleksandar
AU  - Bošnjaković, Jovana
AU  - Stevanović, Marija
AU  - Rusmirović, Jelena
AU  - Marinković, Aleksandar
PY  - 2021
UR  - https://ritnms.itnms.ac.rs/handle/123456789/662
AB  - With the development of the industry and growth of
the population, there is an increasing amount of waste,
which, due to inadequate treatment, pollutes water. The
group of the most dangerous pollutants present in water
includes heavy metals, such as As, Cd, Pb, Ni, Hg, Cr,
etc. [1]. Heavy metal ions are highly toxic and not biodegradable,
but are prone to accumulation in the body in
certain tissues and organs [2].
In recent years, natural materials, originating from
waste or renewable sources, have been increasingly
used as adsorbents in the removal of heavy metal ions
from water, due to their low cost, high prevalence and
beneficial impact on the environment [3]. Lignin, cellulose
and hemicellulose are the main polymers of wood
biomass [4]. Lignin is represented as a by-product in the
paper and pulp industry [5]. Chemical modification of
lignin was performed using acrylate derivatives (L-AC).
Modified lignin microspheres (LMS) were synthesized
by inverse suspension copolymerization using L-AC,
trimethylolpropane triacrylate (TMPTA) and methacryl
functionalized magnetite modified with MEMO silane
or with methacryloyl chloride (MACM1 or MACM2).
The procedure of inverse emulsion-suspension copolymerization
developed by Popović et al. [6] was used. In
a summary, disodium laureth sulfosuccinate (surfactant)
was stirred in water solution for 30 min at 80 °C. Afterwards,
TMPTA, L-MAC, MACM1 or MACM2 and the
initiator AIBN (1 wt. %) were added, followed by the
mixture of pore-forming solvents (tetradecanol and toluene),
stirred for 18 h at the same elevated temperature.
LMS microspheres were characterized by zero
charge point determination, FT-IR and SEM. The efficiency
of pollutants (chromium(VI) and arsenic(V)
ions) removal was analysed in terms of varying the
experimental conditions: the mass of adsorbent, the pH
of solution, the temperature of reaction and the contact
time. The best sorption was observed for the pH between
5.0 and 7.0. Synthesized bio-adsorbents showed
high efficiency, with capacities of 35.5 and 54.0 mg g-1
for the LMS adsorbents loaded with magnetite modified
using methacyl functionalized silane (LMS-1) or
methacryloyl chloride (LMS-2), respectively, obtained
according to Freundlich isothermal model. Adsorption
kinetics are described according to a pseudo-second
order model. Based on the obtained results, both adsorbents
showed excellent adsorption abilities.
Thermodynamic parameters, including the Gibbs
free energy (ΔGΘ), enthalpy (ΔHΘ) and entropy (ΔSΘ),
proved that adsorption is viable, spontaneous and endothermic
process (LMS-1) and exothermic process
(LMS-2) at temperatures between 25 and 45 °C.
PB  - Belgrade : Serbian Chemical Society
C3  - 21th European Meeting on Environmental Chemistry EMEC 21
T1  - Removal of Chromium(VI) and Arsenic(V) from Water Solution Using Modified Lignin Microspheres
EP  - 115
SP  - 115
ER  - 

@conference{
author = "Tomašević, Anđelka and Jovanović, Aleksandar and Bošnjaković, Jovana and Stevanović, Marija and Rusmirović, Jelena and Marinković, Aleksandar",
year = "2021",
abstract = "With the development of the industry and growth of
the population, there is an increasing amount of waste,
which, due to inadequate treatment, pollutes water. The
group of the most dangerous pollutants present in water
includes heavy metals, such as As, Cd, Pb, Ni, Hg, Cr,
etc. [1]. Heavy metal ions are highly toxic and not biodegradable,
but are prone to accumulation in the body in
certain tissues and organs [2].
In recent years, natural materials, originating from
waste or renewable sources, have been increasingly
used as adsorbents in the removal of heavy metal ions
from water, due to their low cost, high prevalence and
beneficial impact on the environment [3]. Lignin, cellulose
and hemicellulose are the main polymers of wood
biomass [4]. Lignin is represented as a by-product in the
paper and pulp industry [5]. Chemical modification of
lignin was performed using acrylate derivatives (L-AC).
Modified lignin microspheres (LMS) were synthesized
by inverse suspension copolymerization using L-AC,
trimethylolpropane triacrylate (TMPTA) and methacryl
functionalized magnetite modified with MEMO silane
or with methacryloyl chloride (MACM1 or MACM2).
The procedure of inverse emulsion-suspension copolymerization
developed by Popović et al. [6] was used. In
a summary, disodium laureth sulfosuccinate (surfactant)
was stirred in water solution for 30 min at 80 °C. Afterwards,
TMPTA, L-MAC, MACM1 or MACM2 and the
initiator AIBN (1 wt. %) were added, followed by the
mixture of pore-forming solvents (tetradecanol and toluene),
stirred for 18 h at the same elevated temperature.
LMS microspheres were characterized by zero
charge point determination, FT-IR and SEM. The efficiency
of pollutants (chromium(VI) and arsenic(V)
ions) removal was analysed in terms of varying the
experimental conditions: the mass of adsorbent, the pH
of solution, the temperature of reaction and the contact
time. The best sorption was observed for the pH between
5.0 and 7.0. Synthesized bio-adsorbents showed
high efficiency, with capacities of 35.5 and 54.0 mg g-1
for the LMS adsorbents loaded with magnetite modified
using methacyl functionalized silane (LMS-1) or
methacryloyl chloride (LMS-2), respectively, obtained
according to Freundlich isothermal model. Adsorption
kinetics are described according to a pseudo-second
order model. Based on the obtained results, both adsorbents
showed excellent adsorption abilities.
Thermodynamic parameters, including the Gibbs
free energy (ΔGΘ), enthalpy (ΔHΘ) and entropy (ΔSΘ),
proved that adsorption is viable, spontaneous and endothermic
process (LMS-1) and exothermic process
(LMS-2) at temperatures between 25 and 45 °C.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "21th European Meeting on Environmental Chemistry EMEC 21",
title = "Removal of Chromium(VI) and Arsenic(V) from Water Solution Using Modified Lignin Microspheres",
pages = "115-115"
}

Tomašević, A., Jovanović, A., Bošnjaković, J., Stevanović, M., Rusmirović, J.,& Marinković, A.. (2021). Removal of Chromium(VI) and Arsenic(V) from Water Solution Using Modified Lignin Microspheres. in 21th European Meeting on Environmental Chemistry EMEC 21
Belgrade : Serbian Chemical Society., 115-115.

Tomašević A, Jovanović A, Bošnjaković J, Stevanović M, Rusmirović J, Marinković A. Removal of Chromium(VI) and Arsenic(V) from Water Solution Using Modified Lignin Microspheres. in 21th European Meeting on Environmental Chemistry EMEC 21. 2021;:115-115..

Tomašević, Anđelka, Jovanović, Aleksandar, Bošnjaković, Jovana, Stevanović, Marija, Rusmirović, Jelena, Marinković, Aleksandar, "Removal of Chromium(VI) and Arsenic(V) from Water Solution Using Modified Lignin Microspheres" in 21th European Meeting on Environmental Chemistry EMEC 21 (2021):115-115.