Electrochemically synthesized biocomposite poly(vinyl alcohol)/chitosan-based coatings for medical applications
2024
Преузимање 🢃
Аутори
Janković, AnaĐošić, Marija
Stevanović, Milena
Kojić, Vesna
Vukašinović-Sekulić, Maja
Mišković-Stanković, Vesna
Остала ауторства
Dimkić, IvicaKekić, Dušan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Osteoarticular implants are designed to facilitate
full recovery of lost function and ensure effective
fixation of the implant. However, challenges
may arise, resulting in implant failure, primarily
attributed to infections at the implantation site
and aseptic loosening. Thus, the surface of an
implant must be altered to simultaneously offer
osteoinductive and antibacterial properties.
Synthetic hydroxyapatite (HAP) is frequently
employed to modify metallic implant surfaces
or serve as a bone filler material. To enhance
its biocompatibility, HAP-based polymer composites
were developed. Among most popular
synthetic polymers, poly(vinyl alcohol) (PVA), is
widely used because of its similar structure to
the native extracellular matrix, along with chitosan
(CS), a natural cationic polysaccharide, that
shows biocompatibility, biodegradability and
antimicrobial action, and could act as a carrier
of antibacterial agents. The antibiotic of choice
was Gentamicin (Gent), a wa...ter-soluble aminoglycoside,
with very potent antibacterial activity
for the treatment of wide range of infections,
caused by Gram-negative and Gram-positive
bacteria. The original approach of the presented
research is the single-step electrophoretic deposition
(EPD) on Ti plates of thus prepared biocomposite
that would allow for on-site release of the
drug. Obtained hydroxyapatite/chitosan/poly(vinyl
alcohol/gentamicin (HAP/PVA/CS/Gent) biocomposite
coating exhibited strong antibacterial
effect against E. coli and S. aureus. Gentamicin release
study indicated “burst” release in the first 48
h, with ~ 30% of total gentamicin released from
the HAP/PVA/CS/Gent coating which is beneficial
for the blockage of biofilm formation, followed
by slow and steady release in the later period. Cytotoxic
effect of HAP/PVA/CS/Gent coating was
not pronounced in investigated MRC-5 and L929
cell lines. Also, in MRC-5 fibroblast cells, alkaline
phosphatase levels doubled when in contact
with HAP/PVA/CS/Gent, indicating good osteogenic
properties. The overall construct on the
substrate in such a form would be well-advanced
arrangement for future medical device improvement
of skeletal implants.
Кључне речи:
antibacterial / gentamicim release / poly(vinyl alcohol) / chitosan / electrophoretic depositionИзвор:
XIII SERBIAN MICROBIOLOGY CONGRESS with international participation, MIKROMED REGIO 5, UMS Series 24: „FROM BIOTECHNOLOGY TO HUMAN AND PLANETARY HEALTH", 2024, 182-182Издавач:
- Belgrade : Serbian Society for Microbiology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200023 (Институт за технологију нуклеарних и других минералних сировина - ИТНМС, Београд) (RS-MESTD-inst-2020-200023)
- European Union’s Horizon 2020 research and innovation program under grant agreement No. 952033.
- The Ministry of Science, Technological Development and Innovation, Republic of Serbia, (Contract No. 337-00-110/2023-05/13)
Институција/група
Institut za tehnologiju nuklearnih i drugih mineralnih sirovinaTY - CONF AU - Janković, Ana AU - Đošić, Marija AU - Stevanović, Milena AU - Kojić, Vesna AU - Vukašinović-Sekulić, Maja AU - Mišković-Stanković, Vesna PY - 2024 UR - https://ritnms.itnms.ac.rs/handle/123456789/1271 AB - Osteoarticular implants are designed to facilitate full recovery of lost function and ensure effective fixation of the implant. However, challenges may arise, resulting in implant failure, primarily attributed to infections at the implantation site and aseptic loosening. Thus, the surface of an implant must be altered to simultaneously offer osteoinductive and antibacterial properties. Synthetic hydroxyapatite (HAP) is frequently employed to modify metallic implant surfaces or serve as a bone filler material. To enhance its biocompatibility, HAP-based polymer composites were developed. Among most popular synthetic polymers, poly(vinyl alcohol) (PVA), is widely used because of its similar structure to the native extracellular matrix, along with chitosan (CS), a natural cationic polysaccharide, that shows biocompatibility, biodegradability and antimicrobial action, and could act as a carrier of antibacterial agents. The antibiotic of choice was Gentamicin (Gent), a water-soluble aminoglycoside, with very potent antibacterial activity for the treatment of wide range of infections, caused by Gram-negative and Gram-positive bacteria. The original approach of the presented research is the single-step electrophoretic deposition (EPD) on Ti plates of thus prepared biocomposite that would allow for on-site release of the drug. Obtained hydroxyapatite/chitosan/poly(vinyl alcohol/gentamicin (HAP/PVA/CS/Gent) biocomposite coating exhibited