TY  - JOUR
AU  - Campagnollo, Femanda B.
AU  - Franco, Larissa T.
AU  - Rottinghaus, George E.
AU  - Kobashigawa, Estela
AU  - Ledoux, David R.
AU  - Daković, Aleksandra
AU  - Oliveira, Carlos A. F.
PY  - 2015
UR  - https://ritnms.itnms.ac.rs/handle/123456789/339
AB  - In vitro tests were performed to determine the ability of beer fermentation residue (BFR) containing Saccharomyces cerevisiae to bind aflatoxin B-1 (AFB(1)), zearalenone (ZEA), ochratoxin A (OTA) and deoxynivalenol (DON). BFR was obtained from a microbrewery, dried and ground, resulting in approximately 1.0 x 10(10) S. cerevisiae cells g(-1) BFR. Binding assays consisted of suspending BFR (100 mg) in 10 mL of buffer solution (pH 3.0 or 6.5) spiked with AFB(1), ZEA, OTA or DON (2.0 mu g ml(-1) of each mycotoxin), incubation (60 min, 25 degrees C) followed by centrifugation. The supernatants were analyzed by high performance liquid chromatography. BFR had higher binding capacity for ZEA (75.1% and 77.5% at pH 3.0 and 6.5, respectively), when compared with AFB(1), OTA and DON (less than 60% and 40% at pH 3.0 and 6.5, respectively). BFR also produced linear isotherms for ZEA at both pH values, hence indicating a potential application of industrial fermentation by-products containing yeast cells in reducing the bioavailability of ZEA in contaminated feedstuffs. However, in vivo studies are required to prove its efficacy in livestock and poultry.
PB  - Elsevier, Amsterdam
T2  - Food Research International
T1  - In vitro evaluation of the ability of beer fermentation residue containing Saccharomyces cerevisiae to bind mycotoxins
EP  - 648
SP  - 643
VL  - 77
DO  - 10.1016/j.foodres.2015.08.032
UR  - conv_748
ER  - 

@article{
author = "Campagnollo, Femanda B. and Franco, Larissa T. and Rottinghaus, George E. and Kobashigawa, Estela and Ledoux, David R. and Daković, Aleksandra and Oliveira, Carlos A. F.",
year = "2015",
abstract = "In vitro tests were performed to determine the ability of beer fermentation residue (BFR) containing Saccharomyces cerevisiae to bind aflatoxin B-1 (AFB(1)), zearalenone (ZEA), ochratoxin A (OTA) and deoxynivalenol (DON). BFR was obtained from a microbrewery, dried and ground, resulting in approximately 1.0 x 10(10) S. cerevisiae cells g(-1) BFR. Binding assays consisted of suspending BFR (100 mg) in 10 mL of buffer solution (pH 3.0 or 6.5) spiked with AFB(1), ZEA, OTA or DON (2.0 mu g ml(-1) of each mycotoxin), incubation (60 min, 25 degrees C) followed by centrifugation. The supernatants were analyzed by high performance liquid chromatography. BFR had higher binding capacity for ZEA (75.1% and 77.5% at pH 3.0 and 6.5, respectively), when compared with AFB(1), OTA and DON (less than 60% and 40% at pH 3.0 and 6.5, respectively). BFR also produced linear isotherms for ZEA at both pH values, hence indicating a potential application of industrial fermentation by-products containing yeast cells in reducing the bioavailability of ZEA in contaminated feedstuffs. However, in vivo studies are required to prove its efficacy in livestock and poultry.",
publisher = "Elsevier, Amsterdam",
journal = "Food Research International",
title = "In vitro evaluation of the ability of beer fermentation residue containing Saccharomyces cerevisiae to bind mycotoxins",
pages = "648-643",
volume = "77",
doi = "10.1016/j.foodres.2015.08.032",
url = "conv_748"
}

Campagnollo, F. B., Franco, L. T., Rottinghaus, G. E., Kobashigawa, E., Ledoux, D. R., Daković, A.,& Oliveira, C. A. F.. (2015). In vitro evaluation of the ability of beer fermentation residue containing Saccharomyces cerevisiae to bind mycotoxins. in Food Research International
Elsevier, Amsterdam., 77, 643-648.
https://doi.org/10.1016/j.foodres.2015.08.032
conv_748

Campagnollo FB, Franco LT, Rottinghaus GE, Kobashigawa E, Ledoux DR, Daković A, Oliveira CAF. In vitro evaluation of the ability of beer fermentation residue containing Saccharomyces cerevisiae to bind mycotoxins. in Food Research International. 2015;77:643-648.
doi:10.1016/j.foodres.2015.08.032
conv_748 .

Campagnollo, Femanda B., Franco, Larissa T., Rottinghaus, George E., Kobashigawa, Estela, Ledoux, David R., Daković, Aleksandra, Oliveira, Carlos A. F., "In vitro evaluation of the ability of beer fermentation residue containing Saccharomyces cerevisiae to bind mycotoxins" in Food Research International, 77 (2015):643-648,
https://doi.org/10.1016/j.foodres.2015.08.032 .,
conv_748 .