strong antibacterial effect against E. coli and S. aureus. Gentamicin release study indicated “burst” release in the first 48 h, with ~ 30% of total gentamicin released from the HAP/PVA/CS/Gent coating which is beneficial for the blockage of biofilm formation, followed by slow and steady release in the later period. Cytotoxic effect of HAP/PVA/CS/Gent coating was not pronounced in investigated MRC-5 and L929 cell lines. Also, in MRC-5 fibroblast cells, alkaline phosphatase levels doubled when in contact with HAP/PVA/CS/Gent, indicating good osteogenic properties. The overall construct on the substrate in such a form would be well-advanced arrangement for future medical device improvement of skeletal implants. PB - Belgrade : Serbian Society for Microbiology C3 - XIII SERBIAN MICROBIOLOGY CONGRESS with international participation, MIKROMED REGIO 5, UMS Series 24: „FROM BIOTECHNOLOGY TO HUMAN AND PLANETARY HEALTH" T1 - Electrochemically synthesized biocomposite poly(vinyl alcohol)/chitosan-based coatings for medical applications EP - 182 SP - 182 ER -
@conference{ author = "Janković, Ana and Đošić, Marija and Stevanović, Milena and Kojić, Vesna and Vukašinović-Sekulić, Maja and Mišković-Stanković, Vesna", year = "2024", abstract = "Osteoarticular implants are designed to facilitate full recovery of lost function and ensure effective fixation of the implant. However, challenges may arise, resulting in implant failure, primarily attributed to infections at the implantation site and aseptic loosening. Thus, the surface of an implant must be altered to simultaneously offer osteoinductive and antibacterial properties. Synthetic hydroxyapatite (HAP) is frequently employed to modify metallic implant surfaces or serve as a bone filler material. To enhance its biocompatibility, HAP-based polymer composites were developed. Among most popular synthetic polymers, poly(vinyl alcohol) (PVA), is widely used because of its similar structure to the native extracellular matrix, along with chitosan (CS), a natural cationic polysaccharide, that shows biocompatibility, biodegradability and antimicrobial action, and could act as a carrier of antibacterial agents. The antibiotic of choice was Gentamicin (Gent), a water-soluble aminoglycoside, with very potent antibacterial activity for the treatment of wide range of infections, caused by Gram-negative and Gram-positive bacteria. The original approach of the presented research is the single-step electrophoretic deposition (EPD) on Ti plates of thus prepared biocomposite that would allow for on-site release of the drug. Obtained hydroxyapatite/chitosan/poly(vinyl alcohol/gentamicin (HAP/PVA/CS/Gent) biocomposite coating exhibited strong antibacterial effect against E. coli and S. aureus. Gentamicin release study indicated “burst” release in the first 48 h, with ~ 30% of total gentamicin released from the HAP/PVA/CS/Gent coating which is beneficial for the blockage of biofilm formation, followed by slow and steady release in the later period. Cytotoxic effect of HAP/PVA/CS/Gent coating was not pronounced in investigated MRC-5 and L929 cell lines. Also, in MRC-5 fibroblast cells, alkaline phosphatase levels doubled when in contact with HAP/PVA/CS/Gent, indicating good osteogenic properties. The overall construct on the substrate in such a form would be well-advanced arrangement for future medical device improvement of skeletal implants.", publisher = "Belgrade : Serbian Society for Microbiology", journal = "XIII SERBIAN MICROBIOLOGY CONGRESS with international participation, MIKROMED REGIO 5, UMS Series 24: „FROM BIOTECHNOLOGY TO HUMAN AND PLANETARY HEALTH"", title = "Electrochemically synthesized biocomposite poly(vinyl alcohol)/chitosan-based coatings for medical applications", pages = "182-182" }
Janković, A., Đošić, M., Stevanović, M., Kojić, V., Vukašinović-Sekulić, M.,& Mišković-Stanković, V.. (2024). Electrochemically synthesized biocomposite poly(vinyl alcohol)/chitosan-based coatings for medical applications. in XIII SERBIAN MICROBIOLOGY CONGRESS with international participation, MIKROMED REGIO 5, UMS Series 24: „FROM BIOTECHNOLOGY TO HUMAN AND PLANETARY HEALTH" Belgrade : Serbian Society for Microbiology., 182-182.
Janković A, Đošić M, Stevanović M, Kojić V, Vukašinović-Sekulić M, Mišković-Stanković V. Electrochemically synthesized biocomposite poly(vinyl alcohol)/chitosan-based coatings for medical applications. in XIII SERBIAN MICROBIOLOGY CONGRESS with international participation, MIKROMED REGIO 5, UMS Series 24: „FROM BIOTECHNOLOGY TO HUMAN AND PLANETARY HEALTH". 2024;:182-182..
Janković, Ana, Đošić, Marija, Stevanović, Milena, Kojić, Vesna, Vukašinović-Sekulić, Maja, Mišković-Stanković, Vesna, "Electrochemically synthesized biocomposite poly(vinyl alcohol)/chitosan-based coatings for medical applications" in XIII SERBIAN MICROBIOLOGY CONGRESS with international participation, MIKROMED REGIO 5, UMS Series 24: „FROM BIOTECHNOLOGY TO HUMAN AND PLANETARY HEALTH" (2024):182-182.