TY  - JOUR
AU  - Neeff, D. V.
AU  - Ledoux, David R.
AU  - Rottinghaus, George E.
AU  - Bermudez, A. J.
AU  - Daković, Aleksandra
AU  - Murarolli, R. A.
AU  - Oliveira, Carlos A. F.
PY  - 2013
UR  - https://ritnms.itnms.ac.rs/handle/123456789/251
AB  - The aim of this study was to determine the binding capacity of a hydrated sodium calcium aluminosilicate (HSCAS) for aflatoxin B-1 (AFB(1)), and the efficacy of the HSCAS to reduce the concentrations of residual AFB(1) and its metabolites in the liver and kidney of broilers fed AFB(1). One hundred 1-d-old male broilers (Ross 708) were maintained in chick batteries and allowed ad libitum access to feed and water. A completely randomized design was used with 5 replicate pens of 5 chicks assigned to each of 4 dietary treatments from hatch to 21 d. Dietary treatments included the following: A) basal diet (BD), with no HSCAS or AFB(1), B) BD supplemented with 0.5% HSCAS only, C) BD supplemented with 2.5 mg of AFB(1)/kg of feed, and D) BD supplemented with 2.5 mg of AFB(1)/kg of feed and 0.5% HSCAS. On d 21, 5 chicks from each treatment were anesthetized with carbon dioxide, killed by cervical dislocation, and samples of liver and kidney were collected for analysis of AFB(1) residues. The percentage of AFB(1) bound for each concentration of adsorbent (100, 10, 1, 0.5, 0.25, and 0.05 mg/10 mL) was 100, 91.1, 81.8, 75.4, 40.1, and 8.8%, respectively. Concentrations of aflatoxin residues (AFB(1), aflatoxicol, aflatoxins B-2 and G(1)) were lower (P  LT  0.05) in livers and kidneys of birds fed AFB(1) plus HSCAS (diet D), when compared with birds fed AFB(1) alone (diet C). However, histopathology data from the in vivo study indicated that HSCAS did not prevent lesions associated with aflatoxicosis. The decrease in the bioavailability of AFB(1) caused by the HSCAS reduced aflatoxin residues in liver and kidney, but not enough to completely prevent the toxic effects of AFB(1) in broilers.
PB  - Oxford Univ Press, Oxford
T2  - Poultry Science
T1  - In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B-1
EP  - 137
IS  - 1
SP  - 131
VL  - 92
DO  - 10.3382/ps.2012-02510
UR  - conv_642
ER  - 

@article{
author = "Neeff, D. V. and Ledoux, David R. and Rottinghaus, George E. and Bermudez, A. J. and Daković, Aleksandra and Murarolli, R. A. and Oliveira, Carlos A. F.",
year = "2013",
abstract = "The aim of this study was to determine the binding capacity of a hydrated sodium calcium aluminosilicate (HSCAS) for aflatoxin B-1 (AFB(1)), and the efficacy of the HSCAS to reduce the concentrations of residual AFB(1) and its metabolites in the liver and kidney of broilers fed AFB(1). One hundred 1-d-old male broilers (Ross 708) were maintained in chick batteries and allowed ad libitum access to feed and water. A completely randomized design was used with 5 replicate pens of 5 chicks assigned to each of 4 dietary treatments from hatch to 21 d. Dietary treatments included the following: A) basal diet (BD), with no HSCAS or AFB(1), B) BD supplemented with 0.5% HSCAS only, C) BD supplemented with 2.5 mg of AFB(1)/kg of feed, and D) BD supplemented with 2.5 mg of AFB(1)/kg of feed and 0.5% HSCAS. On d 21, 5 chicks from each treatment were anesthetized with carbon dioxide, killed by cervical dislocation, and samples of liver and kidney were collected for analysis of AFB(1) residues. The percentage of AFB(1) bound for each concentration of adsorbent (100, 10, 1, 0.5, 0.25, and 0.05 mg/10 mL) was 100, 91.1, 81.8, 75.4, 40.1, and 8.8%, respectively. Concentrations of aflatoxin residues (AFB(1), aflatoxicol, aflatoxins B-2 and G(1)) were lower (P  LT  0.05) in livers and kidneys of birds fed AFB(1) plus HSCAS (diet D), when compared with birds fed AFB(1) alone (diet C). However, histopathology data from the in vivo study indicated that HSCAS did not prevent lesions associated with aflatoxicosis. The decrease in the bioavailability of AFB(1) caused by the HSCAS reduced aflatoxin residues in liver and kidney, but not enough to completely prevent the toxic effects of AFB(1) in broilers.",
publisher = "Oxford Univ Press, Oxford",
journal = "Poultry Science",
title = "In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B-1",
pages = "137-131",
number = "1",
volume = "92",
doi = "10.3382/ps.2012-02510",
url = "conv_642"
}

Neeff, D. V., Ledoux, D. R., Rottinghaus, G. E., Bermudez, A. J., Daković, A., Murarolli, R. A.,& Oliveira, C. A. F.. (2013). In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B-1. in Poultry Science
Oxford Univ Press, Oxford., 92(1), 131-137.
https://doi.org/10.3382/ps.2012-02510
conv_642

Neeff DV, Ledoux DR, Rottinghaus GE, Bermudez AJ, Daković A, Murarolli RA, Oliveira CAF. In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B-1. in Poultry Science. 2013;92(1):131-137.
doi:10.3382/ps.2012-02510
conv_642 .

Neeff, D. V., Ledoux, David R., Rottinghaus, George E., Bermudez, A. J., Daković, Aleksandra, Murarolli, R. A., Oliveira, Carlos A. F., "In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B-1" in Poultry Science, 92, no. 1 (2013):131-137,
https://doi.org/10.3382/ps.2012-02510 .,
conv_642 .